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The role of PKR, Osterix, and Calcineurin in osteogenesis

Research Project

Project/Area Number 21592330
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionThe University of Tokushima

Principal Investigator

HANEJI Tatsuji  徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (50156379)

Co-Investigator(Kenkyū-buntansha) TERAMACHI Jyumpei  徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (20515986)
NAKASHIMA Yoshiki  徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (30593082)
Co-Investigator(Renkei-kenkyūsha) MORIMOTO Hiroyuki  産業医科大学, 医学部, 准教授 (30335806)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords蛋白質脱リン酸化 / 蛋白質リン酸化 / 破骨細胞 / 骨吸収 / 軟骨細胞 / PKR / RANKL / RAW264.7細胞 / タンパク質脱リン酸化 / タンパク質リン酸化 / 骨形成 / オステリックス / カルシニューリン / オカダ酸 / カリクリンA
Research Abstract

A dominant-negative mutant PKR cDNA, in which the amino acid lysine at 296 was replaced with arginine, was transfected into RAW264. 7 cells. RANKL stimulated the TRAP-positive multinuclear cell formation in RAW264. 7 cells. However, TRAP-positive multinuclear cells were not formed in the PKR-K/ R mutant cells even when the cells were stimulated with higher doses of RANKL. A specific inhibitor of PKR, 2-aminopurine, also suppressed the RANKL-induced osteoclast differentiation in RAW264. 7 cells. Immunohistochemical study shows that PKR localized in osteoclasts of metatarsal bone of newborn mouse.
ATDC-5 cells differentiated into chondrocytes by the insulin-treatment and the cells produced Alcian blue-positive cartilage matrix. When the cells were treated with 2-aminopurine, formation of cartilage matrix decreased in a dose dependent manner. Protein expression of STAT1, Osterix, and Sox-9 showed different manners during chondrogenesis. We also showed that PKR localized in a marginal region of mandibular condyle cartilage in mouse embryo.
Our findings suggest that PKR plays important roles in the differentiation of osteoclasts and chondrocytes by modulating STAT1, OSX, and Sox-9 expressions.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (52 results)

All 2012 2011 2010 2009

All Journal Article (19 results) (of which Peer Reviewed: 18 results) Presentation (32 results) Book (1 results)

  • [Journal Article] Interaction of protein phosphatase 1δwith nucleophosmin in human osteoblastic cells2012

    • Author(s)
      Haneji T, Teramachi J, Hirashima K, Kimura K, Morimoto H
    • Journal Title

      Acta Histochemica et Cytochemica

      Volume: 45 Pages: 1-7

    • NAID

      120006877868

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 蛋白質リン酸化と脱リン酸化による骨形成と骨吸収2012

    • Author(s)
      羽地達次
    • Journal Title

      四国歯学会雑誌

      Volume: 24 Pages: 45-50

    • NAID

      120005832461

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intermedilysin induces EGR-1 expression through calcineurin/NFAT pathway in human cholansiocellular carcinoma cells2012

    • Author(s)
      Susilowati H, et al
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 404 Pages: 57-61

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reduction of protein phosphatase 2A C・enhances bone formation and osteoblast differentiation through the expression of bone-specific transcription factor Osterix2012

    • Author(s)
      Okamura, et al
    • Journal Title

      Bone

      Volume: 49 Issue: 3 Pages: 368-375

    • DOI

      10.1016/j.bone.2011.06.004

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interaction of Protein Phosphatase 1δ with Nucleophosmin in Human Osteoblastic Cells2012

    • Author(s)
      Haneji, et al
    • Journal Title

      ACTA HISTOCHEMICA ET CYTOCHEMICA

      Volume: 45 Issue: 1 Pages: 1-7

    • DOI

      10.1267/ahc.11041

    • NAID

      130001258485

    • ISSN
      0044-5991, 1347-5800
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intermedilysin induces EGR-1 expression through calcineurin/ NFAT pathway in human cholangiocellular carcinoma cells2011

    • Author(s)
      Susilowati H, Okamura H, Hirota K, Shono M, Yoshida K, Murakami K, Tabata A, Nagamune H, Haneji T, Miyake Y
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 404 Pages: 57-61

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reduction of protein phosphatase 2A Cαenhances bone formation and osteoblast differentiation through the expression of bone-specific transcription factor Osterix Bone2011

    • Author(s)
      Okamura H, Yoshida K, Ochiai K, Haneji T
    • Volume
      49
    • Pages
      368-375
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Intermedilysin induces EGR-1 expression through calcineurin/NFAT pathway in human cholangiocellular carcinoma cells2011

