Analysis of tooth movement mechanism in TNF receptors deficient chimera mouse

• Project/Area Number: 21592607
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Tohoku University
• Principal Investigator: KITAURA Hideki 東北大学, 病院, 講師 (60295087)
• Co-Investigator(Kenkyū-buntansha): YOSHIMATSU Masako 長崎大学, 医学部・歯学部附属病院, 助教 (20420630) ; MASUYAMA Ritsuko 長崎大学, 大学院・医歯薬学総合研究科, 助教 (60297596) ; FUJIMURA Yuji 長崎大学, 大学院・医歯薬学総合研究科, 助教 (70448504)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 歯科矯正学 / 破骨細胞 / TNF-α / 骨髄細胞 / 骨髄移植 / T細胞 / 矯正歯科 / マウス / キメラマウス

Research Abstract

We generated chimeric mice using wild type (WT)or TNF receptor deficient (KO)marrow, immunodepleted of T-cells and stromal cells. The samples were reciprocally transplanted into WT or KO mice following lethal irradiation to eliminate native marrow. We established an orthodontic tooth movement model in mice by using a Ni-Ti closed coil spring. We found that orthodontic tooth movement was occurred in chimeric mice.

Research Products

• [Journal Article] Inhibitory effects of IL-12 on experimental tooth movement and root resorption in mice (2012)
  • Author(s): Yoshimatsu, M., Kitaura, H., Fujimura, Y., Kohara, H., Morita, Y., Eguchi, T., Yoshida, N
  • Journal Title: Arch. Oral Biol Volume: 57, 1 Pages: 36-43
  • Peer Reviewed
• [Journal Article] Anti-c-Fms antibody inhibits lipopolysaccharide-induced osteoclastogenesis in vivo (2012)
  • Author(s): Kimura, K., Kitaura, H., Fujii, T., Hakami Z., Takano-Yamamoto T
  • Journal Title: FEMSImmunol. Med. Microbiol Volume: 64, 2 Pages: 219-227
  • Peer Reviewed
• [Journal Article] Comparative Proteomic Analysis of a Cytosolic Fraction from β 3 Integrin-deficient Cells (2012)
  • Author(s): Bush, JA., Kitaura, H., Ma, Y., Teitelbaum, SL., Ross, FP., Smith JW.
  • Journal Title: Cancer Genomics Proteomics Volume: 9, 1 Pages: 1-13
  • Peer Reviewed
• [Journal Article] IL-12-and L-18-mediated nitric oxide induced apoptosis of adherent bone marrow cells in TNF-a-induced osteoclast formation (2012)
  • Author(s): Kitaura, H., Aonuma, T., Fukumoto, E., Kimura, K., Fujii, T., Hakami, Z., Takano-Yamamoto T
  • Journal Title: Interface Oral Health Science 2011 Pages: 125-127
• [Journal Article] Inhibitory effects of IL-12 on experimental tooth movement and root resorption in mice (2012)
  • Author(s): Yoshimatsu, M., et.al
  • Journal Title: Arch.Oral Biol. Volume: 57 Pages: 36-43
  • Peer Reviewed
• [Journal Article] Anti-c-Fms antibody inhibits lipopolysaccharide-induced osteoclastogenesis in vivo (2012)
  • Author(s): Kimura, K., et.al
  • Journal Title: FEMS Immunol.Med.Microbiol. Volume: 64 Pages: 219-227
  • Peer Reviewed
• [Journal Article] Comparative Proteomic Analysis of a Cytosolic Fraction from β3 Integrin-deficient Cells (2012)
  • Author(s): Bush, JA., et.al
  • Journal Title: Cancer Genomics Proteomics Volume: 9 Pages: 1-13
  • Peer Reviewed
• [Journal Article] Calcium/calmodulin-signaling supports TRPV4 activation in osteoclasts and regulates bone mass (2012)
  • Author(s): Masuyama R, et.al
  • Journal Title: J.Bone Miner.Res. Volume: (in press)
  • Peer Reviewed
• [Journal Article] IL-12- and IL-18-mediated nitric oxide induced apoptosis of adherent bone marrow cells in TNF-a-induced osteoclast formation (2012)
  • Author(s): Kitaura, H., et.al
  • Journal Title: Interface Oral Health Science 2011 Pages: 125-127
• [Journal Article] IFN-γ directly inhibits TNF-α-induced osteoclastogenesis in vitro and in vivo and induces apoptosis mediated by Fas/Fas ligand interaction (2011)
  • Author(s): Kohara, H., Kitaura, H., Fujimura, Y., Yoshimatsu, M., Morita, Y., Eguchi, T., Masuyama, R., Yoshida, N.
