Functional analysis for molecular networks of thymosin beta 4 in developing tooth germ.

• Project/Area Number: 21592331
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Kyushu University
• Principal Investigator: KOBAYASHI Ieyoshi 九州大学, 歯学研究院, 准教授 (40243951)
• Co-Investigator(Kenkyū-buntansha): SAKAI Hidetaka 九州大学, 歯学研究院, 教授 (80136499) ; NAGATA Kengo 九州大学, 歯学研究院, 助教 (90189134) ; KIYOSHIMA Tamotsu 九州大学, 歯学研究院, 講師 (20264054)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 口腔病理学 / 歯の発生 / 基底膜 / 歯胚 / 細胞・組織 / 歯学 / ノックダウンアッセイ / アンチセンス法 / 器官培養

Research Abstract

We examined the functional implications of Tβ4 in the developmental course of the mouse lower first molar. An inhibition assay using Tβ4 antisense sulfur-substituted oligodeoxynucleotide(AS S-ODN) in cultured embryonic day 11. 0(E11. 0) mandibles showed a significant growth inhibition of the tooth germ. The Tβ4 knockdown led to decreased expression levels of type II/III Runx2 and nucleolin(Ncl) in the cultured E11. 0 mandibles. Significant down-regulation of them was also observed in cultured mouse odontogenic epithelial cell. Tβ4 appears to play roles in tooth germ development via the regulation of the type II/III Runx2 and Ncl expressions. These results provided new evidence that Tβ4 is involved in the functions of odontogenic epithelial cells in the early stage of tooth germ development by regulating the expression of odontogenesis-related genes.

