| Project/Area Number |
21592588
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
|
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| Research Institution | Tohoku University |
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Principal Investigator |
IGARASHI Koaoru 東北大学, 大学院・歯学研究科, 教授 (70202851)
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| Co-Investigator(Kenkyū-buntansha) |
HARUYAMA Naoto 東京医科歯科大学, 歯と骨のGCOE拠点, GCOE拠点形成特任教員 (70359529)
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| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | アメロジェニン / エナメルタンパク / 骨代謝 / エストロゲン / 歯学 |
|---|
| Research Abstract |
Recent studies suggest that the amelogenins are expressed in various tissues including bone and affecting bone metabolism. In this study, we examined the effects of LRAP, one of the major splice isoform of amelogenins, on stimulated bone turnover under estrogen deficiency. Using mesenchymal cells isolated from wild type and LRAP transgenic mice with or without ovariectomy(OVX), gene expression levels of inter leukin-6(IL-6), cycrooxigenase-2(COX2), and nitric oxide (NO)synthase were examined by real-time PCR. In addition, ELISA or colorimetric assay was also performed to measure prostaglandin E_2(PGE_2)or NO production from the cells. We found that the LRAP inhibited the elevation of IL-6 and COX2 gene expression, which is typically found in the estrogen deficient condition. PGE_2 production was decreased in the ovariectomized LRAP TG derived cells, but not in the ovariectomized wild type derived cells. The production of NO was increased in the ovariectomized LRAP TG cells. The LRAP may play a significant role on the prevention from bone loss due to estrogen deficiency, by changing the interleukin and PGE_2 expression, and the production on NO
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