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Regulation of inflammatory bone destruction by galectin 3 : Identification of the novel receptor and molecular histological research

Research Project

Project/Area Number 21592332
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionKyushu University

Principal Investigator

LI Yinji  九州大学, 歯学研究院, 助教 (90457424)

Co-Investigator(Kenkyū-buntansha) 久木田 敏夫 (久木田 明子)  佐賀大学, 医学部, 准教授 (30153266)
久木田 敏夫  九州大学, 歯学研究院, 教授 (70150464)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords口腔解剖学(含組織学・発生学) / ガレクチン3 / 糖鎖結合ドメイン / N末端領域 / 破骨細胞分化 / タンデム型ガレクチン / 膜表面受容体 / 炎症性骨破壊 / 組み替え蛋白質 / 新規レセプター
Research Abstract

Galectin 3 is the factor which regulates inflammatory bone destruction and suppresses osteoclastogenesis. As the inhibitory activity of galectin 3 was not attributed to the carbohydrate recognition domain(CRD), N-terminal region was considered to have regulatory roles in the regulation of osteoclastogenesis. N-terminal region of galectin-3 was recloned and recombinant protein was obtained, however, we have failed in obtaining active recombinant polypeptides bearing inhibitory activity on osteoclastogenesis. We then examined other types of galectin. Galectin 9 is the tandem type of galectin with two CRDs connected by a linker polypeptide. Galectin 1 is the prototype galectin composed of only two CRD. Although galectin 1 did not affect osteoclastogenesis, galectin 9 markedly inhibited osteoclastogenesis in a dose-dependent manner. The inhibition of osteoclastogenesis by galectin 9 was partly canceled by the addition of lactose. It was strongly suggested the presence of CRD-dependent mechanism as well as CRD-independent mechanism. We further examined if the galectin 9 receptor is expressed in osteoclast precursors and found that some receptor molecules regulating apoptosis are expressed in osteoclast precursors. This research would provide important tool and strategy for controlling inflammatory bone destruction.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (21 results)

All 2011 2010 2009

All Journal Article (7 results) (of which Peer Reviewed: 3 results) Presentation (14 results)

  • [Journal Article] The Transcription Factor FBI-1/OCZF/LRF Is Expressed in Osteoclasts and Regulates RANKL-Induced Osteoclast Formation In Vitro and In Vivo2011

    • Author(s)
      Kukita A., Kukita T., , Nagata K., Teramachi J., Li Y-J., Yoshida H., Miyamoto H., Gay S., Pessler F., Shobuike T.
    • Journal Title

      Arth Rheumatism

      Volume: 63(9) Pages: 2744-2754

    • Related Report
      2011 Final Research Report
  • [Journal Article] Adenosine abolishes MTX-induced suppression of osteoclastogenesis and inflammatory bone destruction in adjuvant-induced arthritis2011

    • Author(s)
      Teramachi J., Kukita A., Li Y-J., Ushijima Y., Ohkuma Y., , Wada N., Watanabe T., Nakamura S., Kukita T.
    • Journal Title

      Lab. Invest

      Volume: 91 Pages: 719-731

    • Related Report
      2011 Final Research Report
  • [Journal Article] The transcription factor FBI-1/OCZF/LRF is expressed in osteoclasts and regulates RANKL-induced osteoclast formation in vitro and in vivo2011

    • Author(s)
      Kukita A., Kukita T., Nagata K., Teramachi J., Li Y-J., Yoshida H., Miyamoto H., Gay S., Pessler F., Shoubuike T.
    • Journal Title

      Arthritis and Rheumatism

      Volume: 63(9) Pages: 2744-2754

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Adenosine abolishes MTX-induced suppression of osteoclastogenesisi and unflammatory bone destruction in adjuvant-induced arthritis2011

    • Author(s)
      Teramachi J., Kukita A., Li Y-J., Ushijima Y., Ohkuma H., Wada N., Watanabe T., Nakamura S., Kukita T.
    • Journal Title

      Laboratory Inveswtigation

      Volume: 91 Pages: 719-731

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Adenosine abolishes MTX-induced suppression of osteoclasto-genesis and inflammatory bone destruction in adjuvant-induced arthritis2011

    • Author(s)
      Junpei Teramachi, Akiko Kukita, Yin-Ji Li, Yuki Ushijima, Hiroshi Ohkuma, Naohisa Wada, Toshiyuki Watanabe, Seiji Nakamura, Toshio Kukita
    • Journal Title

      Laboratory Investigation

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phosphatidylserine-containing liposomes inhibit the differentiation of osteoclasts and trabecular bone loss2010

    • Author(s)
      Wu Z., Ma H. M., Kukta T., Nakanishi Y., Nakanishi H. J. Immunol.
    • Volume
      184(6)
    • Pages
      3191-3201
    • Related Report
      2011 Final Research Report
  • [Journal Article] A possible suppressive role of galectin-3 in up-regulated osteoclastogenesis accompanying adjuvant-induced arthritis in rats2009

    • Author(s)
      Li Y-J., Kukita A., Teramachi J., Nagata K., Wu Z., Akamine A., Kukita T.
    • Journal Title

      Lab. Invest

      Volume: 89 Pages: 26-37

    • Related Report
      2011 Final Research Report
  • [Presentation] 間葉系幹細胞による炎症性骨破壊制御2011

