Development of technical bases for biotooth formation by using iPS cells
| Project/Area Number |
21592500
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Dental engineering/Regenerative dentistry
|
|---|
| Research Institution | Iwate Medical University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
HARADA Hidemitsu 岩手医科大学, 歯学部, 教授 (70271210)
OTSU Keishi 岩手医科大学, 歯学部, ポストドクター (60509066)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | Biotooth / iPS細胞 / 歯の再生 |
|---|
| Research Abstract |
Induced pluripotent stem(iPS) cells are generated from somatic cells by the simultaneous introduction of several factors ; and they differentiate into the3embryonic germlayers with an extensive proliferative capacity. Recently, many researchers have reported that iPS cells can differentiate into different cell types, such as neurons, cardiac myocytes, and renal lineage cells, under appropriate conditions. Therefore, iPS cells have emerged as potential cell sources for tooth regeneration. In this study, we aimed to form biotooth by recombination of iPS cell-induced dental germ cells with mouse dental germ cells. First, we established the induction methods of iPS into mesenchymal stem cell(MSC). The differentiated cells expressed STRO-1, MSC marker, more than90%, as well as other MSC markers. Further, we established a culture protocol to induce the differentiation of iPS cells into neural crest like cells(NCLC), which is a precursor cell of dental mesenchymal cells. After the induction, the most of cells expressed neural crest cells markers. The cells did not form teratomas after transplantation into nude mice subcutaneously, suggesting the safety for clinical applications. Moreover, in recombination cultures of NCLC and mousedental epithelium, NCLC exhibited a gene expression pattern involving dental mesenchymal cells. Some NCLC also expressed dentin sialoprotein. Conditioned medium of mouse dental epithelium cultures further enhanced the differentiation of NCLC into odontoblasts. These results suggest that iPS cells are useful cell sources for tooth regeneration and tooth development studies.
|
Report
(4 results)
Research Products
(79 results)
-
[Journal Article] Differentiation of induced pluripotent stem cells into dental mesenchymal cells2012
Author(s)
Otsu, K., Kishigami, R., Oikawa-Sasaki, A., Fukumoto, S., Yamada, A., Fujiwara, N., Ishizeki, K. Harada, H.
-
Journal Title
Stem Cells & Dev. e-Pub
Volume: (in press)
Related Report
Peer Reviewed
-
-
[Journal Article] Role of epithelial-stem cell interactions during dental cell differentiation2012
Author(s)
Arakaki, M., Ishikawa, M., Nakamura, T., Iwamoto, T., Yamada, A., Fukumoto, E., Saito, M., Otsu, K., Harada, H., Yamada, Y., Fukumoto, S.
-
Journal Title
J. Biol. Chem
Volume: 287
Pages: 10590-10601
Related Report
Peer Reviewed
-
[Journal Article] Histological analysis of epithelial stem cells during induced pluripotent stem cell-derived teratoma development2012
Author(s)
Kishigami, R., Otsu, K., Oikawa-Sasaki, A., Fujiwara, N., Ishizeki, K., Tabata, Y., Harada, H.
-
Journal Title
J. Oral Biosci
Volume: 54
Pages: 54-5
Related Report
Peer Reviewed
-
-
-
-
[Journal Article] Hepatocyte growth factor stimulates root growth during the development of mouse molar teeth2011
Author(s)
Sakuraba, H., Fujiwara, N., Sasaki-Oikawa, A., Sakano, M., Otsu, K., Ishizeki, K., Harada, H.
-
Journal Title
J. Period. Res
Volume: 47
Pages: 81-88
Related Report
Peer Reviewed
-
[Journal Article] Establishment of Hertwig's epithelial root sheath cell line from cells involved in epithelial-mesenchymal transition2011
Author(s)
Akimoto, T., Fujiwara, N., Kagiya, T., Otsu, K., Ishizeki, K., Harada, H.
-
Journal Title
BBRC
Volume: 404
Pages: 308-312
Related Report
Peer Reviewed
-
-
-
-
-
-
[Journal Article] Establishment of Hertwig's epithelial root sheath cell line from cells involved in epithelial-mesenchymal transition2011
Author(s)
Akimoto, T., Fujiwara, N., Kagiya, T., Otsu.K., Ishizeki, K., Harada, H.
-
Journal Title
Biochem.Biophys.Res.Commun.
Volume: 404
Pages: 308-312
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Establishment of immortalized Hertwig's epithelial root sheath cells and character of cell line2010
Author(s)
Fujiwara, N., Akimoto, T., Sasaki-Oikawa, Ai., Otsu, K., Mayama H., Harada, H.
Organizer
The 10^<th> Tooth Morphogenesis and Differentiation
Place of Presentation
Berlin, Germany
Related Report
-
[Presentation] New hypothesis of cross-striation formation mechanism2010
Author(s)
Sasaki-Oikawa, Ai., Otsu, K., Fujiwara, Xu, L., Taniguchi, A., Harada, H.
Organizer
The 10^<th> Tooth Morphogenesis and Differentiation
Place of Presentation
Berlin, Germany
Related Report
-
-
[Presentation] Cell culture of human dental epithelium cells and its approach for regenerative dentistry2010
Author(s)
Mayama, H., Fujiwara, N., Sasaki-Oikawa, Ai., Otsu, K., Sakuraba, H., Miura, H., Harada, H.
Organizer
The 10^<th> Tooth Morphogenesis and Differentiation
Place of Presentation
Berlin, Germany
Related Report
-
[Presentation] Biological effects of cyclic diarylheptanoids on tooth root formation.2010
Author(s)
Lee, J.-W., Ota, M.S., Fujiwara, N., Yonezawa, T., Date, Y., Harada, H., Iseki, S., Seo, H.-J., Jeon, W.-B., Cha, B.-Y., Nagai, K., Woo, J.-T.
Organizer
The 10^<th> Tooth Morphogenesis and Differentiation
Place of Presentation
Berlin, Germany
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
