The research for the niche factor of stem cells in culture condition

• Project/Area Number: 21592584
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Hokkaido University
• Principal Investigator: KIKUIRI Takashi 北海道大学, 大学院・歯学研究科, 助教 (10322819)
• Co-Investigator(Kenkyū-buntansha): YOSHIMURA Yoshitaka 北海道大学, 大学院・歯学研究科, 助教 (30230816)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 乳歯歯髄由来幹細胞 / 未分化維持 / 継代培養 / Wntシグナル / 歯学 / 歯根膜由来幹細胞 / 分化

Research Abstract

Stem cells from human exfoliated deciduous teeth(SHED) are stem cell that differentiate into osteocyte and adipocyte. In this study, we investigated whether the Wnt signaling inhibitor has a potency of the stemness of SHED after long-term cell culture or not. We demonstrated that SHED treated with the Wnt signaling inhibitor was capable of stemness in vitro experiment and generating bone structures at 8 weeks post-transplantation into nude mouse. These data suggested that the Wnt signaling inhibitor acts as a stemness factor on SHED after long-term cell culture

Research Products

• [Journal Article] Stage-dependent suppression of the formation of dentin-resorbingmultinuclear cells with MIF in vitro (2011)
  • Author(s): Kikuiri Takashi, Yoshimura Yoshitaka, Tabata Futoshi, Hasegawa Tomokazu, Nishihira Jun, Shirakawa Testuo
  • Journal Title: Experimental and Therapeutic Medicine Volume: 3 Pages: 37-43
  • Peer Reviewed
• [Journal Article] Platinum nanoparticles suppress osteoclastogenesis through scavenging of ROS productions in RAW 264. 7 cells (2011)
  • Author(s): Mayumi Nomura, Yoshitaka Yoshimura, Takashi Kikuiri, Tomokazu Hasegawa, Yumi Taniguchi, Yoshiaki Deyama, Ken-ichi Koshiro, Hidehiko Sano, Kuniaki Suzuki and Nobuo Inoue
  • Journal Title: Journal of Pharmacological Sciences Volume: 117 Pages: 243-252
  • Peer Reviewed
• [Journal Article] Effect of the release from mechanical stress on osteoclastogenesis in RAW 264. 7 cells (2011)
  • Author(s): Kenjiro Shibata, Yoshitaka Yoshimura, Takashi Kikuiri, Tomokazu Hasegawa, Yumi Taniguchi, Yoshiaki Deyama, Kuniaki Suzuki and Junichiro Iida
  • Journal Title: International Journal of Molecular Medicine Volume: 28(1) Pages: 73-79
  • Peer Reviewed
• [Journal Article] Stage-dependent suppression of the formation of dentin-resorbing multinuclear cells with MIF in vitro (2011)
  • Author(s): Kikuiri T, Yoshimura Y, Tabata F, Hasegawa T, Nishihira J, Shirakawa T
  • Journal Title: Experimental and Therapeutic Medicine Volume: 3 Issue: 1 Pages: 37-43
  • DOI: 10.3892/etm.2011.362
  • Peer Reviewed
• [Journal Article] Effect of the release from mechanical stress on osteoclastogenesis in RAW 264. 7 cells (2011)
  • Author(s): Shibata K, Yoshimura Y, Kikuiri T, Hasegawa T, Taniguchi Y, Deyama Y, Suzuki K, Iida J.
  • Journal Title: Int J Molecular Medicin Volume: 28 Pages: 73-79
  • DOI: 10.3892/ijmm.2011.675
  • Peer Reviewed
• [Journal Article] Platinum nanoparticles suppress osteoclastogenesis through scavenging of reactive oxygen species produced in RAW264.7 cells (2011)
  • Author(s): Nomura M, Yoshimura Y, Kikuiri T, Hasegawa T, Taniguchi Y, Deyama Y, Koshiro K, Sano H, Suzuki K, Inoue N
  • Journal Title: J Pharmacol Sci Volume: 117 Pages: 243-252
  • NAID: 10030453660
  • Peer Reviewed
• [Journal Article] Effect of the release from mechanical stress on osteoclastogenesis in RAW264.7 cells (2011)
  • Author(s): KENJIRO SHIBATA
  • Journal Title: International Journal of Molecular Medicine Volume: (印刷中,印刷中)
  • Peer Reviewed
• [Presentation] 間葉系幹細胞と造血系幹細胞の併用による骨組織再生の試み (2011)
  • Author(s): 松野美乃、菊入崇、佐藤嘉晃、飯田順一郎、鈴木邦明
  • Organizer: 第70回日本矯正歯科学会大会&第4回国際会議
  • Place of Presentation: 名古屋国際会議場(名古屋市)
  • Year and Date: 2011-10-17
• [Presentation] 間葉系幹細胞と造血系幹細胞の併用による骨組織再生の試み (2011)
  • Author(s): 松野美乃、菊入崇、佐藤嘉晃、飯田順一郎、鈴木邦明
  • Organizer: 第70回日本矯正歯科学会大会 & 第4回国際会議
  • Place of Presentation: 名古屋市・名古屋国際会議場