A study on the mechanisms of tissue repair and regeneration of dentin-pulp complex

• Project/Area Number: 21592417
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Niigata University
• Principal Investigator: YOSHIBA Kunihiko 新潟大学, 医歯学系, 准教授 (30220718)
• Co-Investigator(Kenkyū-buntansha): YOSHIBA Nagako 新潟大学, 医歯学総合病院, 講師 (10323974) ; SHIGETANI Yoshimi 新潟大学, 医歯学系, 助教 (80397132)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 象牙質・歯髄複合体 / 修復・再生 / 歯髄組織幹細胞 / 象牙芽細胞 / 組織培養 / Thy-1 / 覆髄材 / MTA / 非コラーゲン性タンパク / TR-PCR / 組織培養法 / αSMA / 半導体レーザー

Research Abstract

The aim of this study was to elucidate cellular events during pulp tissue repair and reparative dentin formation after tooth injury. The up-regulation ofα-smooth muscle actin(SMA) was characteristically observed in fibroblastic cells in an organ culture system of rat molar. Thy-1-positive dental pulp cells localized in the subodontoblastic layer had the ability to differentiate into hard tissue-forming cells. Theα-SMA-positive and/or Thy-1-positive cells may play a role in tissue repair and regeneration of dental pulp.

Research Products

• [Journal Article] Expressionalal terations of fibrilln-1 during wound healing of human dental pulp (2012)
  • Author(s): Yoshiba N, Yoshiba K, Ohkura N, Hosoya A, Shigetani Y, Yamanaka Y, Izumi N, Nakamura H, Okiji T
  • Journal Title: Journal of Endodontics Volume: 38(2) Pages: 177-184
  • Peer Reviewed
• [Journal Article] MTAの理化学的・生物学的特性と臨床 (2012)
  • Author(s): 興地隆史, 韓臨麟, 重谷佳見, 吉羽邦彦
  • Journal Title: 日本歯内療法学会雑誌 Volume: 33(1) Pages: 3-13
  • NAID: 10030283517
  • Peer Reviewed
• [Journal Article] MTAの理化学的・生物学的特性と臨床 (2012)
  • Author(s): 興地隆史
  • Journal Title: 日本歯内療法学会雑誌 Volume: 33 Pages: 3-13
  • Peer Reviewed
• [Journal Article] Expressional alterations of fibrillin-1 during wound healing of human dental pulp (2012)
  • Author(s): Yoshiba N, et al
  • Journal Title: J Endodont Volume: 37 Issue: 2 Pages: 177-184
  • DOI: 10.1016/j.joen.2011.09.016
  • Peer Reviewed
• [Journal Article] Immunohistochemical analysis of subcutaneous tissue reactions to methacrylate resin-based root canal sealers (2011)
  • Author(s): Yamanaka Y, Shigetani Y, Yoshiba K, Yoshiba N, Okiji T
  • Journal Title: International Endodontic Journal Volume: 44(7) Pages: 669-675
  • Peer Reviewed
• [Journal Article] GaAlAs laser irradiation induces active tertiary dentin formation after pulpal apoptosis and cell proliferation in rat molars (2011)
  • Author(s): Shigetani Y, Sasa N, Suzuki H, Okiji T, Ohshima H
  • Journal Title: Journal of Endodontics Volume: 37(8) Pages: 1086-1091
  • Peer Reviewed
• [Journal Article] GaAlAs laser irradiation induces active tertiary dentin formation after pulpal apoptosis and cell proliferation in rat molars (2011)
  • Author(s): Yoshimi Shigetani
  • Journal Title: Journal of Endodontics Volume: 37 Issue: 8 Pages: 1086-1091
  • DOI: 10.1016/j.joen.2011.05.020
  • Peer Reviewed
• [Journal Article] Immunohistochemical analysis of subcutaneous tissue reactions to methacrylate resin-based root canal sealers (2011)