  • Journal Title: Immunol. Lett Volume: 137, 1-2 Pages: 53-61
  • Peer Reviewed
• [Journal Article] IL-12-and IL-18-mediated nitric oxide-induced apoptosis in TNF-α-mediated osteoclastogenesis of bone marrow cells (2011)
  • Author(s): Kitaura, H., Fujimura, Y., Yoshimatsu, M., Kohara, H., Morita, Y., aonuma, T., Fukumoto, E., Masuyama, R., Yoshida, N., Takano-Yamamoto T
  • Journal Title: Calcif. Tissue Int Volume: 89, 1 Pages: 65-73
  • Peer Reviewed
• [Journal Article] Overexpression of bcl2 in osteoblasts inhibits osteoblast differentiation and induces osteocyte apoptosis (2011)
  • Author(s): Moriishi, T., Maruyama, Z., Fukuyama, R., Ito, M., Miyazaki, T., Kitaura, H., Ohnishi, H., Furuichi, T., Kawai, Y., Masuyama, R., Komori, H., Takada, K., Kawaguchi, H., Komori, T
  • Journal Title: PLoS One Volume: 6, 11
  • NAID: 120006985216
  • Peer Reviewed
• [Journal Article] TNF-αの骨代謝作用 (2011)
  • Author(s): 北浦英樹
  • Journal Title: 骨粗鬆症治療 Volume: 10, 1 Pages: 10-14
• [Journal Article] 特集カラーレビュー骨髄細胞でのTNF-aによる破骨細胞形成におけるIL-12 およびIL-18 によるアポトーシス誘導 (2011)
  • Author(s): 北浦英樹
  • Journal Title: 骨粗鬆症治療 Volume: 10, 1 Pages: 8-8
• [Journal Article] IFN-γ directly inhibits TNF-α-induced osteoclastogenesis in vitro and in vivo and induces apoptosis mediated by Fas/Fas ligand interaction (2011)
  • Author(s): Kohara, H., et.al.
  • Journal Title: Immunol.Lett. Volume: 137 Pages: 53-61
  • Peer Reviewed
• [Journal Article] IL-12- and IL-18-mediated nitric oxide-induced apoptosis in TNF-α-mediated osteoclastogenesis of bone marrow cells (2011)
  • Author(s): Kitaura, H., et.al
  • Journal Title: Calcif.Tissue Int. Volume: 89 Pages: 65-73
  • Peer Reviewed
• [Journal Article] Overexpression of bcl2 in osteoblasts inhibits osteoblast differentiation and induces osteocyte apoptosis (2011)
  • Author(s): Moriishi, T., et.al
  • Journal Title: PLoS One. Volume: 6
  • Peer Reviewed
• [Journal Article] デンタルインプラントを利用して矯正治療を行った成人反対咬合症例 (2011)
  • Author(s): 江口俊子, 他
  • Journal Title: 九矯歯誌 Volume: 7 Pages: 23-32
  • Peer Reviewed
• [Journal Article] 骨髄細胞でのTNF-aによる破骨細胞形成におけるIL-12およびIL-18によるアポトーシス誘導 (2011)
  • Author(s): 北浦英樹
