Bone regeneration with osteoblast gel and artificial bone marrow which can produce interaction between stem cells

• Project/Area Number: 21592573
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Okayama University
• Principal Investigator: YAMACHIKA Eiki 岡山大学, 岡山大学病院, 講師 (10294422)
• Co-Investigator(Kenkyū-buntansha): TSUJIGIWA Hidetsugu 岡山大学, 大学院・医歯薬学総合研究科, 准教授 (70335628)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 骨再生 / 成体幹細胞 / 間葉系幹細胞 / マウス

Research Abstract

We developed methods to isolate, purify, and expand mesenchymal stem cells(MSCs) from mouse compact bone that may be used to regenerate bone in vivo. These cells were maintained in long-termculture and were capable of differentiating along multiple lineages, including chondrocyte, osteocyte, and adipocyte trajectories. We found that compact bone of mice was a better source of MCSs than the bone marrow, that growth in plastic flasks served to purify MSCs from hematopoietic cells, and that MSCs grown in basic fibroblast growth factor(bFGF)-conditioned medium were, based on multiple criteria, superior to those grown in leukemia inhibitory factor-conditioned medium. Moreover, we found that the MSCs isolated from compact bone and grown in bFGF-conditioned medium were capable of supporting bone formation in vivo.

Research Products

• [Journal Article] Iida S Basic fibroblast growth factor supports expansion of mouse compact bone-derived mesenchymal stem cells(MSCs) and regeneration of bone from MSC in vivo (2012)
  • Author(s): Yamachika E, Tsujigiwa H, Matsubara M, Hirata Y, Kita K, Takabatake K, Mizukawa N, Kaneda Y, Nagatsuka H
  • Journal Title: J Mol Histol Volume: 43(2) Pages: 223-33
  • Peer Reviewed
• [Journal Article] Differential expression of canonical and non-canonical Wnt ligandsin ameloblastoma (2012)
  • Author(s): Siar CH, Nagatsuka H, Han PP, Buery RR, Tsujigiwa H, Nakano K, Ng KH, Kawakami T
  • Journal Title: J Oral Pathol Med Volume: 41(4) Pages: 332-9
  • Peer Reviewed
• [Journal Article] Notch signaling and ghost cell fate in the calcifying cystic odontogenic tumor (2011)
  • Author(s): Siar CH, Kawakami T, Buery RR, Nakano K, Tomida M, Tsujigiwa H, Han PP, Nagatsuka H, Ng KH
  • Journal Title: Eur J Med Res Volume: 16(11) Pages: 501-6
  • Peer Reviewed
• [Journal Article] Dickkopf(Dkk)-3 andβ-catenin expressions increased in the transition from normal oral mucosal to oral squamous cell carcinoma (2011)
  • Author(s): Fujii M, Katase N, Lefeuvre M, Gunduz M, Buery RR, Tamamura R, Tsujigiwa H, Nagatsuka H
  • Journal Title: J Mol Histol Volume: 42(6) Pages: 499-504
  • Peer Reviewed
• [Journal Article] Effect of fluoride-substituted apatite on in vivo bone formation (2011)
  • Author(s): Inoue M, Rodriguez AP, Nagai N, Nagatsuka H, LeGeros RZ, Tsujigiwa H, Inoue M, Kishimoto E, Takagi S
  • Journal Title: J Biomater Appl Volume: 25(8) Pages: 811-24
  • Peer Reviewed
• [Journal Article] Basic fibroblast growth factor supports expansion of mouse compact bone-derived mesnchymal stem cells (MSCs) and regeneration of bone fome MSC (2011)
  • Author(s): Eiki Yamachika
  • Journal Title: Jorunal of Molecular Histology
