Mechanical response in artificially controlled osteocyte network

• Project/Area Number: 21592594
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Okayama University
• Principal Investigator: KAMIOKA Hiroshi 岡山大学, 大学院・医歯薬学総合研究科, 准教授 (80253219)
• Co-Investigator(Kenkyū-buntansha): SUGAWARA Yasuyo 岡山大学, 大学院・医歯薬学総合研究科, 助教 (70379775) ; KATANOSAKA Yuki 岡山大学, 大学院・医歯薬学総合研究科, 助教 (60432639)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 骨細胞 / ナノパターニング / 機械的刺激 / メカニカルストレス / 細胞間コミュニケーション

Research Abstract

Osteocyte in bone are thought to be mechanosensory cells. They form cellular network. However, the role of osteocyte network is not well known. We employed the nano-patterning method to form artificially-formed osteocyte network and applied mechanical stress with fluid shear stress in micro-fluid flow chamber.

Research Products

• [Journal Article] In situ imaging of the autonomous intracellular Ca2+ oscillations of osteoblasts and osteocytes in bone (2012)
  • Author(s): Ishihara Y, Sugawara Y, Kamioka H, Kawanabe N, Kurosaka H, Naruse K, Yamashiro T
  • Journal Title: Bone Volume: 50(4) Pages: 842-52
  • Peer Reviewed
• [Journal Article] 骨細胞による機械的刺激の感知 (2012)
  • Author(s): 上岡寛、山城隆
  • Journal Title: Clinical Calcium Volume: 21巻5号 Pages: 97-104
  • Peer Reviewed
• [Journal Article] Roles of heparan sulfate sulfation in dentinogenesis (2012)
  • Author(s): Hayano H, Kurosaka H, Yanagita T, Kalus I, Fabian Milz, Ishihara Y, Islam MN, Kawanabe N, Saito M, Kamioka H, Adachi T, Dierks T, Yamashiro T
  • Journal Title: J Biol Chem Volume: 287(15) Pages: 12217-29
  • Peer Reviewed
• [Journal Article] Stage-specific embryonic antigen-4 identifies human dental pulp stem cells (2012)
  • Author(s): Kawanabe N, Murata S, Fukushima H, Ishihara Y, Yanagita E, Ono M, Kurosaka H, Kamioka H, Itoh T, Kuboki T, Yamashiro T
  • Journal Title: Exp Cell Res Volume: 318(5) Pages: 453-463
  • Peer Reviewed
• [Journal Article] A case report of a patient with nonsyndromic bilateral and multiple impacted teeth and dentigerous cysts (2012)
  • Author(s): Ishihara Y, Kamioka H, Takano-Yamamoto T, Yamashiro T
  • Journal Title: American Journal of Orthodontics and Dentofacial Orthopedics Volume: 141(2) Pages: 228-241
  • Peer Reviewed
• [Journal Article] Intradermal injection of botulinum toxin type A alleviates infraorbital nerve constriction-induced thermal hyperalgesia in an operant assay (2012)
  • Author(s): Kumada A, Matsuka Y, Spigelman I, Maruhama K, Yamamoto Y, Neubert JK, Nolan TA, Watanabe K, Maekawa K, Kamioka H, Yamashiro T, Kuboki T, Oguma K
  • Journal Title: J Oral Rehabilitation Volume: 39(1) Pages: 63-72
  • Peer Reviewed
• [Journal Article] Sox9 expression during fracture repair (2011)
  • Author(s): Shintaku Y, Murakami T, Yanagita T, Kawanabe N, Fukunaga T, Matsuzaki K, Matsuoka S, Yoshida Y, Kamioka H, Takano-Yamamoto T, Takada K, Yamashiro T
  • Journal Title: Cells Tissues Organs Volume: 194(1) Pages: 38-48
  • Peer Reviewed
