Epithelotropism of effector cells in immune disorders of the oral mucosa

• Project/Area Number: 21592406
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Fukuoka Dental College
• Principal Investigator: OHNO Jun 福岡歯科大学, 歯学部, 講師 (10152208)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 口腔粘膜疾患 / 免疫応答 / 上皮指向性 / エフェクター細胞 / satellitosis / サイトカイン / ケモカイン / lupus型粘膜炎 / CD8陽性T細胞 / リンパ球 / CD8

Research Abstract

Epithelotropism of effector cells were studied in rat models of satellitosis-induced and lichenoid oral mucosal disorders. In the satellitosis-induced oral lesion, the induction of ICAM-1 expression on epithelial keratinocytes leads to the migration of CD8+cells into the epithelium. The lichenoid lesion is characterized by intense infiltration of class II+and ED1+cells around the epithelial basement membrane showing deposition of autoantibodies. These findings provided insights into the pathophysiological roles of effector cells in oral mucosal lesions.

Research Products

• [Journal Article] Rat calvarial tissue response to flowable DNA/protamine complex mixtures with DNA/chitosan complex to be used as a protective membrane for guided bone regeneration (2011)
  • Author(s): Shinozaki Y, Mori N, Ohno J, Kawaguchi M, Kido H, Hayakawa T, Fukushima T
  • Journal Title: J Oral Tissue Engin Volume: 9 Pages: 159-166
  • NAID: 130004941181
  • Peer Reviewed
• [Journal Article] DNA/protamine complex paste for an injectable dental material (2011)
  • Author(s): Fukushima T, Ohno J, Imayoshi R, Mori N, Sakagami R, Mitarai M, Hayakawa T
  • Journal Title: J Mater Sci Mater Med Volume: 22 Pages: 2607-2615
  • Peer Reviewed
• [Journal Article] Computational approach for calcium complex formation of aromatic carboxylic acid monomers (2011)
  • Author(s): Hayakawa T, Hirayama S, Ohno J, Fukushima T
  • Journal Title: Nano Biomedicine Volume: 3 Pages: 200-207
  • NAID: 130004960255
  • Peer Reviewed
• [Journal Article] Cell viability and tissue response of high molecule weight DNA/protamine complex (2011)
  • Author(s): Mori N, Iwahashi T, Ohno J, Shinozaki Y, Sakagami R, Mitarai M, Fukushima T
  • Volume: 8
  • Pages: 188-194
  • NAID: 130004456620
  • Peer Reviewed
• [Journal Article] Dispersion stability and exothermic properties of DNA-functionalized single-walled carbon nanotubes (2011)
  • Author(s): Kawaguchi M, Ohno J, Irie A, Fukushima T, Yamazaki J, Nakashima N
  • Journal Title: Int J Nanomedicine Volume: 6 Pages: 729-736
  • Peer Reviewed
• [Journal Article] Induction of epitheial migration of lymphocytes by intercellular adhesion molecule-1 in a rat model of oral mucosal graft-versus-host disease (2011)
  • Author(s): Ohno J, Iwahashi T, Ehara M, Ozasa R, Hanada H, Funakoshi T, Taniguchi K
  • Volume: 26
  • Pages: 725-733
  • Peer Reviewed
• [Journal Article] Alterations in PNA binding of keratinocytes in oral keratosis (2011)
  • Author(s): Ohno J, Iwahashi T, Ehara M, Taniguchi K
  • Journal Title: Biotech Histochem Volume: 86 Pages: 168-173
  • Peer Reviewed
• [Journal Article] Computational approach for calcium complex formation of aromatic carboxylic acid monomers (2011)
  • Author(s): Hayakawa T, et al
  • Journal Title: Nano Biomed Volume: 3 Pages: 200-207
  • Peer Reviewed
• [Journal Article] Cell viability and tissue response of high molecular weight DNA/protamine complex (2011)
  • Author(s): Mori, et al
  • Journal Title: J Oral Tissue Engin Volume: 8 Pages: 188-194
  • Peer Reviewed
• [Journal Article] Induction of epithelial migration of lymphocytes by intercellular adhesion molecule-1 in a rat model of oral mucosal graft-versus-host disease (2011)
  • Author(s): Ohno J, et al
  • Journal Title: Histol Histopathol Volume: 26 Pages: 725-733
  • Peer Reviewed
• [Journal Article] Soliatry neurofibroma of the gingiva with prominent differentiation of Meissner bodies : a case report (2010)
