| Project/Area Number |
21592345
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
AOBA Takaaki 日本歯科大学, 生命歯学部, 教授 (30028807)
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| Co-Investigator(Kenkyū-buntansha) |
TAYA Yuji 日本歯科大学, 生命歯学部, 准教授 (30197587)
SOENO Yuuichi 日本歯科大学, 生命歯学部, 講師 (70350139)
SATO Kaori 日本歯科大学, 生命歯学部, 講師 (90287772)
SHIMAZU Yoshihito 日本歯科大学, 生命歯学部, 講師 (10297947)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 歯学 / 病理学 / マウス胎仔 / 遺伝子発現 / 顎顔面形態形成 / 突起間癒合 / 裂奇形 / 器官発生 / マウスモデル / 顎顔面形成 / 上皮間葉相互作用 / 細胞形質 / 遺伝子ネットワーク |
|---|
| Research Abstract |
We aimed to characterize distinct spatio-temporal expression patterns at the fusing epithelium and in the underlying mesenchyme of the mandibular arches and of the secondary palatal shelves in embryonic mice. To this end, we dissected out of the medial/lateral regions of mandibular arches at embryonic days 9.7-11.5(E9.7-11.5) and of the secondary palatal shelves at E14.0-14.5. The gene expression profiles of each of the tissues were determined using microarray and real-time quantitative PCR, together with immunohistochemical validation. Comparison of the spatio-temporal gene expression profiles between mandibular arches and secondary palatal shelves delineated that Igf family molecules may regulate mandibular fusion in the mandibular medial region, while both Cask and TgfB3 play essential roles in the contact and fusion between the bilateral shelves.
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