  • Author(s): Yusuke Yamanaka
  • Journal Title: International Endodontic Journal Volume: 44 Issue: 7 Pages: 669-675
  • DOI: 10.1111/j.1365-2591.2011.01874.x
  • Peer Reviewed
• [Journal Article] Potential of periodontal ligament cells to regenerate alveolar bone (2010)
  • Author(s): Hosoya A, Ninomiya T, Hiraga T, Yoshiba K, Yoshiba N, Kasahara E, Ozawa H, Nakamura H
  • Journal Title: Journal of Oral Biosciences Volume: 52(2) Pages: 72-80
  • NAID: 10027105411
  • Peer Reviewed
• [Journal Article] 半導体レーザー照射後のラット臼歯における非コラーゲンタンパクの遺伝子発現 (2010)
  • Author(s): 重谷佳見, 大倉直人, 吉羽邦彦, 吉羽永子, 大島勇人, 興地隆史
  • Journal Title: 日本歯科保存学雑誌 Volume: 53(5) Pages: 495-501
  • NAID: 110008006920
  • Peer Reviewed
• [Journal Article] 半導体レーザー照射後のラット臼歯における非コラーゲンタンパクの遺伝子発現 (2010)
  • Author(s): 重谷佳見
  • Journal Title: 日本歯科保存学雑誌 Volume: 53 Pages: 495-501
  • NAID: 110008006920
  • Peer Reviewed
• [Journal Article] Mineraltrioxide aggregate(MTA)の物理化学的特性と直接覆髄後の歯髄反応 (2009)
  • Author(s): 吉羽邦彦, 鞍立桃子, 重谷佳見, 韓臨麟, 吉羽永子, 興地隆史
  • Journal Title: 新潟歯学会雑誌 Volume: 39(2) Pages: 181-182
  • Peer Reviewed
• [Journal Article] Reparative dentinogenesis induced by mineral trioxide aggregate : a review from the biological and physicochemical points of view (2009)
  • Author(s): Okiji T, Yoshiba K
  • Journal Title: International Journal of Dentistry Volume: vol.2009 Pages: 12-12
  • Peer Reviewed
• [Journal Article] Reparative dentinogenesis induced by mineral trioxide aggregate : a review from the biological and physicochemical points of view. (2009)
  • Author(s): Takashi Okiji
  • Journal Title: International journal of Dentistry Vol.2009
  • Peer Reviewed
• [Journal Article] Mineral trioxide aggregate(MTA)の物理化学的特性と直接覆髄後の歯髄反応 (2009)
  • Author(s): 吉羽邦彦
  • Journal Title: 新潟歯学会雑誌 39 Pages: 181-182
• [Presentation] Mineral Trioxide Aggregateの生体内組成変化:ラット皮下移植による検討 (2011)
  • Author(s): 吉羽邦彦,重谷佳見,山中裕介,武井絵梨花,吉羽永子,興地隆史
  • Organizer: 日本歯科保存学会2011年度季学術大会(第135回)
  • Place of Presentation: 大阪
  • Year and Date: 2011-10-20
• [Presentation] 水酸化カルシウム直接覆髄後の被蓋硬組織形成過程-ラット臼歯における免疫組織化学的研究- (2011)
  • Author(s): 重谷佳見,吉羽邦彦,鞍立桃子,吉羽永子,山中裕介,興地隆史
  • Organizer: 日本歯科保存学会2011年度季学術大会(第135回)
  • Place of Presentation: 大阪
  • Year and Date: 2011-10-20
• [Presentation] Mineral Trioxide Aggregateの生体内組成変化:ラット皮下移植による検討 (2011)
  • Author(s): 吉羽邦彦
  • Organizer: 日本歯科保存学会2011年度秋季学術大会(第135回)
  • Place of Presentation: 大阪国際交流センター(大阪市)
  • Year and Date: 2011-10-20
• [Presentation] 水酸化カルシウム直接覆髄後の被蓋硬組織形成過程-ラット臼歯における免疫組織化学的研究- (2011)
  • Author(s): 重谷佳見
  • Organizer: 日本歯科保存学会2011年度秋季学術大会(第135回)
  • Place of Presentation: 大阪国際交流センター(大阪市)
  • Year and Date: 2011-10-20
• [Presentation] Compositional changes of mineral trioxide aggregate subcutaneously implanted in rats (2011)
  • Author(s): Yoshiba K, Shigetani Y, Yamanaka Y, Takei E, Yoshiba N, Okiji T
  • Organizer: 59th Annual Meeting of JADR
  • Place of Presentation: 広島
  • Year and Date: 2011-10-08
• [Presentation] Expression of alpha-smooth muscle actin in dental pulp wound healing (2011)