  • Author(s): Ohno J, Iwahashi T, Ozasa R, Okamura K, Taniguchi K
  • Journal Title: Diagn Pathol Volume: 22 Pages: 61-61
  • Peer Reviewed
• [Journal Article] Polycaationic protamine for water-insoluble complex formation with DNA (2010)
  • Author(s): Fukushima T, Ohno J, Hayakawa T, Imayoshi R, Kawagushi M, Doi Y, Kanaya K, Mitarai M
  • Journal Title: Dent Mater J Volume: 29 Pages: 529-535
  • Peer Reviewed
• [Journal Article] A poly(gamma-glutamic acid)-amphiphile complex as a novel nanovehicle for drug delivery system (2010)
  • Author(s): Akao T, Kimura T, Hirofuji YS, Matsunaga K, Imayoshi R, Nagao J, Cho T, Kaminishi H, Ohatono S, Ohno J, Taniguchi K
  • Journal Title: J Drug Target Volume: 18 Pages: 550-556
  • Peer Reviewed
• [Journal Article] Solitary neurofibroma of the gingiva with prominent differentiation of Meissner bodies : a case report (2010)
  • Author(s): Ohno J, et al
  • Journal Title: Diagn Pathol Volume: 5 Pages: 61-61
  • Peer Reviewed
• [Journal Article] Alterations in PNA binding of keratinocytes in oral keratosis (2010)
  • Author(s): Ohno, J, et al
  • Journal Title: Biotech Histochem. 85(in press)
  • Peer Reviewed
• [Journal Article] A poly (gamma-glutamic acid)-amphiphile complex as a novel nanovehicle for drug delivery system (2010)
  • Author(s): Akao T, et al
  • Journal Title: J Drug Target (in press)
  • Peer Reviewed
• [Journal Article] Bone response of DNA-chitosan-apatite complexes (2009)
  • Author(s): Kawaguchi M, Ohno J, IwahashiT, Fukushima T, Hayakawa T, Doi Y
  • Journal Title: J Oral Tissue Engin Volume: 7 Pages: 89-98
  • NAID: 130004456610
  • Peer Reviewed
• [Journal Article] Mold fabrication and biological assessment of porous DNA-chitosan complexed (2009)
  • Author(s): Fukushima T, Ohno J, Hayakawa T, Kawaguchi M, Inoue Y, Takeda S, Toyoda M, Okahata Y
  • Journal Title: J Biomed Mater Res B Appl Biomater Volume: 91 Pages: 746-754
  • Peer Reviewed
• [Journal Article] Giant cell fibroma of the tongue : report of a case showing unique S-100 protein and HLA-DR immunolocalization with literature review (2009)
  • Author(s): Okamura K, Ohno J, Iwahashi T, Enoki N, Taniguchi K, Yamazaki J
  • Journal Title: Oral Med Pathol Volume: 13 Pages: 75-79
  • NAID: 130004509522
  • Peer Reviewed
• [Journal Article] Mold fabrication and biological assessment of porous DNA-chitosan complexes (2009)
  • Author(s): Fukushima T, et al
  • Journal Title: J Biomed Mater Res B Appl Biomater 91B Pages: 746-754
  • Peer Reviewed
• [Presentation] ミニ移植法によるマウス口腔メラノーマ抑制の可能性 (2011)
  • Author(s): 大野純, 他
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2011-04-29
• [Presentation] ミニ移植法によるマウス口腔メラノーマ抑制の可能性 (2011)
  • Author(s): 大野純、岩橋輝明、谷口邦久
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 東京
• [Presentation] サケ白子由来DNA/プロタミン複合体の骨形成能 (2011)
  • Author(s): 大野純、森南奈、岩橋輝明、御手洗誠、庵原啓治、早川徹、福島忠男
  • Organizer: 第10回日本再生医療学会
  • Place of Presentation: 東京
• [Presentation] ナノカーボン分子ヒーターの創製-CNT/抗体複合体の調整 (2010)
  • Author(s): 川口稔、入江明仁、福島忠男、大野純、山崎純、松浦正朗
  • Organizer: 第55回日本歯科理工学会
  • Place of Presentation: 東京
  • Year and Date: 2010-04-18
• [Presentation] 横紋筋侵入をともなう顆粒細胞腫の免疫組織化学的検討 (2010)
  • Author(s): 岩橋輝明、大野純、小笹竜起、榎規雄、岡村和彦、谷口邦久
  • Organizer: 第21回日本臨床口腔病理学会総会
  • Place of Presentation: 大阪
• [Presentation] センチネルリンパ節における腫瘍反応性リンパ節症の病態 (2010)
  • Author(s): 岩橋輝明、大野純、小笹竜起、谷口邦久
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京
• [Presentation] 口腔扁平上皮癌細胞のスフェロイド形成能 (2010)
  • Author(s): 大野純、岩橋輝明、谷口邦久
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京
• [Presentation] 骨形成促進効果を有するサケ白子由来DNAとプロタミンからなる複合体ペースト (2010)
  • Author(s): 森南奈、大野純、福島忠男、坂上竜資
  • Organizer: 第56回日本歯科理工学会
  • Place of Presentation: 岐阜
• [Presentation] マウス自然発症転移モデルにおけるセンチネルリンパ節の動態 (2009)
  • Author(s): 大野純、岩橋輝明、榎規雄、岡村和彦、谷口邦久
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 京都