| Project/Area Number |
21592343
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Tokyo Dental College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ISHIHARA Kazuyuki 東京歯科大学, 歯学部, 教授 (00212910)
KATO Tetsuo 東京歯科大学, 歯学部, 教授 (00159253)
稲垣 覚 東京歯科大学, 歯学部, 助教 (20385165)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2011: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 口腔細菌学 / 付着 / バイオフィルム / 線毛 / アミロイド / 歯周病 / 歯周病原性菌 / Aggregatibacter actinomycetemcomitans / アミロイド線維 |
|---|
| Research Abstract |
Aggregatibacter actinomycetemocimitans is a pathogen of aggressive periodontitis. This microorganism has strong adherence activity and the ability plays an important role in its virulence. In this project, we intended to identify adherence factor including amyloid-like fiber other than fimbriae.
Amyloid-like fiber was detected by the thioflavin T floorescence assay. The amyloide-like fiber was fractionated with ion-exchange chromatography and gel chromatography. The fiber was stable after heat treatment and solubilization with the acidic buffer. The profile is resembled with the amyloid fiber of Escherichia coli.
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