Investigation of consortia formation by periodontopathic bacteria
Project/Area Number |
21592344
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Morphological basic dentistry
|
Research Institution | Tokyo Dental College |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
KATO Tetsuo 東京歯科大学, 微生物学講座, 教授 (00159253)
KIMIZUKA Ryuta 東京歯科大学, 微生物学講座, 講師 (90287178)
INAGAKI Satoru 東京歯科大学, 微生物学講座, 助教 (20385165)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 口腔細菌学 / バイオフィルム / 歯周病 / 歯周病原性菌 / Synergistic effect / コンソーシア / Fucobacterium nucleatum / 共凝集 / Capnocytophaga ochracea / Prevotella intermedia / Prevotella nigrescence / Autoinducer-2 / 歯周炎 / red complex / プロテアーゼ / カテールアミン / ストレス / 歯周病原細菌 / Polymicrobial infection / Biofilm / Periodontopathic bacteria / Periodontitis / Fusobacterium nucleatum / Porphyromonas gingivalis / Invasion |
Research Abstract |
In this project, our goal was to investigate bacterial interaction in the process of biofilm formation to clarify the virulence of periodontopathic bacteria in dental plaque. In the process of colonization, P. gingivalis adhered to T. denticola by means of Hgp44, which is located on its surface. A synergistic effect was observed in co-culture of F. nucleatum and P. intermedia, C. ochracea, or P. gingivalis. In addition, invasion of epithelial cells by P. gingivalis was enhanced by co-infection with F. nucleatum or P. gingivalis. These results suggest that organization as consortia confers an advantage on periodontopathic bacteria in the formation of dental plaque.
|
Report
(4 results)
Research Products
(64 results)
-
[Journal Article] Transmission of Periodontopathic Bacteria from Natural Teeth to Implants2012
Author(s)
Aoki, M., Takanashi, K., Matsukubo, T., Yajima, Y., Okuda, K., Sato, T., Ishihara, K
-
Journal Title
Clin Implant Dent Relat Res
Volume: 14
Issue: 3
Pages: 406-411
DOI
Related Report
Peer Reviewed
-
[Journal Article] Antibacterial activity of povidone-iodine against an artificial biofilm of Porphyromonas gingivalis and Fusobacterium nucleatum2012
Author(s)
Hosaka, Y., Saito, A., Maeda, R., Fukaya, C., Morikawa, S., Makino, A., Ishihara, K., Nakagawa, T
-
Journal Title
Arch Oral Biol
Volume: 57
Issue: 4
Pages: 364-368
DOI
Related Report
Peer Reviewed
-
[Journal Article] Detection of periodontal bacteria in thrombi of patients with acute myocardial infarction by polymerase chain reaction2012
Author(s)
Ohki, T., Itabashi, Y., Kohno, T., Yoshizawa, A., Nishikubo, S., Watanabe, S., Yamane, G., Ishihara, K
-
Journal Title
Am Heart J
Volume: 163
Issue: 2
Pages: 164-167
DOI
Related Report
Peer Reviewed
-
[Journal Article] Synergy in biofilm formation between Fusobacterium nucleatum and Prevotella species2012
Author(s)
Okuda, T., Kokubu, E., Kawana, T., Saito, A., Okuda, K., Ishihara, K
-
Journal Title
Anaerobe
Volume: 18
Issue: 1
Pages: 110-116
DOI
Related Report
Peer Reviewed
-
[Journal Article] Synergistic effect on biofilm formation between Fusobacterium nucleatum and Capnocytophaga ochracea2012
Author(s)
Okuda, T., Okuda, K., Kokubu, E., Kawana, T., Saito, A., Ishihara, K
-
Journal Title
Anaerobe
Volume: 18
Issue: 1
Pages: 157-161
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Oral bacterial extracts facilitate early osteogenic/dentinogenic differentiation in human dental pulp-derived cells2010
Author(s)
Abe, S., Imaizumi, M., Mikami, Y., Wada, Y., Tsuchiya, S., Irie, S., Suzuki, S., Satomura, K., Ishihara, K., Honda, M. J
-
Journal Title
Oral Surg Oral Med Oral Pathol Oral Radiol Endod
Volume: 109
Issue: 1
Pages: 149-154
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Dentipain, a Streptococcus pyogenes IdeS protease homolog, is a novel virulence factor of Treponema denticola2010
Author(s)
Ishihara, K., Wawrzonek, K., Shaw, L. N., Inagaki, S., Miyamoto, M., Potempa, J
-
Journal Title
Biological chemistry
Volume: 391
Issue: 9
Pages: 1047-1055
DOI
Related Report
Peer Reviewed
-
-
-
-
[Journal Article] Oral environmental factors affecting number of microbes in saliva of complete denture wearers2010
Author(s)
Ryu, M., Ueda, T., Saito, T., Yasui, M., Ishihara, K., Sakurai, K
-
Journal Title
J Oral Rehabil
Volume: 37
Issue: 3
Pages: 194-201
DOI
Related Report
Peer Reviewed
-
[Journal Article] Analysis of matrix metalloproteinase(MMP-8 and MMP-2) activity in gingival crevicular fluid from children with Down's syndrome2010
Author(s)
Yamazaki-Kubota, T., Miyamoto, M., Sano, Y., Kusumoto, M., Yonezu, T., Sugita, K., Okuda, K., Yakushiji, M., Ishihara, K
-
Journal Title
J Periodontal Res
Volume: 45
Issue: 2
Pages: 170-176
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
[Journal Article] Characterization of bacterial flora in persistent apical periodontitis lesions2009
Author(s)
Fujii, R., Saito, Y., Tokura, Y., Nakagawa, K. I., Okuda, K., Ishihara, K
-
Journal Title
Oral Microbiol Immunol
Volume: 24
Issue: 6
Pages: 502-505
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Book] Invasion of Host Cells by Porphyromonas Gingivalis in Polymicrobial Infection, Periodontal Disease : Symptoms, Treatment and Prevention2011
Author(s)
Saito, A., Inagaki, S., Kokubu, E., Kimizuka, R., Ishihara, K, Yamamoto, S. L.
Publisher
Nova Science Publishers, Inc.
Related Report
-
-
-
-
-
-