• [Journal Article] The three-dimensional morphometry and cell-cell communication of osteocytes network in chick and mouse embryonic calvaria (2011)
  • Author(s): Sugawara Y, Ando R, Kamioka H, Ishihara Y, Honjo T, Kawanabe N, Kurosaka H, Takano-Yamamoto T, Yamashiro T
  • Journal Title: Calcified Tissue International Volume: 88(5) Pages: 416-424
  • Peer Reviewed
• [Journal Article] Sox9 expression during fracture repair (2011)
  • Author(s): Shintaku Y, Murakami T, Yanagita T, et al
  • Journal Title: Cells Tissues Organs Volume: 194 Pages: 38-48
  • Peer Reviewed
• [Journal Article] The three-dimensional morphometry and cell-cell communication of osteocytes network in chick and mouse embryonic calvaria (2011)
  • Author(s): Sugawara Y, Ando R, Kamioka H, et al
  • Journal Title: Calcified Tissue International Volume: 88 Pages: 416-424
  • Peer Reviewed
• [Journal Article] Intradermal injection of botulinum toxin type A alleviates infraorbital nerve constriction-induced thermal hyperalgesia in an operant assay (2011)
  • Author(s): Kumada A, Matsuka Y, Spigelman I, et al
  • Journal Title: J Oral Rehabilitation Volume: 39 Pages: 63-72
  • Peer Reviewed
• [Journal Article] Application of bioimaging to osteocyte biology (2010)
  • Author(s): Kamioka H, Adachi T
  • Journal Title: Clinical Review in Bone and Mineral Metabolism Volume: 8(4) Pages: 170-178
  • Peer Reviewed
• [Journal Article] Isolation of multipotent stem cells in human periodontal ligament using stage-specific embryonic antigen-4 (2010)
  • Author(s): Kawanabe N, Murata S, Murakami K, Ishihara Y, Hayano S, Kurosaka H, Kamioka H, Takano-Yamamoto T, Yamashiro T
  • Journal Title: Differentiation Volume: 79 Pages: 74-83
  • Peer Reviewed
• [Journal Article] Calcium response in single osteocytes to locally applied mechanical stimulus : Differences in cell processes and cell body (2009)
  • Author(s): Adachi T, Aonuma Y, Tanaka M, Hojo M, Takano-Yamamoto T, Kamioka H
  • Journal Title: Journal of Biomechanics 42 Pages: 1989-1995
  • NAID: 120001596913
  • Peer Reviewed
• [Journal Article] A method for observing silver-stained osteocytes in situ in 3-micron sections using ultra-high voltage electron microscopy tomography (2009)
  • Author(s): Kamioka H, Murshid SA, Ishihara Y, Kajimura N, Hasegawa T, Ando R, Sugawara Y, Yamashiro T, Takaoka A, Takano-Yamamoto T
  • Journal Title: Microscopy and Microanalysis 15 Pages: 377-383
  • Peer Reviewed
• [Journal Article] Osteocyte Calcium Signaling Response to Bone Matrix Deformation (2009)
  • Author(s): Adachi T, Aonuma Y, Ito S, Tanaka M, Hojo M, Takano-Yamamoto T, Kamioka H
  • Journal Title: Journal of Biomechanics 42 Pages: 2507-2512
  • Peer Reviewed
• [Journal Article] Asymmetric intercellular communication between bone cells : Propagation of the calcium signaling (2009)
  • Author(s): Adachi T, Aonuma Y, Taira K, Hojo M, Kamioka H
  • Journal Title: Biochemical Biophysiology Research Communications 389 Pages: 495-500
  • Peer Reviewed
• [Journal Article] Sox9 expression during fracture repair
