An analysis of the role of FGF receptors in early mandibular patterning by using RNA interference

• Project/Area Number: 21592587
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Kyushu University (2010-2011); Tohoku University (2009)
• Principal Investigator: TERAO Fumie 九州大学, 大学院・歯学研究院, 助教 (10510018)
• Co-Investigator(Kenkyū-buntansha): TAKAHASHI Ichiro 九州大学, 大学院・歯学研究院, 教授 (70241643)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: FGF / 受容体 / 下顎隆起 / 器官形成 / RNA / 干渉 / FGF受容体 / RNA干渉 / 器官培養

Research Abstract

We investigated the gene expression pattern of FGFR genes in early mandibular development in rats. In the mandibular cell micromass culture, the activation of FGF-FGFR signal by recombinant FGF treatment enhanced endogenous ERK(extracellular signal-regulated kinase) phosphorylation. The transfection of induced by the electroporation of an Fgfr2c short hairpin RNA(shRNA) vector altered the shape of Meckel' s cartilage. These results indicate that FGFR plays an important role during early mandibular morphogenesis.

Research Products

• [Journal Article] Effects of retraction of anterior teeth and initial softtissue variables on lip changes in Japanese adults (2012)
  • Author(s): Hayashida H., et al
  • Journal Title: Eur J Orthod Volume: 33(4) Issue: 4 Pages: 419-426
  • DOI: 10.1093/ejo/cjq095
  • Peer Reviewed
• [Journal Article] Three-dimensional analysis of the pharyngeal airway morphology in growing Japanese females with and without cleft lip and palate (2012)
  • Author(s): Yoshihara M., et al
  • Journal Title: Am J Orthod Dentofacial Orthopedics Volume: (in press)
  • Peer Reviewed
• [Journal Article] Fibroblast growth factor 10 regulates Meckel' s cartilage formation d uring early mandibular morphogenesis in rats (2011)
  • Author(s): Terao F, Takahashi I, Mitani H, Haruya ma N, Sasano Y, Suzuki O, Takano-Ya mamoto T.
  • Journal Title: Dev. Biol Volume: 350 Pages: 337-347
  • Peer Reviewed
• [Journal Article] Comparison of orthodontic treatment outcomes in adults with skeletal open bite between conventional edgewise treatment and implant-anchored orthodontics (2011)
  • Author(s): Deguchi T., et al
  • Journal Title: Am J Orthod Dentofacial Orthopedics Volume: 139(4) Issue: 4 Pages: 60-68
  • DOI: 10.1016/j.ajodo.2009.04.029
  • Peer Reviewed
• [Journal Article] The primary site of the acrocephalic feature in Apert syndrome is a dwarf cranical base with accelerated chondrocytic differentiation due to aberrant activation of the FGFR2 signaling (2011)
  • Author(s): Nagata M., et al
  • Journal Title: Bone Volume: 48 Issue: 4 Pages: 847-856
  • DOI: 10.1016/j.bone.2010.11.014
  • Peer Reviewed
• [Journal Article] Three-dimensional analysis of the temporomandibular joint and fossa-condyle relationship (2011)
  • Author(s): Endo M., et al
  • Journal Title: Orthodontics Volume: 12(3) Pages: 210-221
  • Peer Reviewed
• [Journal Article] Involvement of PRIP, Phospholipase C-related, but Catalytically Inactive Protein, in Bone Formation (2011)
  • Author(s): Koshiro Tsutsumi
  • Journal Title: Journal of Biological Chemistry Volume: 286 Issue: 35 Pages: 31032-31042
  • DOI: 10.1074/jbc.m111.235903
  • Peer Reviewed
• [Journal Article] Fibroblast growth factor 10 regulates Meckel's cartilage formation during early mandibular morphogenesis in rats. (2011)
  • Author(s): Terao F, Takahashi I, Mitani H, Haruyama N, Sasano Y, Suzuki O, Takano-Yamamoto T.
  • Journal Title: Dev.Biol. Volume: 350 Pages: 337-347
  • Peer Reviewed
• [Journal Article] Mandibular body lengthening by distraction osteogenesis for correction of skeletal class II problems with an impacted premolar (2010)
  • Author(s): Takahashi I, Terao F, Suzuki M, Kawamura H, Takano-Yamamoto T.
  • Journal Title: J. Oral. Maxillofac. Surg Volume: 68 Pages: 2893-2902
  • Peer Reviewed