    • Author(s)
      高野登志夫、李銀姫、久木田明子、山座孝義、高橋明、鮎川保則、古谷野潔、久木田敏夫
    • Organizer
      第53回歯科基礎医学会
    • Place of Presentation
      岐阜
    • Related Report
      2011 Final Research Report
  • [Presentation] 膜ナノチューブによる前破骨細胞融合制御2011

    • Author(s)
      高橋良、久木田明子、李銀姫、鮎川保則、古谷野潔、久木田敏夫
    • Organizer
      第53回歯科基礎医学会
    • Place of Presentation
      岐阜
    • Related Report
      2011 Final Research Report
  • [Presentation] ヒト過剰歯由来幹細胞の免疫細胞療法効果について2011

    • Author(s)
      牧野友祐、山座孝義、山座治義、馬蘭、益田啓太郎、園田総一朗、城戸瑞穂、野中和明、寺田善博、久木田敏夫
    • Organizer
      第53回歯科基礎医学会
    • Place of Presentation
      岐阜
    • Related Report
      2011 Final Research Report
  • [Presentation] Mesenchyme stem cells markedly suppressed inflammatory bone destruction in rats with adjuvant-induced arthritis2011

    • Author(s)
      Toshio Takano, Yin-ji Li, Akiko Kukita, Takayoshi Yamaza, Yasunori Ayukawa, Kiyoshi Koyano, Toshio Kukita.
    • Organizer
      American Society for Bone and Mineral Research(ASBMR) Annual Meeting
    • Place of Presentation
      (SanDiego, USA)
    • Related Report
      2011 Final Research Report
  • [Presentation] Involement and regulatory role of membrane nanotubes in fusion ofosteoclast precursors:2011

    • Author(s)
      Akira Takahashi, Akiko Kukita, Yin-ji Li, Hisayuki Nomiyama, Yasunori Ayukawa, Kiyoshi Koyano, Toshio Kukita
    • Organizer
      A possible migration and penetration of DC-STAMP protein into osteoclast precursors through intercellular bridges. American Society for Bone and Mineral Research(ASBMR) Annual Meeting
    • Place of Presentation
      (SanDiego, USA)
    • Related Report
      2011 Final Research Report
  • [Presentation] Nordihydroguaiaretic Acidは破骨細胞分化とラットアジュバント関節炎における骨破壊を抑制する2011

    • Author(s)
      李銀姫、久木田明子、屈鵬飛、高野登志夫,實松敬介、二ノ宮祐三、久木田敏夫
    • Organizer
      第29回日本骨代謝学会
    • Place of Presentation
      大阪
    • Related Report
      2011 Final Research Report
  • [Presentation] 間葉系幹細胞による骨破壊制御:アジュバント関節炎ラットを用いた解析2011

    • Author(s)
      高野登志夫、李銀姫、久木田明子、山座孝義、鮎川保典小谷野潔、久木田敏夫
    • Organizer
      第29回日本骨代謝学会
    • Place of Presentation
      大阪
    • Related Report
      2011 Final Research Report
  • [Presentation] アンチセンスWT1 RNAによる破骨細胞分化状態維持機構の可能性について2010

    • Author(s)
      李銀姫、久木田明子、久木田敏夫
    • Organizer
      第52回歯科基礎医学会
    • Place of Presentation
      東京 タワーホール船堀
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] 破骨細胞分化制御に於けるWilms'腫瘍遺伝子産物WT1とそのアンチセンスRNAの関与2010

    • Author(s)
      李銀姫、久木田明子、久木田敏夫
    • Organizer
      第28回日本骨代謝学会
    • Place of Presentation
      東京 京王プラザホテル
    • Year and Date
      2010-07-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] 乳歯凍結保存法がヒト乳歯由来幹細胞の免疫調節能に与える影響について2010

    • Author(s)
      山座孝義、牧野友祐、山座治義、馬蘭、園田総一朗、益田啓太郎、野中和明、寺田善博、久木田敏夫
    • Organizer
      第53回歯科基礎医学会
    • Place of Presentation
      岐阜
    • Related Report
      2011 Final Research Report
  • [Presentation] Nordihydroguaiaretic acid による破骨細胞分化と機能の制御2009

    • Author(s)
      李銀姫, 久木田明子, 久木田敏夫
    • Organizer
      第51回 歯科基礎医学会
    • Place of Presentation
      新潟コンベンションセンター
    • Year and Date
      2009-09-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] Nordihydroguaiaretic acid は破骨細胞の形成と骨吸収を抑制する2009

    • Author(s)
      李銀姫, 久木田明子, 久木田敏夫
    • Organizer
      第27回 日本骨代謝学会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2009-07-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] Nordihydroguaiaretic acidは破骨細胞の形成と骨吸収を抑制する2009

    • Author(s)
      李銀姫、久木田明子、久木田敏夫
    • Organizer
      第27回日本骨代謝学会
    • Place of Presentation
      大阪
    • Related Report
      2011 Final Research Report
  • [Presentation] Nordihydroguaiaretic acidによる破骨細胞分化と機能の制御2009

    • Author(s)
      李銀姫、久木田明子、久木田敏夫
    • Organizer
      第51回歯科基礎医学会
    • Place of Presentation
      新潟
    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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