| Project/Area Number |
21592392
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathobiological dentistry/Dental radiology
|
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| Research Institution | Nagasaki University |
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Principal Investigator |
SHIBATA Yasuaki 長崎大学, 大学院・医歯薬学総合研究科, 助教 (80253673)
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| Co-Investigator(Kenkyū-buntansha) |
菱川 善隆 長崎大学, 医歯(薬)学総合研究科, 准教授 (60304276)
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| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 胸腺 / AIRE / デキサメタゾン / 胸腺上皮細胞 |
|---|
| Research Abstract |
Dexamethasone(Dex) induced trans-location of AIRE protein from nucleus to cytoplasm, and reduced expression of Aire, CD80 and Ins1 mRNA in thyms epithelial cells(TEC) of Fatal Thymus Organ culture(FTOC). The trans-location and reduced expression of these mRNAs were detected neither in mouse TEC culture system nor mouse thymus in vivo. These results indicated that translocation of nuclear Aire to cytoplasm inhibit differentiation of TEC, although the phenomenon were specific event in TEC of FTOC. In Sjogren' s syndrome(SS) mouse model, expression of Aire mRNA was totally reduced, however, the expression intensity depends on each lymph node. This result suggested that reduction of AIRE associated with mouse SS developing mechanism.
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