The regulation of cell proliferation and adhesion by chloride channel related factor CLCA.
| Project/Area Number |
21592407
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Fukuoka Dental College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YAMAZAKI Jun 福岡歯科大学, 歯学部, 教授 (50230397)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | イオンチャネル / CLCA / 細胞増殖 / 分化 / 癌の浸潤 / クロライドチャネル / 発生・分化 / 細胞接着 / siRNA抑制 / 再構築モデル / p63 |
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| Research Abstract |
A rat CLCA(originally named chloride channels, calcium-activated) homologue fCLCA has a splicing isoform trCLCA. Owing to the weak association of trCLCA to the other CLCA isoforms of other species, the morphological and functional significance of trCLCA has not been established. We have carried out the tissue localization of trCLCA in rat, with support of grants-in-aid for Scientific Research(C) 18592025.Subsequently, functional analyses of trCLCA was undertaken in this project, using in vitro system of reconstruction model of stratified squamous epithelium and several kinds of cultured cells. One of important result is : inhibition of trCLCA expression by siRNA bring out the change of cytokeratin profile, followed by negative regulation of cellular adhesion and proliferation.
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Report
(4 results)
Research Products
(11 results)