  • Journal Title: 骨粗鬆症治療 Volume: 10 Pages: 8-8
• [Journal Article] 機能的矯正装置における構成咬合について (2011)
  • Author(s): 北浦英樹
  • Journal Title: 小児歯科臨床 Volume: 16 Pages: 49-54
• [Journal Article] IFN-γ directly inhibits TNF-α-induced osteoclastogenesis in vitro and in vivo and induces apoptosis mediated by Fas/Fas ligand interaction. (2011)
  • Author(s): Kohara, H., et.al
  • Journal Title: Immunol.Lett. Volume: 未定(in press)
  • Peer Reviewed
• [Journal Article] TNF-αの骨代謝作用 (2011)
  • Author(s): 北浦英樹
  • Journal Title: 骨粗霧症治療 Volume: 未定(印刷中)
• [Journal Article] IL-18 Inhibits TNF-a-induced osteoclastogenesis possibly via a T cell-independent mechanism in synergy with IL-12 in vivo (2010)
  • Author(s): Morita, Y., Kitaura, H., Yoshimatsu, M., Fujimura, Y., Kohara, H., Eguchi, T., Yoshida, N
  • Journal Title: Calcif. TissueInt Volume: 86, 3 Pages: 242-248
  • Peer Reviewed
• [Journal Article] IL-18 Inhibits TNF-a-induced osteoclastogenesis possibly viaa T cell-independent mechanism in synergy with IL-12 in vivo. (2010)
  • Author(s): Morita, Y., et. al
  • Journal Title: Calcif.Tissue Int. Volume: 86 Pages: 242-248
  • Peer Reviewed
• [Journal Article] 上顎左側側切歯欠損, 上下顎叢生, 正中離開を伴う片側性唇顎口蓋裂症例 (2010)
  • Author(s): 北浦英樹, 他
  • Journal Title: 九矯歯誌 Volume: 6 Pages: 87-94
  • Peer Reviewed
• [Journal Article] A M-CSF receptor c-Fms antibody inhibits mechanical stress-induced root resorption during orthodontic tooth movement in mice (2009)
  • Author(s): Kitaura, H., Fujimura, Y., Yoshimatsu, M., Eguchi, T., Kohara, H., Insan, J., Morita, Y., Yoshida, N
  • Journal Title: Angle Orthod Volume: 79, 5 Pages: 835-841
  • Peer Reviewed
• [Journal Article] Influence of bisphosphonates on orthodontic tooth movement in mice (2009)
  • Author(s): Fujimura, Y., Kitaura, H., Yoshimatsu, M., Eguchi, T., Kohara, H., Morita, Y., Yoshida, N.
  • Journal Title: Eur. J. Orthod Volume: 31, 6 Pages: 572-577
  • Peer Reviewed
• [Journal Article] IL-12 inhibits TNF-α induced osteoclastogenesis via a T cell-independent mechanism in vivo (2009)
  • Author(s): Yoshimatsu, M., Kitaura, H., Fujimura, Y., Eguchi, T., Kohara, H., Morita, Y., Yoshida, N.