    • Author(s)
      Susilowati Heni
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 404 Pages: 57-61

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High glucose levels increase osteopontin production and pathologic calcification in rat dental pulp tissues2010

    • Author(s)
      Inagaki Y, Yoshida K, Ohba H, Seto H, Kido J, Haneji T, Nagata T
    • Journal Title

      Journal of Endodontics

      Volume: 36 Pages: 1014-1020

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Double stranded RNA-dependent protein kinase is involved in osteoclast differentiation of RAW264. 7 cells in vitro2010

    • Author(s)
      Teramachi J, Morimoto H, Baba R, Doi Y, Hirashima K, Haneji T
    • Journal Title

      Experimental Cell Research

      Volume: 316 Pages: 3254-3262

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional expression of double-stranded RNA-dependent protein kinase in rat intestinal epithelial cells2010

    • Author(s)
      Sato N, Morimoto H, Baba R, Nakamata J, Doi Y, Yamaguchi K
    • Journal Title

      Journal of Cellular Biochemistry

      Volume: 110 Issue: 1 Pages: 104-111

    • DOI

      10.1002/jcb.22513

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Double stranded RNA-dependent protein kinase is involved in osteoclast differentiation of RAW264.7 cells in vitro2010

    • Author(s)
      Teramachi Junpei
    • Journal Title

      Experimental Cell Research

      Volume: 316 Pages: 3254-3262

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Calcineurin regulates phosphorylation status of transcription factor osterix2009

    • Author(s)
      Okamura H, Amorim BR, Wang J, Yoshida K, Haneji T
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 379 Pages: 440-444

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] PKR-mediated degradation of STAT1 regulates osteoblast differentiation2009

    • Author(s)
      Yoshida K, Okamura H, Amorim BR, Hinode D, Yoshida H, Haneji T
    • Journal Title

      Experimental Cell Research

      Volume: 315 Pages: 2105-2114

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Negative regulation of TIMP1 is mediated by transcription factor TWIST12009

    • Author(s)
      Okamura H, Yoshida K, Haneji T
    • Journal Title

      International Journal of Oncology

      Volume: 35 Issue: 01 Pages: 181-186

    • DOI

      10.3892/ijo_00000327

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Calcineurin regulates phosphorylation status of transcription factor osterix2009

    • Author(s)
      Okamura Hirohiko
    • Journal Title

      Biochem Biophys Res Commun 379

      Pages: 440-444

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PKR-mediated degradation of STAT1 regulates osteoblast differentiation2009

    • Author(s)
      Yoshida Kaya
    • Journal Title

      Experimental Cell Research 315

      Pages: 2105-2114

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Negative regulation of TIMP1 is mediated by transcription factor TWIST12009

    • Author(s)
      Okamura Hirohiko
    • Journal Title

      International Journal of Oncology 35

      Pages: 181-186

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2009

    • Author(s)
      羽地達次
    • Journal Title

      培養細胞の免疫蛍光抗体法,組織細胞化学2009(日本組織細胞化学会 中西印刷,京都市)

      Pages: 19-24

    • Related Report
      2009 Annual Research Report
  • [Presentation] 破骨細胞の形成とPKR活性2012

    • Author(s)
      羽地達次, 他
    • Organizer
      第117回日本解剖学会総会
    • Place of Presentation
      信州大学(山梨県)
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] 破骨細胞の形成とPKR活性2012

    • Author(s)
      羽地達次, 寺町順平, 中島義基, 森本景之
    • Organizer
      第117回日本解剖学会総会
    • Place of Presentation
      甲府市(山梨大学)
    • Year and Date
      2012-03-27
    • Related Report
      2011 Final Research Report
  • [Presentation] PKR is required for osteoclast differentiation2011

    • Author(s)
      Haneji T, et al
    • Organizer
      The 10th China-Japan Joint Seminar on Histochemistry and Cytochemistry
    • Place of Presentation
      首都医科大学(北京,中国)(招待講演)
    • Year and Date
      2011-10-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] PKR活性阻害と破骨細胞形成2011

    • Author(s)
      羽地達次, 寺町順平, 中島義基, 森本景之
    • Organizer
      第53回歯科基礎医学会総会
    • Place of Presentation
      岐阜市(長良川国際会議場)
    • Year and Date
      2011-10-02
    • Related Report
      2011 Final Research Report
  • [Presentation] PKR活性阻害と破骨細胞形成2011