  • Peer Reviewed
• [Journal Article] Immunolocalization of notch signaling protein molecules in a maxillary chondrosarcoma and its recurrent tumor (2010)
  • Author(s): Siar CH, Ha KO, Aung LO, Nakano K, Tsujigiwa H, Nagatsuka H, Ng KH, Kawakami T
  • Journal Title: Eur J Med Res Volume: 15(10) Pages: 456-60
  • Peer Reviewed
• [Journal Article] Allelic loss of the ING gene family loci is a frequent event inameloblastoma (2010)
  • Author(s): Borkosky SS, Gunduz M, Beder L, Tsujigiwa H, Tamamura R, Gunduz E, Katase N, Rodriguez AP, Sasaki A, Nagai N, Nagatsuka H
  • Journal Title: Oncol Res Volume: 18(10) Pages: 509-18
  • Peer Reviewed
• [Journal Article] Preferential up-regulation of heparanase and cyclooxygenase-2 in carcinogenesis of Barrett's oesophagus and intestinal-typegastric carcinoma (2010)
  • Author(s): Sonoda R, Naomoto Y, Shirakawa Y, Fujiwara Y, Yamatsuji T, Noma K, Tanabe S, Takaoka M, Gunduz M, Tsujigiwa H, Nagatsuka H, Ohara N, Yoshino T, Takubo K, Vieth M, Tanaka N
  • Journal Title: Histopathology Volume: 57(1) Pages: 90-100
  • Peer Reviewed
• [Journal Article] The early administration of granulocyte colony-stimulating factor increases the engraftment of transplanted bone marrow-derived cells into the olfactory epithelium damaged by methimazole (2010)
  • Author(s): Nishizaki K, Yoshinobu J, Tsujigiwa H, Orita Y, Yamada M
  • Journal Title: Rhinology Volume: 48(2) Pages: 228-32
  • Peer Reviewed
• [Journal Article] Suppressive effects of 1, 4-dihydroxy-2-naphthoic acid administration on bone resorption (2010)
  • Author(s): Matsubara M, Yamachika E, Tsujigiwa H, Mizukawa N, Ueno T, Murakami J, Ishida N, Kaneda Y, Shirasu N, Takagi S
  • Journal Title: Osteoporos Int Volume: 21(8) Pages: 1437-47
  • Peer Reviewed
• [Journal Article] Suppressive effects of 1,4-dihydroxy-2-naphthoic acid administration on bone resorption. (2010)
  • Author(s): Matsubara M
  • Journal Title: Osteoporos Int. Volume: 21 Pages: 1437-1447
  • Peer Reviewed
• [Journal Article] Bone formation in a rat calvarial defect model after transplanting autogenous bone marrow with beta-tricalcium phosphate. (2010)
  • Author(s): Shirasu N
  • Journal Title: Acta Histochem. Volume: 112 Pages: 270-277
  • Peer Reviewed
• [Journal Article] Immobilized recombinant human bone morphogenetic protein-2 enhances the phosphorylation of receptor-activated Smads. (2009)
  • Author(s): Yamachika E
  • Journal Title: J Biomed Mater Res A. 88 Pages: 599-607
  • Peer Reviewed
• [Journal Article] Suppressive effects of 1, 4-dihydroxy-2-naphthoic acid administration on bone resorption. (2009)
  • Author(s): Matsubara M
  • Journal Title: Osteoporos Int. (in press)
  • Peer Reviewed
• [Journal Article] Localization of antimicrobial peptides human beta-defensins in minor salivary glands with Sjogren's syndrome. (2009)
  • Author(s): Kaneda Y,
  • Journal Title: Eur J Oral Sci. 117 Pages: 506-510
  • Peer Reviewed
• [Presentation] 1, 4-dihydroxy-2-naphthoic acid(DHNA)を用いた骨粗鬆症に対する新規治療法の検討 (2011)
  • Author(s): 松原正和、山近英樹、喜多憲一郎、高畠清文、藤田佑貴、高木愼、石田展久、花野響子、飯田征二
  • Organizer: 第56回日本口腔外科学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2011-10-21