  • Author(s): oshiba N, Yoshiba K, Ohkura N, Shigetani Y, Hosoya A, Nakamura H, Okiji T
  • Organizer: 59th Annual Meeting of JADR
  • Place of Presentation: 広島
  • Year and Date: 2011-10-08
• [Presentation] Compositional changes of mineral trioxide aggregate subcutaneously implanted in rats (2011)
  • Author(s): Kunihiko Yoshiba
  • Organizer: 59th Annual Meeting of Japanese Association of Dental Research
  • Place of Presentation: 広島国際会議場(広島市)
  • Year and Date: 2011-10-08
• [Presentation] Expression of alpha-smooth muscle actin in dental pulp wound healing (2011)
  • Author(s): Nagako Yoshiba
  • Organizer: 59th Annual Meeting of Japanese Association of Dental Research
  • Place of Presentation: 広島国際会議場(広島市)
  • Year and Date: 2011-10-08
• [Presentation] Thy-1陽性subodontoblastic layer細胞の高い硬組織形成能 (2011)
  • Author(s): 細矢明宏,平賀徹,二宮禎,雪田聡,吉羽邦彦,吉羽永子,中村浩彰
  • Organizer: 第9回日本再生歯科医学会学術大会
  • Place of Presentation: 大阪
  • Year and Date: 2011-09-10
• [Presentation] Thy-1陽性subodontoblastic layer細胞の高い硬組織形成能 (2011)
  • Author(s): 細矢明宏
  • Organizer: 第9回日本再生歯科医学会学術大会
  • Place of Presentation: 大阪国際会議場(大阪市)
  • Year and Date: 2011-09-10
• [Presentation] Mineral Trioxide Aggregateのラット皮下移植後の組成変化 (2011)
  • Author(s): 吉羽邦彦,重谷佳見,山中裕介,武井絵梨花,吉羽永子,興地隆史
  • Organizer: 第32回日本歯内療法学会学術大会
  • Place of Presentation: 長崎
  • Year and Date: 2011-07-31
• [Presentation] Mineral Trioxide Aggregateのラット皮下移植後の組成変化 (2011)
  • Author(s): 吉羽邦彦
  • Organizer: 第32回日本歯内療法学会学術大会
  • Place of Presentation: 長崎全日空ホテル(長崎市)
  • Year and Date: 2011-07-17
• [Presentation] Dentin sialophosphoprotein expression in engineered dentin pulp-like tissue (2010)
  • Author(s): Kanako T, Yamanaka Y, Yoshiba K, Yoshiba N, Suda H, Okiji T
  • Organizer: 58th Annual Meeting of JADR
  • Place of Presentation: 北九州
  • Year and Date: 2010-11-20
• [Presentation] Dentin sialophosphoprotein expression in engineered dentin pulp like tissue (2010)
  • Author(s): Tomoatsu Kanako
  • Organizer: 58th Annual Meeting of Japanese Association forDental Research
  • Place of Presentation: 九州歯科大学(北九州市)
  • Year and Date: 2010-11-20
• [Presentation] Thy-1陽性歯髄細胞の局在および硬組織形成能 (2010)
  • Author(s): 細矢明宏
  • Organizer: 日本歯科保存学会2010年度秋季学術大会(第133回)
  • Place of Presentation: 長良川国際会議場(岐阜)
  • Year and Date: 2010-10-28
• [Presentation] Temporal changes in mRNA expression of mineralized tissue matrix proteins in GaAlAs laser irradiated rat molars (2010)
  • Author(s): Shigetani Y, Ohkura N, Hosoya A, Yoshiba K, Yoshiba N, Ohshima H, Okiji T
  • Organizer: 8th World Endodontic Congress of IFEA
  • Place of Presentation: Athens(Greece)
  • Year and Date: 2010-10-08
• [Presentation] Temporal changes in mRNA expression of mineralized tissue matrix proteins in GaAIAs laser irradiated rat molars (2010)
  • Author(s): Yoshimi Shigetani
  • Organizer: 8th World Endodontic Congress of IFEA
  • Place of Presentation: アテネ(ギリシャ)
  • Year and Date: 2010-10-08
• [Presentation] Tissue alteration of rat dental pulp in whole tooth culture (2010)
  • Author(s): Yoshiba K, Yoshiba N, Shigetani Y, Hosoya A, Okiji T