  • Author(s): Shintaku Y, Murakami T, Yanagita T, Kawanabe N, Fukunaga T, Matsuzaki K, Matsuoka S, Yoshida Y, Kamioka H, Takano-Yamemoto T.Takada K, Yamashiro T.
  • Journal Title: Cells Tissues Organs Volume: (掲載確定)
  • Peer Reviewed
• [Presentation] 三次元再構築された骨細管内での流れ解析の試み (2011)
  • Author(s): 上岡寛
  • Organizer: 大阪大学超高圧電子顕微鏡センター医学・生物学系共同利用者研究報告会
  • Place of Presentation: 大阪市
  • Year and Date: 2011-11-22
• [Presentation] 骨細胞のバイオイメージングによる機能解析 (2011)
  • Author(s): 上岡寛
  • Organizer: 京都大学再生医科学研究所第8回バイオメカニクスセミナー
  • Place of Presentation: 京都市
  • Year and Date: 2011-11-16
• [Presentation] 骨細胞のイメージングによる機能解析 (2011)
  • Author(s): 上岡寛
  • Organizer: 国立長寿医療研究センター運動器疾患研究部主催セミナー
  • Place of Presentation: 大府市
  • Year and Date: 2011-10-27
• [Presentation] 骨細胞のイメージングによる機能解析 (2011)
  • Author(s): 上岡寛
  • Organizer: 国立長寿医療研究センター運動器疾患研究部主催セミナー(竹下淳室長)
  • Place of Presentation: 大府市
  • Year and Date: 2011-10-27
• [Presentation] マウス大腿骨中の三次元骨細胞ネットワークは外部環境によって変化する (2011)
  • Author(s): 菅原康代、上岡寛、石原嘉人、藤澤直子、山城隆
  • Organizer: 第70回日本矯正歯科学会大会 & 第4回国際会議
  • Place of Presentation: 名古屋市
• [Presentation] 生体ライブイメージングを用いた骨組織中における自律性細胞内カルシウムオシレーションの検討 (2011)
  • Author(s): 石原嘉人、菅原康代、上岡寛、川邉、山城隆
  • Organizer: 第70回日本矯正歯科学会大会 & 第4回国際会議
  • Place of Presentation: 名古屋市
• [Presentation] Microstructure of osteocyte cell processes and surrounding bone matrix revealed by ultra-high voltage electron microscopy tomography (2010)
  • Author(s): Kamioka H, Imai Y, Bakker AD, Bacabac RG, Yamashiro T, Klein-Nulend J
  • Organizer: 11th International Bone Fluid Flow Workshop
  • Place of Presentation: トロント、カナダ
  • Year and Date: 2010-10-14
• [Presentation] Direct Imaging of Flow-induced Oscillatory Calcium Rise in Living Bone (2010)
  • Author(s): Ishihara Y, Sugawara Y, Kamioka H, Honjo T, Kurosaka H, Kawanabe N, Yamashiro T
  • Organizer: JADR Hatton award competition
  • Place of Presentation: Tokyo
  • Year and Date: 2010-01-25
• [Presentation] ヒト歯根膜細胞におけるギャップ結合を介した細胞間コミュニケーション (2010)
  • Author(s): 吉川祐介、石原嘉人、今井裕一、川邉紀章、上岡寛、山城隆
  • Organizer: 第69回日本矯正歯科学会大会
  • Place of Presentation: 横浜市
• [Presentation] 骨組織内ライブイメージングを用いた機械的刺激に対する細胞内カルシウム応答の検討 (2010)
  • Author(s): 石原嘉人, 菅原康代, 上岡寛, 川邉紀章, 成瀬恵治, 山城隆
  • Organizer: 第69回日本矯正歯科学会大会
  • Place of Presentation: 横浜市
• [Presentation] 力学刺激に対する単離骨細胞の情報伝達特性に関する考察 (2010)
  • Author(s): 青沼有紀、平圭佑、安達泰治、北條正樹、上岡寛
  • Organizer: 第22回バイオエンジニアリング講演会
  • Place of Presentation: 岡山市
• [Presentation] Observation of Calcium Signaling Response of Osteocytes to Bone Matrix Deformation (2009)
  • Author(s): Adachi T, Aonuma Y, Ito S, Tanaka M, Hojo M, Takano-
  • Organizer: Third Switzerland-Japan workshop on Biomechanics 2009
  • Place of Presentation: Engelberg, Switzerland
• [Presentation] 単離骨系細胞におけるカルシウム応答伝搬の非対称性 (2009)
  • Author(s): 平圭佑、青沼有紀、安達泰治、北條正樹、上岡寛
  • Organizer: 日本機械学会2009年度年次大会
  • Place of Presentation: 盛岡市
• [Patent(Industrial Property Rights)] 細胞培養システム (2011)
  • Inventor(s): 上岡寛, 他
  • Industrial Property Rights Holder: 上岡寛, 他
  • Filing Date: 2011-10-04