• [Presentation] Anti Osteoporotic Effects of 1, 4-dihydroxy-2-naphthoic acid (2011)
  • Author(s): Eiki Yamachika, Matsubara Masakazu, Ken-ichiro Kita, Kiyofumi Takabatake, Yuki Fujita, Nobuyoshi Mizukawa, Tatsushi Matsumura, Hidetsugu Tsujigiwa, Hitoshi Nagatsuka, Seiji Iida
  • Organizer: American Society for Bone and Mineral Research 2011 annual meeting
  • Place of Presentation: San Diego, Ca, USA
  • Year and Date: 2011-09-17
• [Presentation] Anti Osteoporotio Effects of 1,4-dihydroxy-2-naphthoic acid (2011)
  • Author(s): Eiki Yamachika
  • Organizer: American society for bone and mineral research annual meeting 2011
  • Place of Presentation: San Diego
  • Year and Date: 2011-09-17
• [Presentation] An alternative treatment for osteoporosis with DHNA-microCT analysis (2011)
  • Author(s): Kita K, Yamachika E, Matsubara M, Fujita Y, Iida S, Asaumi J, Murakami J, Arai Y, Honda K, Ejima K
  • Organizer: 18th International Congress of Dento-Maxillo-Facial Radiology
  • Place of Presentation: Hiroshima
  • Year and Date: 2011-05-29
• [Presentation] An alternative treatment for osteoporosis with DHNA (2011)
  • Author(s): Kita K
  • Organizer: 18th International Congress of Dento-Maxillo-Facial Radiology
  • Place of Presentation: 広島
  • Year and Date: 2011-05-26
• [Presentation] OVXマウスによる骨粗鬆症への1, 4-dihydroxy-2-naphthoic acid(DHNA)を用いた治療効果の検討 (2011)
  • Author(s): 高畠清文、松原正和、山近英樹、喜多憲一郎、藤田佑貴、浅海淳一、辻極秀次、長塚仁、飯田征二
  • Organizer: 第65回日本口腔科学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2011-04-21
• [Presentation] マウス骨由来間葉系幹細胞による骨再生の基礎的研究 (2010)
  • Author(s): 山近英樹、松原正和、喜多憲一郎、武田斉子、池谷陽子、水川展吉、飯田征二
  • Organizer: 第55回日本口腔外科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2010-10-17
• [Presentation] OVXマウスにおける1, 4-dihydroxy-2-naphthoic acid(DHNA)の骨吸収抑制効果について (2010)
  • Author(s): 喜多憲一郎、松原正和、山近英樹、香川智正、高木愼、石田展久、平田泰久、藤田佑貴、高畠清文、飯田征二
  • Organizer: 第55回日本口腔外科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2010-10-17
• [Presentation] 1, 4-dihydroxy-2-naphthoic acid(DHNA)の骨吸収抑制メカニズムについての検討 (2010)
  • Author(s): 松原正和、喜多憲一郎、山近英樹、香川智正、平田泰久、藤田佑貴、飯田征二
  • Organizer: 第55回日本口腔外科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2010-10-17
• [Presentation] マウス骨髄由来間葉系細胞の培養におけるLifおよびb-FGFの効果 (2010)
  • Author(s): 山近英樹、松原正和、喜多憲一郎、武田斉子、池谷陽子、藤田佑貴、松村達志、水川展吉、辻極秀次、長塚仁、飯田征二
  • Organizer: 第64回日本口腔科学会学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2010-06-25
• [Presentation] 1, 4-dihydroxy-2-naphthoic acid(DHNA)による骨吸収抑制効果についての検討 (2010)
  • Author(s): 松原正和、山近英樹、喜多憲一郎、藤田佑貴、平田泰久、松村達志、辻極秀次、長塚仁、飯田征二
  • Organizer: 第64回日本口腔科学会学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2010-06-25
• [Presentation] マウス骨髄由来間葉系細胞の培養におけるLifおよびb-FGFの効果 (2010)
  • Author(s): 山近英樹
  • Organizer: 第64回NPO法人 日本口腔科学会
  • Place of Presentation: 札幌
• [Presentation] マウス骨からの間葉系幹細胞の単離 (2009)
  • Author(s): 山近英樹, 松原正和, 喜多憲一郎, 金田祥弘, 武田斉子, 水川展吉
  • Organizer: 第54回日本口腔外科学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2009-10-09
• [Presentation] 培養骨髄細胞の3次元的構造体による骨形成能の検討 (2009)
  • Author(s): 喜多憲一郎, 山近英樹, 松原正和, 高木慎, 石田展久, 平田泰久
  • Organizer: 第63回日本口腔科学会総会
  • Place of Presentation: 浜松
  • Year and Date: 2009-04-16