  • Organizer: 88th General Session & Exhibition of the IADR
  • Place of Presentation: Barcelona(Spain)
  • Year and Date: 2010-07-15
• [Presentation] Expressional alterations of fibrillin-1 during wound healing of dental pulps (2010)
  • Author(s): Yoshiba N, Yoshiba K, Ohkura N, Hosoya A, Shigetani Y, Okiji T
  • Organizer: 88th General Session & Exhibition of the IADR
  • Place of Presentation: Barcelona(Spain)
  • Year and Date: 2010-07-15
• [Presentation] Tissue alteration of rat dental pulp in whole tooth culture (2010)
  • Author(s): Kunihiko Yoshiba
  • Organizer: 88th General Session & Exhibition of the IADR
  • Place of Presentation: バルセロナ(スペイン)
  • Year and Date: 2010-07-15
• [Presentation] Expressional alterations of fibrillin-1 during wound healing of dental pulps (2010)
  • Author(s): Nagako Yoshiba
  • Organizer: 88th General Session & Exhibition of the IADR
  • Place of Presentation: バルセロナ(スペイン)
  • Year and Date: 2010-07-15
• [Presentation] ヒト歯髄創傷治癒過程で生じるFibrillin-1の分解は細胞分化と石灰化を誘導する (2010)
  • Author(s): 吉羽永子,吉羽邦彦,大倉直人,細矢明宏,重谷佳見,興地隆史
  • Organizer: 日本歯科保存学会2010年度春季学術大会(第132回)
  • Place of Presentation: 熊本
  • Year and Date: 2010-06-04
• [Presentation] ヒト歯髄創傷治癒過程で生じるFibrillin-1の分解は細胞分化と石灰化を誘導する (2010)
  • Author(s): 吉羽永子
  • Organizer: 日本歯科保存学会2010年度春季学術大会(第132回)
  • Place of Presentation: 熊本市民会館(熊本)
  • Year and Date: 2010-06-04
• [Presentation] Gene Expression of Mineralized Tissue Matrix Proteins in GaAlAs Laser-irradiated Rat Molars (2010)
  • Author(s): Shigetani Y, Ohkura N, Sasa N, Yoshiba K, Yoshiba N, Okiji T
  • Organizer: The 8th Joint Scientific Meeting between JEA and KAE
  • Place of Presentation: Busan(Korea)
  • Year and Date: 2010-03-27
• [Presentation] Gene expression of mineralized tissue matrix proteins in GaAlAs laserirradiated rat molars. (2010)
  • Author(s): Yoshimi Shigetani
  • Organizer: The 8th Joint Scientific Meeting between JEA and KAE
  • Place of Presentation: 韓国
  • Year and Date: 2010-03-27
• [Presentation] ラット臼歯培養系における歯髄組織の変化に関する免疫組織化学的観察 (2010)
  • Author(s): 吉羽邦彦,吉羽永子,重谷佳見,金子友厚,細矢明宏,興地隆史
  • Organizer: 日本歯科保存学会2010年度秋季学術大会
  • Place of Presentation: 岐阜
• [Presentation] 半導体レーザー照射後のラット臼歯における非コラーゲンタンパクの遺伝子発現 (2010)
  • Author(s): 重谷佳見,大倉直人,吉羽邦彦,吉羽永子,細矢明宏,興地隆史
  • Organizer: 日本歯科保存学会2010年度秋季学術大会(第133回)
  • Place of Presentation: 岐阜
• [Presentation] Thy-1陽性歯髄細胞の局在および硬組織形成能 (2010)
  • Author(s): 細矢明宏,吉羽邦彦,吉羽永子,笠原悦男,中村浩彰
  • Organizer: 日本歯科保存学会2010年度秋季学術大会(第133回)
  • Place of Presentation: 岐阜
• [Presentation] ラット炎症歯髄に対する薬物輸送担体の遺伝子発現解析 (2010)
  • Author(s): 大倉直人,重谷佳見,吉羽永子,吉羽邦彦,細矢明宏,興地隆史
  • Organizer: 日本歯科保存学会2010年度秋季学術大会(第133回)
  • Place of Presentation: 岐阜
• [Presentation] ヒト歯髄組織においてFibrillin-1はMMP-3により分解され細胞分化と石灰化を誘導する (2010)
  • Author(s): 吉羽永子,吉羽邦彦,重谷佳見,興地隆史,細矢明宏,中村浩彰
  • Organizer: 第52回歯科基礎医学会学術大会
  • Place of Presentation: 東京
• [Presentation] Thy-1陽性歯髄細胞の硬組織形成能に関する形態学的研究 (2010)
  • Author(s): 細矢明宏,平賀徹,二宮禎,雪田聡,吉羽邦彦,吉羽永子,中村浩彰
  • Organizer: 第52回歯科基礎医学会学術大会
  • Place of Presentation: 東京
• [Presentation] 半導体レーザー照射後のラット臼歯における硬組織関連タンパクの遺伝子発現 (2010)
