The analysis of function and localization of matrixmetalloproteinase in the process of forming tertiary dentin.

• Project/Area Number: 21592419
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Osaka University
• Principal Investigator: ITOH Shousaku 大阪大学, 大学院・歯学研究科, 助教 (90360495)
• Co-Investigator(Kenkyū-buntansha): YABUNE Toshiaki 大阪大学, 大学院・歯学研究科, 助教 (90423144) ; ABE Makoto 大阪大学, 大学院・歯学研究科, 助教 (40448105) ; TAKAHASI Yusuke 大阪大学, 大学院・歯学研究科, 助教 (60397693)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 修復象牙質 / MMPファミリー / in situ hydridization / MDPC-23 / 窩洞形成 / in situ hybridization / 骨芽細胞 / 石灰化 / 覆髄剤 / 硬組織産生細胞 / カルシウムセンシングレセプター / シグナル

Research Abstract

TIMP1, which promotes the mineralization of odontoblast and activates undifferentiated mesenchymal cells via Wnt/β-catenin pathway in the pulp after cavity preparation, might be one of a critical molecule for tertiary dentinogenesis and one of a candidate for a new" biological" pulp capping materials.

Research Products

• [Journal Article] Kruppel-like factor 4 regulates membranous and endochondral ossification (2012)
  • Author(s): Ikumi Michikami, Toshiya Fukushi, Mariko Tanaka, Hiroshi Egusa, Yoshinobu Maeda, Takashi Ooshima, Satoshi Wakisaka, Makoto Abe
  • Journal Title: EXPERIMENTAL CELL RESEARCH
  • Peer Reviewed
• [Journal Article] Kruppel-like factor 4 regulates membranous and endochondral ossification (2012)
  • Author(s): Michikami I, Fukushi T, Tanaka M, Egusa H, Maeda Y, Ooshima T, Wakisaka S, Abe M
  • Journal Title: Experimental Cell Research Volume: 318(4) Issue: 4 Pages: 311-325
  • DOI: 10.1016/j.yexcr.2011.12.013
  • Peer Reviewed
• [Journal Article] An explanation of the mineralization mechanism in osteoblasts induced by calcium hydroxide (2010)
  • Author(s): 成田寛子, 伊藤祥作
  • Journal Title: Acta Biomaterialia 6 Pages: 586-590
  • Peer Reviewed
• [Presentation] 新規幹細胞集団における硬組織再生能の評価 (2011)
  • Author(s): 伊藤祥作、松下健太、池田峻、山本由美子、恵比須繁之
  • Organizer: 第134回日本歯科保存学会秋季学術大会
  • Place of Presentation: 大阪
  • Year and Date: 2011-10-20
• [Presentation] 修復象牙質形成過程におけるTissue inhibitor of metalloprotease 1の発現誘導 (2011)
  • Author(s): 吉岡靖介、高橋雄介、今里聡、恵比須繁之
  • Organizer: 第134回日本歯科保存学会春季学術大会
  • Place of Presentation: 東京
  • Year and Date: 2011-06-09
• [Presentation] 骨髄ストローマ細胞の骨芽細胞分化におけるLIFの機能解析 (2011)
  • Author(s): 松下健太、伊藤祥作、池田峻、恵比須繁之
  • Organizer: 第134回日本歯科保存学会春季学術大会
  • Place of Presentation: 東京
  • Year and Date: 2011-06-09
• [Presentation] Effects of Calcium Hydroxide on Osteoblast Differentiation and Mineralization (2010)
  • Author(s): H. Nasa, S. Itoh, S. Imazato, S. Ebisu
  • Organizer: 第58回Japanese Association for Dental Research
  • Place of Presentation: 福岡
  • Year and Date: 2010-11-21
• [Presentation] 象牙質基質タンパク質分解産物が歯髄細胞に与える影響 (2010)
  • Author(s): 高橋雄介、吉岡靖介、今里聡、恵比須繁之
  • Organizer: 第133回日本歯科保存学会秋季学術大会
  • Place of Presentation: 岐阜
  • Year and Date: 2010-10-29
• [Presentation] 高度骨再生能を有する新規細胞集団精製法についての報告 (2010)
  • Author(s): 伊藤祥作、松下健太、恵比須繁之
  • Organizer: 第28回日本骨代謝学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-23
• [Presentation] 高度骨再生能を有する新規細胞集団精製法についての報告 (2009)
  • Author(s): 伊藤祥作
  • Organizer: 第131回 日本歯科保存学会
  • Place of Presentation: 仙台
  • Year and Date: 2009-10-29