    • Author(s)
      羽地達次, 他
    • Organizer
      第53回歯科基礎医学会総会
    • Place of Presentation
      長良川国際会議場(岐阜県)
    • Year and Date
      2011-10-01
    • Related Report
      2011 Annual Research Report
  • [Presentation] 小腸上皮細胞におけるPKRを介したアポトーシスの誘導2011

    • Author(s)
      森本景之, 他
    • Organizer
      第53回歯科基礎医学会総会
    • Place of Presentation
      長良川国際会議場(岐阜県)
    • Year and Date
      2011-10-01
    • Related Report
      2011 Annual Research Report
  • [Presentation] 骨芽細胞分化におけるPP2A Caの新たな役割2011

    • Author(s)
      岡村裕彦, 他
    • Organizer
      第53回歯科基礎医学会総会
    • Place of Presentation
      長良川国際会議場(岐阜県)
    • Year and Date
      2011-10-01
    • Related Report
      2011 Annual Research Report
  • [Presentation] RANKLに誘導される破骨細胞の分化におけるJmjd3-IF4シグナルの役割2011

    • Author(s)
      沖島義基, 他
    • Organizer
      第53回歯科基礎医学会総会
    • Place of Presentation
      長良川国際会議場(岐阜県)
    • Year and Date
      2011-10-01
    • Related Report
      2011 Annual Research Report
  • [Presentation] 破骨細胞形成におけるPKR2011

    • Author(s)
      羽地達次, 寺町順平
    • Organizer
      第52回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      金沢市(金沢大学)
    • Year and Date
      2011-09-24
    • Related Report
      2011 Final Research Report
  • [Presentation] 破骨細胞形成におけるPKR2011

    • Author(s)
      羽地達次, 他
    • Organizer
      第52回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      金沢大学(石川県)
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] オカダ酸誘導アポトーシスとB23の分解2011

    • Author(s)
      平島寛司, 他
    • Organizer
      第30回分子病理研究会
    • Place of Presentation
      鷲羽山下電ホテル(岡山県)
    • Year and Date
      2011-07-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] 蛋白質脱リン酸化反応とアポトーシス2011

    • Author(s)
      羽地達次, 他
    • Organizer
      日本顕微鏡学会第67回学術講演会
    • Place of Presentation
      福岡国際会議場(福岡県)(招待講演)
    • Year and Date
      2011-05-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Calcineurin regulates phosphorylation status of Osterix at Serine 73 site2011

    • Author(s)
      Okamura Hirohiko
    • Organizer
      第116回日本解剖学会総会
    • Place of Presentation
      横浜市
    • Year and Date
      2011-03-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] Calcineurin regulates phosphorylation status of Osterix at Serine73 site2011

    • Author(s)
      Okamura H, Haneji T
    • Organizer
      第116回日本解剖学会総会
    • Place of Presentation
      横浜市(パシフィコ横浜)
    • Year and Date
      2011-03-29
    • Related Report
      2011 Final Research Report
  • [Presentation] Localization of protein phosphatase 1δ and induction of apoptosis with a protein phosphatase inhibitor okadaic acid2011

    • Author(s)
      Hirashima Kanji
    • Organizer
      第116回日本解剖学会総会
    • Place of Presentation
      横浜市
    • Related Report
      2010 Annual Research Report
  • [Presentation] RANKL刺激による破骨細胞分化におけるPKRの役割2010

    • Author(s)
      森本景之
    • Organizer
      日本顕微鏡学会第52回九州支部学術講演会
    • Place of Presentation
      福岡市
    • Year and Date
      2010-12-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] オカダ酸誘導アポトーシスとB23の分解2010

    • Author(s)
      平島寛司
    • Organizer
      日本解剖学会第65回中国・四国支部学術集会
    • Place of Presentation
      呉市
    • Year and Date
      2010-10-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] PKR活性阻害が軟骨細胞分化に及ぼす影響2010

    • Author(s)
      森本景之
    • Organizer
      第52回歯科基礎医学会総会
    • Place of Presentation
      東京都
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] PKR活性阻害が軟骨細胞分化に及ぼす影響2010

    • Author(s)
      森本景之, 寺町順平, 羽地達次
    • Organizer
      第52回歯科基礎医学会総会
    • Place of Presentation
      船橋市(タワーホール船堀)
    • Year and Date
      2010-09-21
    • Related Report
      2011 Final Research Report
  • [Presentation] PKRは破骨細胞形成を誘導する2010

    • Author(s)
      寺町順平
    • Organizer
      第52回歯科基礎医学会総会
    • Place of Presentation
      東京都
    • Year and Date
      2010-09-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] 蛋白質脱リン酸化酵素PP1δとB23の細胞内局在およびアポトーシス細胞におけるB23の分解2010