• [Journal Article] Takano-Yamamoto T Combined maxillary and mandibular midline and mandibular ramous distraction osteogenesis for treatment of a Class II patient with use of implants as orthodontic anchorages (2010)
  • Author(s): Takahashi I, Kawamura H
  • Journal Title: Am J Orthod Dentofac Orthop Volume: 137 Pages: 412-423
  • Peer Reviewed
• [Journal Article] Itoi E Expression of collagen type I and II on articular cartilage in a rat knee contracture model (2010)
  • Author(s): Hagiwara Y, Ando A, Chimoto E, Tsuchiya M, Takahashi I, Sasano Y, Onoda Y, Suda H
  • Journal Title: Connect Tissue Res Volume: 51 Pages: 22-30
  • Peer Reviewed
• [Journal Article] Kikuchi M Induction of MMP-2 at the interface between epithelial cells and fibroblasts from human periodontal ligament (2010)
  • Author(s): Shimonishi M, Takahashi I, Terao F, Komatsu M
  • Journal Title: J Periodontal Res Volume: 45 Pages: 309-316
  • Peer Reviewed
• [Journal Article] Mandibular body lengthening by distraction osteogenesis for correction of skeletal class II problems with an impacted premolar (2010)
  • Author(s): Takahashi I, Terao F, Suzuki M, Kawamura H, Takano-Yamamoto T.
  • Journal Title: J.Oral.Maxillofac.Surg. Volume: 68 Pages: 2893-2902
  • Peer Reviewed
• [Journal Article] Combined maxillary and mandibular midline and mandibular ramous distraction osteogenesis for treatment of a Class II patient with use of implants as orthodontic anchorages. (2010)
  • Author(s): Takahashi I, Kawamura H, Takano-Yamamoto T
  • Journal Title: Am J Orthod Dentofac Orthop. 137 Pages: 412-423
  • Peer Reviewed
• [Journal Article] Expression of collagen type I and II on articular cartilage in a rat knee contracture model. (2010)
  • Author(s): Hagiwara Y, Ando A, Chimoto E, Tsuchiya M, Takahashi I, Sasano Y, Onoda Y, Suda H, Itoi E
  • Journal Title: Connect Tissue Res. 51 Pages: 22-30
  • Peer Reviewed
• [Journal Article] Induction of MMP-2 at the interface between epithelial cells and fibroblasts from human periodontal ligament. (2010)
  • Author(s): Shimonishi M, Takahashi I, 寺尾文恵, Komatsu M, Kikuchi M
  • Journal Title: J Periodontal Res. 45 Pages: 309-316
  • Peer Reviewed
• [Journal Article] Suzuki O Molecular Mechanisms of Mechanical Stress Response during Chondrogenesis J Biochem Sci Eng. (2009)
  • Author(s): Takahashi I, Masuda T, Kohsaka K, Terao F, Anada T, Sasano Y, Takano-YamamotoTakahashi I, Masuda T, Kohsaka K, Terao F, Anada T, Sasano Y, Takano-YamamotoTakahashi I, Masuda T, Kohsaka K, Terao F, Anada T, Sasano Y, Takano-Yamamoto T.
  • Volume: 4
  • Pages: 307-317
  • Peer Reviewed
• [Journal Article] Watanabe M Synthesi s and intercellular transportation of type I procollagen during functional differen tiation of odontoblast (2009)
  • Author(s): Sato S, Tsuchiya M, Komaki K, Kusun oki S, Tsuchiya S, Haruyama N, Takah ashi I, Sasano Y
  • Journal Title: Histochem Cell B iol. Histochem Cell B iol Volume: 131 Pages: 583-591
  • Peer Reviewed
• [Journal Article] 下顎器官形成期におけるFGF10のメッケル軟骨の形態形成に果たす役割 (2009)
  • Author(s): 寺尾文恵
  • Journal Title: 東北大学歯学会雑誌 28
  • Peer Reviewed
• [Journal Article] Molecular Mechanisms of Mechanical Stress Response during Chondrogenesis (2009)
  • Author(s): Takahashi I, Masuda T, Kohsaka K, 寺尾文恵, Anada T, Sasano Y, Takano-Yamamoto T, Suzuki O
  • Journal Title: J Biochem Sci Eng 4 Pages: 307-317
  • NAID: 130000129002
  • Peer Reviewed
• [Journal Article] Comparison of expression patterns of cathepsin K and MMP-9 in odontoclasts and osteoclasts in physiological root resorption in the rat molar. (2009)
  • Author(s): Tsuchiya M, Akiba Y, Takahashi I, Sasano Y, Kashiwazaki J, Tsuchiya S, Watanabe M
  • Journal Title: Arch Histol Cytol. 71 Pages: 89-100
  • NAID: 10025095642
  • Peer Reviewed
