Targeting oncogene AKT1 for novel oral cancer therapy

• Project/Area Number: 21592557
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Ehime University
• Principal Investigator: NAKASHIRO Koichi 愛媛大学, 大学院・医学系研究科, 准教授 (90314880)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 口腔癌 / AKT1 / RNA干渉 / siRNA / 分子標的治療

Research Abstract

Synthetic small interfering RNA targeting oncogene Akt1(siAkt1) was specifically delivered human oral squamous cell carcinoma(OSCC) xenografted tumors by atelocollagen-mediated systemic administration. Furthermore, we showed the usefulness of serum osteopontin and in vitro sensitivity test to predict the clinical effect of siAkt1. These results suggest that Akt1 functions as a critical oncogene in human OSCC cells and targeting Akt1 appears a novel approach for treatment with oral cancer.

Research Products

• [Journal Article] Premetastic vasculogenesis in oral squamous cell carcinoma xenograft-draining lymph nodes (2012)
  • Author(s): Mayorca-Guilliani AE, Yano H, Nakashiro K, Hamakawa H, Tanaka J
  • Journal Title: Oral Oncology Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Growth inhibition and apoptosis by an active component of OK-432, a streptococcal agent, via Toll-like receptor 4 in human head and neck cancer cell lines (2012)
  • Author(s): Tano T, Okamoto M, Kan S, Nakashiro K, Shimodaira S, Yamashita N, Kawakami Y, Hamakawa H
  • Journal Title: Oral Oncology Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Human FAT1 cadherin controls cell migration and invasion of oral squamous cell carcinoma through the localization ofβ-catenin (2011)
  • Author(s): Nishikawa Y, Miyazaki T, Nakashiro K, Yamagata H, Isokane M, Goda H, Tanaka H, Oka R, Hamakawa H.
  • Journal Title: Oncol Rep Volume: 26 Pages: 587-592
  • Peer Reviewed
• [Journal Article] Gene therapy for oral squamous cell carcinoma with IAI. 3B promoter-driven oncolytic adenovirus-infected carrier cells (2011)
  • Author(s): Zhang T, Hamada K, Hyodo M, Itoh H, Goda H, Nakashiro K, Hamakawa H.
  • Journal Title: Oncol Rep Volume: 25 Pages: 795-802
  • Peer Reviewed
• [Journal Article] Human FAT1 cadherin controls cell migration and invasion of oral squamous cell carcinoma through the localization of beta-catenin (2011)
  • Author(s): Nishikawa, Y.
  • Journal Title: Oncology Reports Volume: 26(3) Pages: 587-592
  • DOI: 10.3892/or.2011.1324
  • Peer Reviewed
• [Journal Article] Gene therapy for head and neck squamous carcinoma cells by IAI. 3B promoter-driven oncolytic adenovirus-infected carrier cells (2011)
  • Author(s): Zhang T, et al.
  • Journal Title: Oncol Rep Volume: 25 Pages: 795-802
  • Peer Reviewed
• [Journal Article] Friend leukaemia insertion(Fli)-1 is a prediction marker candidate for radiotherapy resistant oral squamous cell carcinoma (2010)
  • Author(s): Shintani S, Hamakawa H, Nakashiro K, Shirota T, Hatori M, Tanaka M, Kuroshita Y, Kurokawa Y.
  • Journal Title: Int J Oral Maxillofac Surg Volume: 39 Pages: 1115-1119
  • Peer Reviewed
• [Journal Article] Genome-wide cDNA microarray screening to reveal gene-expression profiles correlated to chemosensitivity of oral squamous cell carcinoma cell lines (2010)
  • Author(s): Shintani S, Nakashiro K, Hamakawa H, Shirota T, Hatori M., Kawamoto K, Sasaki A.
  • Journal Title: Asian J Oral Maxillofac Surg Volume: 22 Pages: 7-11
  • Peer Reviewed
• [Journal Article] Knockdown of Akt isoforms by RNA silencing suppresses the growth of human prostate cancer cells in vitro and in vivo (2010)
  • Author(s): Sasaki T, Nakashiro K, Tanaka H, Azuma K, Goda H, Hara S, Onodera J, Fujimoto I, Tanji N, Yokoyama M, Hamakawa H.
  • Journal Title: Biochem Biophys Res Commun Volume: 399 Pages: 79-83
  • Peer Reviewed
• [Journal Article] Spindle cell carcinoma after irradiated oral squamous cell carcinoma treated with S-1 (2010)
  • Author(s): Nakashiro K, Nishikawa H, Hamakawa H.
  • Journal Title: Asian J Oral Maxillofac Surg Volume: 22 Pages: 175-179
  • Peer Reviewed
• [Journal Article] Anti-tumor effect of small interfering RNA targeting the androgen receptor in human androgen-independent prostate cancer cells (2010)
  • Author(s): Azuma K, Nakashiro K, Sasaki T, Goda H, Onodera J, Tanji N, Yokoyama M, Hamakawa H.
  • Journal Title: Biochem Biophys Res Commun Volume: 391 Pages: 1075-1079
  • Peer Reviewed