Research Products

• [Journal Article] 左側上顎に発生した石灰化嚢胞性歯原性腫瘍の1例 (2011)
  • Author(s): 藤原弘明、小林家吉、清島保、永田健吾、和田裕子、大隈由紀子、塩塚真帆、木原槇子、坂井英隆
  • Journal Title: 診断病理 Volume: 28巻1号 Pages: 117-121
  • NAID: 10029411792
  • Peer Reviewed
• [Journal Article] In situ expression of the mitochondrial ATPase6 gene in the developing tooth germ of the mouse lower first molar (2011)
  • Author(s): Honda JY, Kobayashi I, Kiyoshima T, Nagata K, Wada H, Ookuma Y, Fujiwara H, Shiotsuka M, Takahashi I, Sakai H
  • Journal Title: Journal Molecular Histology Volume: Vol.42 No.1 Pages: 83-90
  • Peer Reviewed
• [Journal Article] 左側上顎に発生した石灰化嚢胞性歯原性腫瘍の1例 (2011)
  • Author(s): 藤原弘明, 他8名
  • Journal Title: 診断病理 Volume: 28 Pages: 117-121
  • Peer Reviewed
• [Journal Article] In situ expression of the mitochondrial ATPase6 gene in the developing tooth germ of the mouse lower first molar (2011)
  • Author(s): Jun-ya Honda, et al.
  • Journal Title: Journal of Molecular Histology Volume: 42 Pages: 83-89
  • Peer Reviewed
• [Journal Article] Protogenin, a new member of the immunoglobulin superfamily, is implicated in the development of the mouse lower first molar (2010)
  • Author(s): Takahashi KF, Kiyoshima T, Kobayashi I, Xie M, Yamaza H, Fujiwara H, Ookuma Y, Nagata K, Wada H, Sakai T, Terada Y, Sakai H
  • Journal Title: BMC Developmental Biology Volume: Vol.10 No. Pages: 115-115
  • Peer Reviewed
• [Journal Article] In situ expression of 15 kDa interferon alpha responsive gene in the developing tooth germ of the mouse lower first molar (2010)
  • Author(s): Akhter M, Kobayashi I, Kiyoshima T, Nagata K, Wada H, Ookuma Y, Fujiwara H, Honda JY, Sakai H
  • Journal Title: Journal of Molecular Histology Volume: Vol.41 No.4-5 Pages: 185-191
  • NAID: 120005227146
  • Peer Reviewed
• [Journal Article] In situ expression of ribosomal protein L21 in developing tooth germ of the mouse lower first molar (2010)
  • Author(s): Ming Xie, Ieyoshi Kobayashi, Tamotsu Kiyoshima, Kengo Nagata, Yukiko Ookuma, Hiroaki Fujiwara, Hidetaka Sakai
  • Journal Title: Journal of Molecular Histology Volume: Vol.40 No.5-6 Pages: 361-367
  • Peer Reviewed
• [Journal Article] In situ expression of 15 kDa interferon alpha responsive gene in the developing tooth germ of the mouse lower first molar (2010)
  • Author(s): Merina Akhter, et al.
  • Journal Title: Journal of Molecular Histology Volume: 41 Pages: 185-191
  • Peer Reviewed
• [Journal Article] In situ expression of ribosomal protein L21 in developing tooth germ of the mouse lower first molar (2009)
  • Author(s): Ming Xie, et al.
  • Journal Title: Journal of Molecular Histology 31 Pages: 361-367
  • Peer Reviewed
• [Presentation] 癌治療用アポトーシス誘導ベクターの作成と導入の試み (2011)
  • Author(s): 古庄克弘, 他7名
  • Organizer: 第53回歯科基礎医学会学術大会ならびに総会
  • Place of Presentation: 岐阜
  • Year and Date: 2011-10-02
• [Presentation] Soft tissue tumor of the lower lip (2011)
  • Author(s): 和田裕子, 他5名
  • Organizer: 第22回日本臨床口腔病理学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2011-08-24
• [Presentation] Functional implication of thymosin beta-4 in the morphogenesis of the tooth germ via the regulation of odontogenic gene expressions (2011)
  • Author(s): Y.Ookuma, 他7名
  • Organizer: The 6th International Joint Symposium on "Dental and Craniofacial Morphogenesis and Tissue Regeneration" and "Oral Health Science"
  • Place of Presentation: 福岡
  • Year and Date: 2011-03-04
• [Presentation] Expression pattern and possible function of thymosin beta-10 in developing tooth germ compared with thymosin beta-4 (2011)
  • Author(s): M.Shiotsuka, 他7名
  • Organizer: The 6th International Joint Symposium on "Dental and Craniofacial Morphogenesis and Tissue Regeneration" and "Oral Health Science"
  • Place of Presentation: 福岡
  • Year and Date: 2011-03-04
• [Presentation] Thymosin beta 4のノックダウンによる歯原性細胞の変化について (2011)
  • Author(s): 小林家吉、清島保、永田健吾、和田裕子、藤原弘明、塩塚真帆、坂井英隆
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜
• [Presentation] 歯胚形成過程におけるThymosin b-10の役割～Thymosin b-4との比較検討～ (2011)
  • Author(s): 和田裕子、塩塚真帆、清島保、小林家吉、永田健吾、藤原弘明、坂井英隆
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜
• [Presentation] Expression pattern and possible function of thymosin beta-10 in developing tooth germ compared with thymosin beta-4 (2011)
  • Author(s): Maho Shiotsuka, Hiroko Wada, Tamotsu Kiyoshima, Ieyoshi Kobayashi, Kengo Nagata, Hiroaki Fujiwara, Ichiro Takahashi and Hidetaka Sakai
  • Organizer: The 6th International Joint Symposium on"Dental and Craniofacial Morphogenesis and Tissue Regeneration"and"Oral Health Science"
  • Place of Presentation: 福岡
• [Presentation] Functional implication of thymosin beta-4 in the morphogenesis of the tooth germ via the regulation of odontogenic gene expressions (2011)
  • Author(s): Yukiko Ookuma, Ieyoshi Kobayashi, Tamotsu Kiyoshima, Kengo Nagata, Hiroko Wada, Kazuaki Nonaka and Hidetaka Sakai
  • Organizer: The 6th International Joint Symposium on"Dental and Craniofacial Morphogenesis and Tissue Regeneration"and"Oral Health Science
  • Place of Presentation: 福岡
• [Presentation] マウス歯胚形成過程におけるThymosin・10とThymosin・4との発現様式の比較検討 (2010)
  • Author(s): 和田裕子, 他8名
  • Organizer: 第52回歯科基礎医学会学術大会ならびに総会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-22
• [Presentation] ヒストン脱アセチル化酵素阻害剤suberoylanilide hydroxamic acidによるヒト口腔扁平上皮癌細胞のアポトーシス誘導 (2010)
  • Author(s): 永田健吾, 他8名
  • Organizer: 第52回歯科基礎医学会学術大会ならびに総会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-22
• [Presentation] Functional implication of Thymosin beta 4 in the tooth development (2010)
  • Author(s): Y.Ookuma, 他7名
  • Organizer: The 88th General Session & Exhibition of the IADR
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-17
• [Presentation] IL-22によるヒト口腔扁平上皮癌の細胞形質に対する影響 (2010)
  • Author(s): 清島保, 他4名
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京
  • Year and Date: 2010-04-29
• [Presentation] ヒト口腔扁平上皮癌細胞のヒストン脱アセチル化酵素阻害剤によるアポトーシス誘導 (2010)
  • Author(s): 永田健吾, 他4名
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京
  • Year and Date: 2010-04-28
• [Presentation] マウス歯胚形成過程におけるThymosin beta 10とThymosin beta 4との発現様式の比較検討 (2010)
  • Author(s): 和田裕子、清島保、小林家吉、塩塚真帆、永田健吾、大隈由紀子、藤原弘明、高橋一郎、坂井英隆
  • Organizer: 第52回歯科基礎医学会学術大会
  • Place of Presentation: 東京
• [Presentation] Functional implication of Thymosin beta 4 in the tooth development (2010)
  • Author(s): Yukiko Ookuma, Ieyoshi Kobayashi, Tamotsu Kiyoshima, Kengo Nagata, Hiroaki Fujiwara, Haruyoshi Yamaza, Kazuaki Nonaka, Hidetaka Sakai
  • Organizer: 88th IADR General Session
  • Place of Presentation: バルセロナ
• [Presentation] 歯胚の形態形成における Thymosin beta 4の機能的役割について (2009)
  • Author(s): 大隈由紀子, 他2名
  • Organizer: 第86回九大病理研究会
  • Place of Presentation: 福岡
  • Year and Date: 2009-12-12
• [Presentation] Tumor of the Mandible (2009)
  • Author(s): Yoshinao Oda, 他5名
  • Organizer: Closed Meeting of the 36^<th> International Skeletal Society
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2009-08-31
• [Presentation] IgG4関連硬化性疾患における生検小唾液腺の病理組織学的所見 (2009)
  • Author(s): 清島保, 他3名
  • Organizer: 第20回日本臨床口腔病理学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2009-07-30
• [Presentation] ヒト篇平上皮癌におけるIL-22シグナル経路 (2009)
  • Author(s): 清島保, 他2名
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 京都
  • Year and Date: 2009-05-03
• [Presentation] 歯胚形成過程におけるRpl21およびIfrg15の発現様式について (2009)
  • Author(s): 小林家吉、清島保、永田健吾、大隈由紀子、藤原弘明、坂井英隆
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 新潟