| Project/Area Number |
21592338
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
SETA Yuji 九州歯科大学, 歯学部, 准教授 (90291616)
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| Co-Investigator(Kenkyū-buntansha) |
TOYOSHIMA Kuniaki 九州歯科大学, 歯学部, 教授 (10112559)
TOYONO Takashi 九州歯科大学, 歯学部, 助教 (10311929)
KATAOKA Shinji 九州歯科大学, 歯学部, 助教 (80364149)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 味蕾 / カドヘリン / 転写制御因子 / Mashl / GAD67 / AADC / マウス / 神経回路形成 / シナプス / ニューレキシン1 / カドヘリン24 / N-カドヘリン / 膜表面分子 / カドヘリンファミリー / III型細胞 |
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| Research Abstract |
Mashl is expressed in subsets of neuronal precursors in both the central nervous system and the peripheral nervous system. However, involvement of Mashl in taste bud cell differentiation remained to be demonstrated. In the present study, to begin to understand the mechanisms that regulate taste bud cell differentiation, we have investigated the role of Mashl in regulating taste bud cell differentiation using Mashl KO mice. In Mashl KO mice, AADC-IR cells are missing both in the Mashl mutant circumvallate papilla epithelium and in the taste buds of soft palate. In Mashl KO/GAD67-GFP mice, GFP-positive(GAD67 expression type III cell) cells are also missing in the taste buds of soft palate. On the other hand, Gustducin, a type II cell marker of taste bud, is expressed in soft palate taste bud in Mashl mutant mice. These results suggest Mashl play an important role for expression of AADC and GAD67 in type III cell in taste buds.
Taste receptor cells are epithelial in sense that they have a limited life span and therefore must be replaced to maintain the structure of the epithelium. Therefore gustatory nerves need to make synapse with appropriate taste receptor cells. However, mechanism of recognition of taste receptor cells which express appropriate taste receptor still unknown. The cadherin superfamily of cell-cell adhesion molecules controls a series of interactions that regulate the dissociation, synapse formation. In this study, in order to test whether the cadherins are required for formation of synapse between gustatory nerve and taste receptor cell, we have investigated expression patterns of cadherin superfamily in the taste buds.
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