  • Journal Title: Bone Volume: 45, 5 Pages: 1010-1016
  • Peer Reviewed
• [Journal Article] A M-CSF receptor c-Fms antibody inhibits mechanical stress-induced root resorption during orthodontic tooth movement in mice. (2009)
  • Author(s): Kitaura, H., et.al
  • Journal Title: Angle Orthod. 79 Pages: 835-841
  • Peer Reviewed
• [Journal Article] Influence of bisphosphonates on orthodontic tooth movement in mice. (2009)
  • Author(s): Fujimura, Y., et.al
  • Journal Title: Eur.J.Orthod. 31 Pages: 572-577
  • Peer Reviewed
• [Journal Article] IL-12 inhibits TNF-α induced osteoclastogenesis via a T cell-independent mechanism in vivo. (2009)
  • Author(s): Yoshimatsu, M., et.al
  • Journal Title: Bone. 45 Pages: 1010-1016
  • Peer Reviewed
• [Journal Article] Calcium/calmodulin-signaling supports TRPV4 activation in osteoclasts and regulates bone mass
  • Author(s): Masuyama R, Mizuno A, Komori H, Kajiya H, Uekawa A, Kitaura H, Okabe K, Ohyama K, Komori T
  • Journal Title: J. Bone Miner. Res Volume: (in press)
  • Peer Reviewed
• [Presentation] LPS誘導病的骨吸収に対するM-CSF中和抗体の作用の検討 (2012)
  • Author(s): 木村桂介, 他
  • Organizer: 第33回東北骨代謝研究会
  • Place of Presentation: 仙台
  • Year and Date: 2012-02-04
• [Presentation] 東北大学および宮城県における震災後の矯正歯科患者の実態調査 (2011)
  • Author(s): 北浦英樹
  • Organizer: 第5回東北矯正歯科学会秋期セミナー、シンポジウム:東日本大震災における子ども達の矯正歯科からみた現状と課題
  • Place of Presentation: 仙台(招待講演)
  • Year and Date: 2011-09-19
• [Presentation] IL-4のTNF-αによる破骨細胞形成に対する抑制効果についてのin vivoでの検討 (2011)
  • Author(s): 藤井俊哉, 他
  • Organizer: 59回東北大学歯学会
  • Place of Presentation: 仙台
  • Year and Date: 2011-06-10
• [Presentation] IL-12- and IL-18-mediated nitric oxide induced apoptosis of adherent bone marrow cells in TNF-α-induced osteoclast formation. (2011)
  • Author(s): Kitaura H, et.al
  • Organizer: The 4th International Symposium for Interface Oral Heaith Science in Sendai
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-08
• [Presentation] Interleukin-4 inhibits tumor necrosis factor-α-mediated osteoclast formation in vitro and in vivo. (2011)
  • Author(s): Fujii T, et.al
  • Organizer: The 4th International Symposium for Interface Oral Health Science in Sendai
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-08
• [Presentation] IL-4のTNF-αによる破骨細胞形成への影響のin vivoでの検討 (2011)
  • Author(s): 藤井俊哉, 他
  • Organizer: 第32回東北骨代謝研究会
  • Place of Presentation: 仙台
  • Year and Date: 2011-02-05
• [Presentation] Effect of SHIP on TNF-alpha-induced osteoclastogenesis (2011)
  • Author(s): Yoshimatsu M, Kitaura H, Fujimura Y, Kohara H, Morita Y, Eguchi T, Yoshida N.
  • Organizer: The 4th International Congress and The 70th Annual Meeting of the Japanese Orthodontic Society
  • Place of Presentation: Nagoya, Japan
• [Presentation] Anti-c-Fms antibody inhibits lipopolysaccharide-induced osteoclastogenesis in vivo (2011)
  • Author(s): Kimura, K., Kitaura, H., Fujii, T., Hakami Z., Takano-Yamamoto T
  • Organizer: The 4th International Congress and The 70th Annual Meeting of the Japanese Orthodontic Society
  • Place of Presentation: Nagoya, Japan
• [Presentation] Interleukin-4 inhibits tumor necrosis factor-α-mediated osteoclast formation in vivo (2011)
  • Author(s): Fujii T, Kitaura H, Kimura K, Hakami Z, Takano-Yamamoto T
  • Organizer: The 4th International Congress and The 70th Annual Meeting of the Japanese Orthodontic Society
  • Place of Presentation: Nagoya, Japan
• [Presentation] IL-12-and IL-18-mediated nitric oxide-induced apoptosis in TNF-α-mediated osteoclastogenesis (2011)
