| Project/Area Number |
21592321
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Niigata University |
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Principal Investigator |
IDA Hiroko 新潟大学, 医歯学系, 准教授 (60293213)
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| Co-Investigator(Kenkyū-buntansha) |
OHSHIMA Hayato 新潟大学, 医歯学系, 教授 (70251824)
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| Co-Investigator(Renkei-kenkyūsha) |
SAKU Takashi 新潟大学, 医歯学系, 教授研究者 (40145264)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 発生・分化 / シグナル伝達 / 歯胚エナメル器 / パールカン |
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| Research Abstract |
Perlecan, a heparan sulfate proteoglycan, plays an important role in cellular growth, differentiation, adhesion and motility by its interaction with growth factors and cytokines. During odontogenesis, perlecan started to be localized in the central area of the epithelial tooth bud, and with the maturation of the enamel organ, it accumulated in the intercellular spaces of the stellate reticulum.
To understand the role of perlecan in enamel organ morphogenesis, we analyzed a keratin 5-perlecan transgenic mice that over-express perlecan in epithelial cells, and examined their tooth germs. The overexpression of perlecan in the enamel organ resulted in irregular morphology of teeth, suggesting that the expression of perlecan regulates growth factor signaling in a stage-dependent manner during each step of the interaction between ameloblast-lineage cells and mesenchymal cells. The time schedule of the intraepithelial expression of perlecan seems to be controlled critically in the process of odontogenesis.
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