Identification of new predictive factors for chemo-radiation against oral cancers and setting of individualized medicine based on their expressions

• Project/Area Number: 21592555
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Yamaguchi University
• Principal Investigator: HARADA Koji 山口大学, 大学院・医学系研究科, 助教 (60253217)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 口腔癌 / 化学放射線療法 / 効果予測 / 耐性株 / 5-FU耐性 / 放射線耐性

Research Abstract

We established anticancer drug-resistant or radiation-resistant oral cancer cell lines. The specific fluctuating genes between a 5-FU-resistant cell line and a non 5-FU-resistant cell line were identified through microarray analysis, and we identified candidate genes including GDF15. We found a trifluorothymidine by investigating the agent can suppress the candidate gene expression. These finding suggested that trifluorothymidine based chemotherapy may be one of options for recurrent cases after 5-FU based chemotherapy.

Research Products

• [Journal Article] Enhancement of tumor radioresponse by combined treatment with Cepharanthine is accompanied by inhibition of DNA damage repair and induction of apoptosis in oral squamous cell carcinoma (2012)
  • Author(s): Toyoko Harada, Koji Harada, Yoshiya Ueyama
  • Journal Title: Int J Oncol Volume: (in press)
  • Peer Reviewed
• [Journal Article] 口腔扁平上皮癌における分子標的薬BortezomibとTS-1の併用効果についての基礎的研究 (2011)
  • Author(s): 原田豊子、原田耕志、上山吉哉
  • Journal Title: 頭頸部癌 Volume: 37巻 Pages: 536-541
  • NAID: 10030815616
  • Peer Reviewed
• [Journal Article] Comparison of apatite-coated titanium prepared by blast coating and flame spray methods-evaluation using simulated body fluid and initial histological study (2011)
  • Author(s): Takamitsu Mano, Kunio Ishikawa, Koji Harada, Hirotsugu Umeda, Yoshiya Ueyama
  • Journal Title: Dent Mater J Volume: 30 Pages: 431-437
  • NAID: 10029338467
  • Peer Reviewed
• [Journal Article] Migration-stimulating factor as a novel biomarker in salivary gland tumours (2011)
  • Author(s): Lateef E. Aljorani, Agnes Bankfalvi, Frank A. Carey, Koji Harada, Go Ohe, Sarah J. Jones, Ian R. Ellis, Seth L. Schor, Ana M. Schor
  • Journal Title: J Oral Pathol Med
  • Peer Reviewed
• [Journal Article] 口扁平上皮癌に対する経口抗癌剤TS-1を用いたメトロノミックケモテラピーの有用性とそのメカニズムの解析 (2011)
  • Author(s): 原田耕志
  • Journal Title: 学術の森 Volume: 4巻 Pages: 109-117
  • Peer Reviewed
• [Journal Article] 口腔扁平上皮癌における分子標的薬BortezomibとTS-1の併用効果についての基礎的研究 (2011)
  • Author(s): 原田豊子, 原田耕志, 上山吉哉
  • Journal Title: 頭頸部癌 Volume: 37 Pages: 536-541
  • NAID: 10030815616
  • Peer Reviewed
• [Journal Article] Effects of lentinan alone and in combination with fluoropyrimidine anticancer agent on growth of human oral squamous cell carcinoma in vitro and in vivo (2010)
  • Author(s): Koji Harada, Yasutaka Itashiki, Takanori Takenawa, Yoshiya Ueyama
  • Journal Title: Int J Oncol Volume: 37 Pages: 623-631
  • Peer Reviewed
• [Journal Article] Effects of lentinan alone and in combination with fluoropyrimidine anticancer agent on growth of human oral squamous cell carcinoma in vitro and in vivo. (2010)
  • Author(s): Koji Harada
  • Journal Title: Int J Oncol. Volume: 37 Pages: 623-631
  • Peer Reviewed
• [Journal Article] Effects of cepharanthine alone and in combination with fluoropyrimidine anticancer agent, S-1, on tumor growth of human oral squamous cell carcinoma xenografts in nude mice. (2010)
  • Author(s): Harada K
  • Journal Title: Anticancer Res. 29 Pages: 1263-1270
  • Peer Reviewed
• [Journal Article] Cepharanthine inhibits angiogenesis and tumorigenicity of human oral squamous cell carcinoma cells by suppressing expression of vascular endothelial growth factor and interleukin-8 (2009)
