The research for the niche factor of stem cells in culture condition
| Project/Area Number |
21592584
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
KIKUIRI Takashi 北海道大学, 大学院・歯学研究科, 助教 (10322819)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIMURA Yoshitaka 北海道大学, 大学院・歯学研究科, 助教 (30230816)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 乳歯歯髄由来幹細胞 / 未分化維持 / 継代培養 / Wntシグナル / 歯学 / 歯根膜由来幹細胞 / 分化 |
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| Research Abstract |
Stem cells from human exfoliated deciduous teeth(SHED) are stem cell that differentiate into osteocyte and adipocyte. In this study, we investigated whether the Wnt signaling inhibitor has a potency of the stemness of SHED after long-term cell culture or not. We demonstrated that SHED treated with the Wnt signaling inhibitor was capable of stemness in vitro experiment and generating bone structures at 8 weeks post-transplantation into nude mouse. These data suggested that the Wnt signaling inhibitor acts as a stemness factor on SHED after long-term cell culture
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Report
(4 results)
Research Products
(9 results)