    • Author(s)
      羽地達次
    • Organizer
      第51回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      東京都
    • Year and Date
      2010-09-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] 蛋白質脱リン酸化酵素PP1δとB23の細胞内局在およびアポトーシス細胞におけるB23の分解2010

    • Author(s)
      羽地達次, 寺町順平, 木村幸司, 平島寛司, 森本景之, 土肥良秋
    • Organizer
      第51回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      東京都(秋葉原コンペティションホール)
    • Year and Date
      2010-09-04
    • Related Report
      2011 Final Research Report
  • [Presentation] 消化管上皮における二本鎖RNA依存プロテインキナーゼ(PKR)の局在2010

    • Author(s)
      森本景之
    • Organizer
      第29回分子病理研究会
    • Place of Presentation
      つくば市
    • Year and Date
      2010-08-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] 基礎系教育講演「蛋白質リン酸化と脱リン酸化による骨形成と骨吸収」2010

    • Author(s)
      羽地達次
    • Organizer
      第29回四国歯学会総会
    • Place of Presentation
      徳島市
    • Year and Date
      2010-07-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] 高血糖は歯髄細胞におけるオステオポンチン産生と病的石灰化を促進する2010

    • Author(s)
      稲垣裕司
    • Organizer
      第1回骨バイオサイエンス研究会
    • Place of Presentation
      岡山市
    • Year and Date
      2010-06-13
    • Related Report
      2010 Annual Research Report
  • [Presentation] Bleomycin-induced apoptosis and Histone H1.22009

    • Author(s)
      Haneji Tatsuji
    • Organizer
      The 9^<th> China-Japna Joint Seminar on Histochemistry and Cytochemistry
    • Place of Presentation
      広西大学医学部(南寧市・中国)
    • Year and Date
      2009-11-06
    • Related Report
      2009 Annual Research Report
  • [Presentation] PKRは破骨細胞形成に重要な分子である2009

    • Author(s)
      寺町順平
    • Organizer
      日本解剖学会 第64回中国・四国支部学術集会
    • Place of Presentation
      高知大学医学部(高知県)
    • Year and Date
      2009-10-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] PKR変異型骨芽細胞株における蛋白質脱リン酸化酵素阻害剤によるアポトーシス誘導とIκBの発現2009

    • Author(s)
      羽地達次, 寺町順平, 岡村裕彦, 吉田賀弥, 森本景之
    • Organizer
      第51回歯科基礎医学会総会
    • Place of Presentation
      新潟市(白鷺メッセ)
    • Year and Date
      2009-09-11
    • Related Report
      2011 Final Research Report
  • [Presentation] PKR変異型骨芽細胞株における蛋白質脱リン酸化酵素阻害剤によるアポトーシス誘導と IκBの発現2009

    • Author(s)
      羽地達次
    • Organizer
      第51回歯科基礎医学会総会
    • Place of Presentation
      朱鷺メッセ(新潟県)
    • Year and Date
      2009-09-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] カリクリンAによるMG63細胞のアポトーシス誘導, I-κBの分解, NF-κBリン酸化2009

    • Author(s)
      羽地達次, 田中宏明, 岡村裕彦, 吉田賀弥, 森本景之
    • Organizer
      日本Cell Death学会第18回学術集会
    • Place of Presentation
      長崎市(長崎大学)
    • Year and Date
      2009-08-02
    • Related Report
      2011 Final Research Report
  • [Presentation] カリクリンAによるMG63細胞のアポトーシス誘導, I-κBの分解, NF-κBのリン酸化2009

    • Author(s)
      羽地達次
    • Organizer
      日本 Cell Death 学会 第18回学術集会
    • Place of Presentation
      長崎大学医学部・良順会館(長崎県)
    • Year and Date
      2009-08-02
    • Related Report
      2009 Annual Research Report
  • [Presentation] 蛋白質脱リン酸化酵素阻害剤によるアポトーシス誘導における蛋白質相互作用2009

    • Author(s)
      森本景之
    • Organizer
      日本 Cell Death 学会 第18回学術集会
    • Place of Presentation
      長崎大学医学部・良順会館(長崎県)
    • Year and Date
      2009-08-02
    • Related Report
      2009 Annual Research Report
  • [Book] 培養細胞の免疫蛍光抗体法,組織細胞化学20092009

    • Author(s)
      羽地達次, 寺町順平
    • Publisher
      日本組織細胞化学会
    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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