| Project/Area Number |
21592564
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
TAKADA Eiko 東京医科大学, 医学部, 講師 (50110903)
HATA Kikumi 東京医科大学, 医学部, 助教 (30287156)
TOYOTA Hiroko 東京医科大学, 医学部, 助手 (80468660)
CHIBA Hiroshige 東京医科大学, 医学部, 教授 (80105478)
MATSUO Akira 東京医科大学, 医学部, 講師 (70229417)
SATOMI Takashi 東京医科大学, 医学部, 講師 (70276921)
WATANABE Masato 東京医科大学, 医学部, 講師 (40349460)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 口腔扁平上皮癌 / 癌幹細胞 / 治療戦略 |
|---|
| Research Abstract |
Several colonies were found when oral squamous cell carcinoma cells were cultured with DMEM/F12 medium in the presence of epidermal growth factor and fibroblast growth factor for 2 or 3 weeks. Cells prepared from the colony were CD24-negative and CD44-positive(CD24-/CD44+), as assessed by flow cytometry using monoclonal antibodies. The CD24-/CD44+cells constituted only a minor population(around 6%) of original cell line and showed resistance to chemotherapeutic agent(carboplatin) and increased capacity to form colony, compared with controls. Thus, this CD24-/CD44+minor population behaves like cancer stem cells for SCC. The increased proportion of CD24-/CD44+cells and capacity of colony formation were detected in SCC25 cells over-expressing constitutively active form of Akt, compared with control vector alone, suggesting that Akt activation pathway participates in the acquisition and/or maintenance of cancer stem cells for oral carcinoma cells. Thus, Akt signaling pathway would be a new target for therapeutic intervention of patients with oral squamous cell carcinoma.
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