  • Author(s): 重谷佳見,大倉直人,吉羽邦彦,吉羽永子,興地隆史
  • Organizer: 第31回日本歯内療法学会学術大会
  • Place of Presentation: 東京
• [Presentation] 半導体レーザー照射後のラット臼歯における硬組織関連タンパクの遺伝子発現 (2010)
  • Author(s): 重谷佳見
  • Organizer: 第31回日本歯内療法学会学術大会
  • Place of Presentation: 東商ホール(東京都)
• [Presentation] Thy-1陽性歯髄細胞の硬組織形成能に関する形態学的研究 (2010)
  • Author(s): 細矢明宏
  • Organizer: 第52回歯科基礎医学会学術大会
  • Place of Presentation: タワーホール船堀(東京都)
• [Presentation] ヒト歯髄組織においてFibrillin-1はMMP-3により分解され細胞分化と石灰化を誘導する (2010)
  • Author(s): 吉羽永子
  • Organizer: 第52回歯科基礎医学会学術大会
  • Place of Presentation: タワーホール船堀(東京都)
• [Presentation] ラット炎症歯髄に対する薬物輸送担体の遺伝子発現解析 (2010)
  • Author(s): 大倉直人
  • Organizer: 日本歯科保存学会2010年度秋季学術大会(第133回)
  • Place of Presentation: 長良川国際会議場(岐阜)
• [Presentation] ラット臼歯培養系における歯髄組織の変化に関する免疫組織化学的観察 (2010)
  • Author(s): 吉羽邦彦
  • Organizer: 日本歯科保存学会2010年度秋季学術大会(第133回)
  • Place of Presentation: 長良川国際会議場(岐阜)
• [Presentation] 半導体レーザー照射後のラット臼歯における非コラーゲンタンパクの遺伝子発現 (2010)
  • Author(s): 重谷佳見
  • Organizer: 日本歯科保存学会2010年度秋季学術大会(第133回)
  • Place of Presentation: 長良川国際会議場(岐阜)
• [Presentation] Immunolocalization of osteopontin and dentin matrix protein-1 during reparative dentinogenesis in rat molars after direct pulp capping with calcium hydroxide (2009)
  • Author(s): Kuratate M, Yoshiba K, Shigetani Y, Yoshiba N, Okiji T
  • Organizer: The 11th Joint Meeting between Korean Academy of Conservative Dentistry and Japanese Society of Conservative Dentistry
  • Place of Presentation: Jeju(Korea)
  • Year and Date: 2009-11-13
• [Presentation] Immunolocalization of osteopontin and dentin matrix protein-1 during reparative dentinogenesis in rat molars after direct pulp capping with calcium hydroxide. (2009)
  • Author(s): Momoko Kuratate
  • Organizer: The 11th Joint Meeting between Korean Academy of Coneservative Dentistry and Japanese Society of Conservative Dentistry
  • Place of Presentation: 韓国
  • Year and Date: 2009-11-13
• [Presentation] 半導体レーザー照射後のラット臼歯歯髄反応に関する免疫組織学的検討 (2009)
  • Author(s): 笹なつき
  • Organizer: 日本歯科保存学会2009年度秋季学術大会(第131回)
  • Place of Presentation: 仙台
  • Year and Date: 2009-10-29
• [Presentation] ヒト歯髄組織におけるFibrillin-1の分解が細胞分化および石灰化に及ぼす影響 (2009)
  • Author(s): 吉羽永子
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 新潟
  • Year and Date: 2009-09-11
• [Presentation] 半導体レーザー照射に対するラット臼歯歯髄初期反応 (2009)
  • Author(s): 笹なつき
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 新潟
  • Year and Date: 2009-09-10
• [Presentation] ヒト歯髄組織におけるFibrillin-1の局在に関する免疫組織化学的研究-石灰化および細胞分化との関連性について- (2009)
  • Author(s): 吉羽永子
  • Organizer: 日本歯科保存学会2009年度春季学術大会(第130回)
  • Place of Presentation: 札幌
  • Year and Date: 2009-06-12
• [Presentation] 水酸化カルシウム直接覆髄後の硬組織形成過程-ラット臼歯を用いた免疫組織化学的研究- (2009)
  • Author(s): 鞍立桃子
  • Organizer: 日本歯科保存学会2009年度春季学術大会(第130回)
  • Place of Presentation: 札幌
  • Year and Date: 2009-06-11
• [Presentation] Early pulpal responses to GaAlAs laser irradiation in rat molars. (2009)
  • Author(s): Yoshimi Shigetani
  • Organizer: 15th Scientific Congress of the Asian Pacific Endodontic Confederation
  • Place of Presentation: 東京
  • Year and Date: 2009-04-25