• [Journal Article] Carrier cell-mediated cell lysis of squamous cell carcinoma cells by squamous cell carcinoma antigen 1 promoter-driven oncolytic adenovirus (2010)
  • Author(s): Hamada K, Zhang T, Desaki J., Nakashiro K, Itoh H, Tani K, Koyama Y, Hamakawa H.
  • Journal Title: J Gene Med Volume: 12 Pages: 545-554
  • Peer Reviewed
• [Journal Article] Conditional deletion of Stat3 promotes neurogenesis and inhibits astrogliogenesis in neural stem cells (2010)
  • Author(s): Cao F, Hata R, Zhu P, Nakashiro K, Sakanaka M.
  • Journal Title: Biochem Biophys Res Commun Volume: 394 Pages: 843-847
  • Peer Reviewed
• [Journal Article] Mandibular osteoblastic metastasis of poorly differentiated carcinoma of the thyroid gland (2010)
  • Author(s): Nishikawa T, Nakashiro K, Sumida T, Sugita A, Hamakawa H.
  • Journal Title: Int J Oral Maxillofac Surg Volume: 39 Pages: 301-304
  • Peer Reviewed
• [Journal Article] Anti-tumor effect of small interfering RNA targeting the androgen receptor in human androgen-independent prostate cancer cells (2010)
  • Author(s): Azuma K, Nakashiro K, et al.
  • Journal Title: Biochem Biophys Res Commun 391 Pages: 1075-1079
  • Peer Reviewed
• [Journal Article] Mandibular osteoblastic metastasis of poorly differentiated carcinoma of the thyroid gland (2010)
  • Author(s): Nishikawa H, Nakashiro K, et al.
  • Journal Title: Int J Oral Maxillofac Surg 39 Pages: 301-304
  • Peer Reviewed
• [Journal Article] 18F-fluorodeoxyglucose positron emission tomography computed tomography for the diagnosis of cervical lymph-node metastases of oral squamous cell carcinoma (2009)
  • Author(s): Nishikawa T, Nakashiro K, Sumida T, Miyagawa M, Hamakawa H.
  • Journal Title: Asian J Oral Maxillofac Surg Volume: 21 Pages: 88-95
  • Peer Reviewed
• [Journal Article] Hypoxia enhances CXCR4 expression by activating HIF-1 in oral squamous cell carcinoma (2009)
  • Author(s): Ishikawa T, Nakashiro K, Klosek SK, Goda H, Hara S, Uchida D, Hamakawa H.
  • Journal Title: Oncol Rep Volume: 21 Pages: 707-712
  • Peer Reviewed
• [Journal Article] CD151 regulates HGF-stimulated morphogenesis of human breast cancer cells (2009)
  • Author(s): Klosek SK, Nakashiro K, Hara S, Goda H, Hasegawa H, Hamakawa H.
  • Journal Title: Biochem Biophys Res Commun Volume: 379 Pages: 1097-1100
  • Peer Reviewed
• [Journal Article] 18F-Fluorodeoxyglucose positron emission tomography-computed tomography for diagnosis of cervical lymph node metastasis of oral squamous cell carcinoma (2009)
  • Author(s): Nishikawa H, Nakashiro K, et al.
  • Journal Title: Asian J Oral Maxillofac Surg 21 Pages: 88-95
  • Peer Reviewed
• [Presentation] ヒト口腔扁平上皮癌細胞の癌遺伝子依存 (2010)
  • Author(s): 中城公一
  • Organizer: 第55回日本口腔外科学会総会・学術大会
  • Place of Presentation: 幕張
  • Year and Date: 2010-10-27
• [Presentation] ヒト口腔扁平上皮癌細胞の癌遺伝子依存 (2010)
  • Author(s): 中城公一
  • Organizer: 第55回日本口腔外科学会総会・学術大会
  • Place of Presentation: 幕張メッセ(千葉県)
  • Year and Date: 2010-10-27
• [Presentation] 口腔扁平上皮癌の原発およびリンパ節転移腫瘍における癌関連遺伝子発現プロファイル (2009)
  • Author(s): 中城公一, 合田啓之, 住田知樹, 浜川裕之
  • Organizer: 第54回日本口腔外科学会総会・学術大会
  • Place of Presentation: 札幌
• [Presentation] 口腔扁平上皮癌における癌関連遺伝子発現の経時的変化 (2009)
  • Author(s): 中城公一, 合田啓之, 住田知樹, 浜川裕之
  • Organizer: 第33回日本頭頸部癌学会総会
  • Place of Presentation: 札幌
• [Patent(Industrial Property Rights)] マイクロRNA又はその発現系を含む組成物 (2011)
  • Inventor(s): 中城公一、浜川裕之、田中宏史
  • Industrial Property Rights Holder: 愛媛大学
  • Industrial Property Number: 2011-113348
  • Filing Date: 2011-05-20
• [Patent(Industrial Property Rights)] マイクロRNAのアンチセンスオリゴヌクレオチドを含む組成物 (2010)
  • Inventor(s): 中城公一、浜川裕之、田中宏史
  • Industrial Property Rights Holder: 愛媛大学
  • Industrial Property Number: 2010-254021
  • Filing Date: 2010-11-12
• [Patent(Industrial Property Rights)] マイクロRNAのアンチセンスオリゴヌクレオチドを含む組成物 (2010)
  • Inventor(s): 中城公一, 浜川裕之, 田中宏史
  • Industrial Property Rights Holder: 愛媛大学
  • Industrial Property Number: 2010-254021
  • Filing Date: 2010-11-12
• [Patent(Industrial Property Rights)] 頸部リンパ節転移分析方法及び頭頸部癌の腫瘍マーカー (2009)
  • Inventor(s): 中城公一、浜川裕之、合田啓之
  • Industrial Property Rights Holder: 愛媛大学
  • Industrial Property Number: 2009-292611
  • Filing Date: 2009-12-24
• [Patent(Industrial Property Rights)] 頸部リンパ節転移分析方法及び頭頸部癌の腫瘍マーカー (2009)
  • Inventor(s): 中城公一, 浜川裕之, 合田啓之
  • Industrial Property Rights Holder: 愛媛大学
  • Industrial Property Number: 2009-292611
  • Filing Date: 2009-12-24