Pathological analysis of the deformed temporomandibular joint disc in epithelial-mesenchymal transition and fibrosis.
| Project/Area Number |
21592536
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TOJYO Itaru 和歌山県立医科大学, 医学部, 助教 (70405439)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 顎関節関節円板 / ルミカン / ファイブロモジュリン / 低分子ヒアルロン酸合成酵素 / TNF-α / 上皮-間葉移行 / α-smooth muscle actin / 関節円板 / HAS3 / デコリン / ビグリカン / α-smooth muscle action |
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| Research Abstract |
In this study, we showed that in immunohistochemistry, positive staining for lumican and hyaluronan synthase 3(HAS3) was observed in deformed temporomandibular joint(TMJ) disc, whereas fibromodulin expression was localized slightly below deformed TMJ disc surface. In cell culture experiment, the expression of lumican and HAS3 mRNA was significantly enhanced by stimulation with interleukin-1β(IL-1β), whereas there were no significant changes in the expression of fibromodulin mRNA in the presence or absence of IL-1β. In animal experiment, fibrous adhesion observed in the wild-type mice TMJ, whereas there was no fibrous adhesion in the tenascin-C knockout mice.
These findings suggest that to analyze the changes in ECM structure of TMJ disc may be important for understanding fibrous induction in EMT.
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Report
(4 results)
Research Products
(29 results)