• [Journal Article] Synthesis and intercellular transportation of type I procollagen during functional differentiation of odontoblast. (2009)
  • Author(s): Sato S, Tsuchiya M, Komaki K, Kusunoki S, Tsuchiya S, Haruyama N, Takahashi I, Sasano Y, Watanabe M
  • Journal Title: Histochem Cell Biol. 131 Pages: 583-591
  • Peer Reviewed
• [Presentation] 胎生期下顎形成期におけるFGF10によるメッケル軟骨形態の制御 (2011)
  • Author(s): 寺尾文恵
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪)
  • Year and Date: 2011-07-28
• [Presentation] FGF10 contributes Meckel' s cartilage formation in early mandibular development (2011)
  • Author(s): Terao F
  • Organizer: The 6th International Workshop on Nano-, Bio-and Amorphous Materials
  • Place of Presentation: Zao, Miyagi
• [Presentation] 胎生期下顎形成期におけるFGF10によるメッケル軟骨形態の制御 (2011)
  • Author(s): 寺尾文恵、高橋一郎、三谷英稔、春山直人、笹野泰之、鈴木治、山本照子
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
• [Presentation] FGF10 contributes Meckel's cartilage formation in early mandibular development (2011)
  • Author(s): Terao F
  • Organizer: The 6th International Workshop on Nano-, Bio- and Amorphous Materials
  • Place of Presentation: Zao, Miyagi
• [Presentation] ミニスクリューを固定源として用いたラビアルおよびリンガルブラケット装置による臨床評価の比較・検討 (2010)
  • Author(s): 出口徹、寺尾文恵、菅原康代、片岡伴記、山城隆、山本照子
  • Organizer: ミニスクリューを固定源として用いたラビアルおよびリンガルブラケット装置による臨床評価の比較・検討
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2010-09-29
• [Presentation] 機械的伸展刺激はERK-1/2の活性化を介して軟骨細胞分化を阻害する (2010)
  • Author(s): 高坂久美子、高橋一郎、益田泰輔、寺尾文恵、鈴木治、山本照子
  • Organizer: 第69回日本矯正歯科学会大会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2010-09-29
• [Presentation] ニスクリューを固定源として用いたラビアルおよびリンガルブラケット装置による臨床評価の比較・検討 (2010)
  • Author(s): 出口徹、寺尾文恵、菅原康代、片岡伴記、山城隆、山本照子
  • Organizer: 第69回日本矯正歯科学会大会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2010-09-29
• [Presentation] Fibroblast growth factor 10 and Meckel' s cartilage formation in the early mandibular morphogenesis (2010)
  • Author(s): Terao F, Takahashi I, Mitani H, Haruyama N, Sasano Y, Suzuki O, Takano-Yamamoto T
  • Organizer: 第5回口腔組織の再生・再建医療研究国際シンポジウム
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 2010-02-05
• [Presentation] Fibroblast growth factor 10 and Meckel's cartilage formation in the early mandibular morphogenesis. (2010)
  • Author(s): Terao F, Takahashi I, Mitani H, Haruyama N, Sasano Y, Suzuki O, Takano-Yamamoto T
  • Organizer: 第5回口腔組織の再生・再建医療研究国際シンポジウム
  • Place of Presentation: 福岡県・福岡市
  • Year and Date: 2010-02-05
• [Presentation] Suzuki O Intracellular signal transduction during mechano-response i n differentiating chondrocytes (2009)
  • Author(s): Takahashi I, Masuda T, Kohsaka K, Te rao F, Anada T, Sasano Y, Takano-Ya mamoto T
  • Organizer: JSPS-KO SEF Asian Core Program, Nano/Amorph ous Materials and Interface Science Sy mposiu
  • Place of Presentation: Tohgatta, Miyagi
  • Year and Date: 2009-08-07
• [Presentation] Intracellular signal transduction during mechano-response in differentiating chondrocytes. (2009)
  • Author(s): Takahashi I, Masuda T, Kohsaka K, Terao F, Anada T, Sasano Y, Takano-Yamamoto T, Suzuki O
  • Organizer: JSPS-KOSEF Asian Core Program, Nano/Amorphous Materials and Interface Science Symposium
  • Place of Presentation: Tohgatta, Miyagi.
  • Year and Date: 2009-08-07
• [Book] Molecular mechanisms of the response to mechanical stimulation during chondrocyte differentiation. Interface Oral Health Science 2009 (2010)
  • Author(s): Takahashi I, Masuda T, Kohsaka K, Terao F, Anada T, Sasano Y, Takano-Yamamoto T, Suzuki O
  • Publisher: Springer,New York
• [Book] Molecular mechanisms of the response to mechanical stimulation during chondrocyte differentiation. Interface Oral Health Science 2009 (2010)
  • Author(s): Takahashi I, Masuda T, Kohsaka K, Terao F, Anada T, Sasano Y, Takano-Yamamoto T Suzuki O.
  • Publisher: Springer, New York
• [Remarks]
  • URL: http://www.ortho.dent.kyushu-u.ac.jp/