Immune-histological observations of reconstructed gingiva-like cultures using epithelial cells derived from PDL

• Project/Area Number: 21592434
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: The Nippon Dental University
• Principal Investigator: IGARASHI Masaru 日本歯科大学, 新潟生命歯学部, 教授 (90168104)
• Co-Investigator(Kenkyū-buntansha): KITAJIMA Kayoko 日本歯科大学, 新潟生命歯学部, 准教授 (00177841) ; ARAI Kyoko 日本歯科大学, 新潟生命歯学部, 講師 (10434143)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 歯根膜 / 上皮細胞 / 線維芽細胞 / 再構成培養 / 免疫染色 / コラーゲンゲル / 分化 / 移植 / ブタ乳歯 / 免疫組織 / ヘルトウィッヒ上皮鞘 / サイトケラチン

Research Abstract

This investigation showed the comparison the differentiations and proliferations of the epithelial cells derived from periodontal ligaments or Hertwig's epithelial root sheath against to gingiva. The three dimensional organotypic cultures were incubated under air-liquid interface environment. The specimens showed that the multilayered epithelial structures without keratinization and the dispersing tendency of the cells in the epithelial layer were seen after 2 weeks incubation. The results showed that the reconstructed tissues were not similar to original gingiva. The continuous proper growth without scar after transplantation to immunodeficiency mice indicated the usefulness of transplantations.

Research Products

• [Journal Article] Cytokeratin expression of engrafted three-dimensional culture tissuesusing epithelial cells derived from porcine periodontal ligaments (2014)
  • Author(s): Yamada R, Kitajima K, Arai K, Igarashi M
  • Journal Title: J Oral Pathol MedJournal of Oral Pathology & Medicine Volume: 43 Issue: 8 Pages: 637-645
  • DOI: 10.1111/jop.12183
  • Peer Reviewed
• [Presentation] Three dimensional Culture of Epithelial Cells derived from Hertwig Epithelial Sheath (2012)
  • Author(s): Masaru IGARASHI, Kayoko KITAJIMA, Kyoko ARAI
  • Organizer: Japan China Dental Conference 2012
  • Place of Presentation: West China College of Stomatology, Sichuan University
  • Year and Date: 2012-04-27
• [Presentation] Histochemical observations of engrafted organotypic cultures using epithelial cells and fibroblasts derived from PDL (2012)
  • Author(s): Igarashi M, Kitajima K, Arai K, Yamada R
  • Organizer: The 10th JEA-KAE Joint Scientific Meeting
  • Place of Presentation: Seoul, SETEC
• [Presentation] Histological observations of engrafted organotypic cultures using epithelial cells and fibroblasts derived from PDL (2012)
  • Author(s): Igarashi Masaru, Kitajima Kayoko, Arai Kyoko, Yamada Rie
  • Organizer: The 10^<th> JEA-KAE Joint Scientific Meeting
  • Place of Presentation: Seoul, Korea SETEC
• [Presentation] ブタ乳歯歯根膜から得た上皮細胞を用いた三次元再構成培養組織の移植後における免疫組織学的研究 (2011)
  • Author(s): 山田理絵, 松田浩一郎, 新井恭子, 北島佳代子, 五十嵐勝
  • Organizer: 日本歯科保存学会2011年度秋季学術大会(第135回)プログラム及び講演抄録集
  • URL: http://wwwsoc.nii.ac.jp/jscd/member/pdf/abstract135/all.pdf
  • Place of Presentation: 大阪国際交流センター、大阪
  • Year and Date: 2011-10-20
• [Presentation] ブタ乳歯歯根膜から得た上皮細胞を用いた三次元再構成培養組織の移植後における免疫組織学的観察 (2011)
  • Author(s): 山田理絵、新井恭子、北島佳代子、松田浩一郎、五十嵐勝
  • Organizer: 第135回日本歯科保存学会
  • Place of Presentation: 大阪国際交流センター
  • Year and Date: 2011-10-19
• [Presentation] ヘルトウィッヒ上皮鞘から得られた上皮様細胞を用いた三次元培養の免疫組織学的観察 (2010)
  • Author(s): 五十嵐勝、北島佳代子、新井恭子、山田理絵、松田浩一郎
  • Organizer: 日本歯科保存学雑誌,秋季学術大会(第133回)プログラム及び講演抄録集
  • URL: http://wwwsoc.nii.ac.jp/jscd/member/pdf/abstract133/all.pdf
  • Place of Presentation: 長良川国際会議場.岐阜
  • Year and Date: 2010-10-28
• [Presentation] Three-dimensional Culture of Epithelial Cells derived from Hertwig Epithelial Sheath (2010)
  • Author(s): M.IGARASHI, K.KITAJIMA, K.ARAI
  • Organizer: The 88th General Session and Exhibition of IADR2010
  • Place of Presentation: スペイン、バルセロナ
  • Year and Date: 2010-07-15
• [Presentation] Three-dimensional Culture of Epithelial Cells derived from Hertwig Epithelial Sheath (2010)
  • Author(s): M. IGARASHI, K. KITAJIMA, and K. ARAI
  • Organizer: IADR
  • URL: http://www.dentalresearch.org
  • Place of Presentation: Barcelona, CCIB, Spain
• [Presentation] 歯根膜から得た上皮細胞と線維芽細胞を用いた三次元培養のkeratinizationに関する免疫組織学的観察 (2010)
  • Author(s): 北島佳代子、松田浩一郎、山田理絵、新井恭子、五十嵐勝
  • Organizer: 日本歯科保存学会2010年度春季保存学会学術大会(第132回)プログラムおよび講演抄録集
  • Place of Presentation: 崇城大学市民ホール、熊本
• [Presentation] Histochemical observations of the three-dimensional cultures using epithelial cells and fibroblasts derived from periodontal ligament (2010)
  • Author(s): Kayoko Kitajima, Kyoko Arai, Koichiro Matsuda, Rie Yamada, Masaru Igarashi
  • Organizer: プログラムおよび抄録集JEA & KAE Joint Endodontic Meeting
  • Place of Presentation: BEXCO, Busan, Korea
• [Presentation] 歯根膜から得た上皮細胞と線維芽細胞を用いた三次元培養に対する組織学的観察 (2009)
  • Author(s): 北島佳代子, 新井恭子, 長谷川有紀, 松田浩一郎, 山田理絵, 五十嵐勝
  • Organizer: 日本歯科保存学雑誌,秋季学術大会(第131回)プログラム及び講演抄録集
  • Place of Presentation: 仙台国際センター、仙台