  • Author(s): Koji Harada, Tarannum Ferdous, Yasutaka Itashiki, Michiyo Takii, Takamitsu Mano, Yoshihide Mori, Yoshiya Ueyama
  • Journal Title: Int J Oncol Volume: 35 Pages: 1025-1035
  • Peer Reviewed
• [Journal Article] Effects of cepharanthine alone and in combination with fluoropyrimidine anticancer agent, S-1, on tumor growth of human oral squamous cell carcinoma xenografts in nude mice (2009)
  • Author(s): Koji Harada, Tarannum Ferdous, Yasutaka Itashiki, Michiyo Takii, Takamitsu Mano, Yoshihide Mori, Yoshiya Ueyama
  • Journal Title: Anticancer Res Volume: 29 Pages: 1263-70
  • Peer Reviewed
• [Journal Article] Clinical phase I trial of S-1 in thecombination with DOC using super-selective intra-arterial infusion with oral cancer (2009)
  • Author(s): Yoshiya Ueyama, Masaki Okafuji, Koji Harada, Takamitsu Mano, Mariko Mihara, Kenichiro Uchida, Daijyu Horinaga, Noriko Wada
  • Journal Title: Gan To Kagaku Ryoho Volume: 36 Pages: 395-399
  • Peer Reviewed
• [Journal Article] Cepharanthine inhibits angiogenesis and tumorigenicity of human oral squamous cell carcinoma cells by suppressing expression of vascular endothelial growth factor and interleukin-8. (2009)
  • Author(s): Harada K
  • Journal Title: Int J Oncol 35 Pages: 1025-1035
  • Peer Reviewed
• [Journal Article] Clinical phase I trial of S-1 in the combination with DOC using super-selective intra-arterial infusion with oral cancer. (2009)
  • Author(s): Ueyama Y
  • Journal Title: Gan To Kagaku Ryoho. 36 Pages: 395-399
  • Peer Reviewed
• [Presentation] 口腔癌に対するフッ化ピリミジン系経口抗癌剤を用いた新たな治療戦略 (2012)
  • Author(s): 原田耕志
  • Organizer: 日本口腔科学会総会
  • Place of Presentation: 広島国際会議場,広島
  • Year and Date: 2012-05-18
• [Presentation] Efficacy of metronomic chemotherapy with S-1 in human oral squamous cell carcinoma cells (2011)
  • Author(s): Koji Harada, Toyoko Harada, Yasutaka Itashiki, Takanori Takenawa, Yoshiya Ueyama
  • Organizer: The Third World Congress of the International Academy of Oral Oncology
  • Place of Presentation: the Copthorne Waterfront centre, Singapore, Singapore
  • Year and Date: 2011-07-17
• [Presentation] Efficacy of metronomic chemotherapy with S-1 in human oral squamous cell carcinoma cells (2011)
  • Author(s): Koji Harada, Toyoko Harada, Yasutaka Itashiki, Takanori Takenawa, Yoshiya Ueyama
  • Organizer: The Third World Congress of the International Academy of Oral Oncology
  • Place of Presentation: シンガポールシンガポール共和国
  • Year and Date: 2011-07-17
• [Presentation] Cepharanthine enhances radiosensitivity through the inhibition of DNA double-strand break repair in oral cancer cells (2010)
  • Author(s): Koji Harada, Kenichiro Uchida, Yoshiya Ueyama
  • Organizer: 日本癌学会
  • Place of Presentation: 国立京都国際会館,京都
  • Year and Date: 2010-09-23
• [Presentation] Cepharanthine enhances radiosensitivity through the inhibition of DNA double-strand break repair in oral cancer cells. (2010)
  • Author(s): Koji Harada
  • Organizer: 日本癌学会
  • Place of Presentation: 京都府 国立京都国際会館
  • Year and Date: 2010-09-23
• [Presentation] Effect of S-1 component Gimeracil on DNA double-strand break repair system (2009)
  • Author(s): Koji Harada, Yasutaka Itashiki, Michiyo Takii, Yuriko Suwa, Takamitsu Mano, Yoshihide Mori, Yoshiya Ueyama
  • Organizer: the Second World Congress of the International Academy of Oral Oncology
  • Place of Presentation: Sheraton Centre, Toronto, Canada
  • Year and Date: 2009-07-11
• [Presentation] Effect of S-1 component Gimeracil on DNA double-strand break repair system. (2009)
  • Author(s): Harada K
  • Organizer: the Second World Congress of the International Academy of Oral Oncology
  • Place of Presentation: Sheraton Centre, Toronto, Canada
  • Year and Date: 2009-07-11