  • Author(s): Kitaura H, Fujimura Y, Yoshimatsu M, Kohara H, Morita Y, Aonuma T, Fukumoto E, Yoshida N, Takano-Yamamoto T
  • Organizer: The 4th International Congress and The 70th Annual Meeting of the Japanese Orthodontic Society
  • Place of Presentation: Nagoya, Japan
• [Presentation] IL-12-and IL-18-mediated nitric oxide induced apoptosis of adherent bone marrow cells in TNF-α-induced osteoclast formation (2011)
  • Author(s): Kitaura H, Aonuma T, Fukumoto E, Kimura K, Fujii T, Hakami Z, Takano-Yamamoto T
  • Organizer: The 4th International Symposium for Interface Oral Health Science in Sendai
  • Place of Presentation: Sendai, Japan
• [Presentation] IL-12- and IL-18-mediated nitric oxide induced apoptosis of adherent bone marrow cells in TNF-α-induced osteoclast formation (2011)
  • Author(s): Kitaura, H., et.al
  • Organizer: The 4th International Symposium for Interface Oral Health Science in Sendai
  • Place of Presentation: 仙台
• [Presentation] Interleukin-4 inhibits tumor necrosis factor-α-mediated osteoclast formation in vitro and in vivo (2011)
  • Author(s): Fujii, T., et.al
  • Organizer: The 4th International Symposium for Interface Oral Health Science in Sendai
  • Place of Presentation: 仙台
• [Presentation] IL-12- and IL-18-mediated nitric oxide-induced apoptosis in TNF-α-mediated osteoclastogenesis (2011)
  • Author(s): Kitaura, H., et.al
  • Organizer: The 4th International Congress and The 70th Annual Meeting of the Japanese Orthodontic Society
  • Place of Presentation: 名古屋
• [Presentation] Anti-c-Fms antibody inhibits lipopolysaccharide-induced osteoclastogenesis in vivo (2011)
  • Author(s): Kimura, K., et.al
  • Organizer: The 4th International Congress and The 70th Annual Meeting of the Japanese Orthodontic Society
  • Place of Presentation: 名古屋
• [Presentation] Effect of IFN-γ on orthodontic tooth movement (2011)
  • Author(s): Kohara, H., et.al
  • Organizer: The 4th International Congress and The 70th Annual Meeting of the Japanese Orthodontic Society
  • Place of Presentation: 名古屋
• [Presentation] Effect of SHIP on TNF-alpha-induced osteoclastogenesis (2011)
  • Author(s): Yoshimatu, M., et.al
  • Organizer: The 4th International Congress and The 70th Annual Meeting of the Japanese Orthodontic Society
  • Place of Presentation: 名古屋
• [Presentation] IFN-γによるTNF-α誘導性破骨細胞の形成抑制効果 (2011)
  • Author(s): 小原悠, 他
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
• [Presentation] 上顎左側中切歯および側切歯の萌出遅延に対し開窓・牽引を行った1症例 (2011)
  • Author(s): 藤村裕治, 他
  • Organizer: 第70回日本矯正歯科学会大会&第4回国際会議
  • Place of Presentation: 名古屋
• [Presentation] イオンプレーティング法による矯正用金属コーティングの耐食性に関する検討 (2010)
  • Author(s): 北浦英樹, 他
  • Organizer: 第69回日本矯正歯科学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2010-09-29
• [Presentation] 矯正学的歯の移動時の歯根吸収におけるIL-12の作用について (2010)
  • Author(s): 吉松昌子, 他
  • Organizer: 第69回日本矯正歯科学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2010-09-29
• [Presentation] IL-18のTNF-α誘導破骨細胞形成抑制作用におけるT細胞の関与の検討 (2010)
  • Author(s): 森田幸子, 他
  • Organizer: 第69回日本矯正歯科学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2010-09-29
• [Presentation] 矯正学的歯の移動に対するIFN-γの発現およびその影響 (2010)
  • Author(s): 小原悠
  • Organizer: 第69回日本矯正歯科学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2010-09-29
• [Presentation] バイオネーターにおける構成咬合 (2010)
  • Author(s): 北浦英樹
  • Organizer: 第2回機能的顎治療を考える会
  • Place of Presentation: 郡山
  • Year and Date: 2010-07-03
• [Presentation] 咬筋切除術を行ったAngle II級2類症例 (2010)
  • Author(s): 鈴木章裕, 他
  • Organizer: 第26回東北矯正歯科学会大会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-05-23
• [Presentation] IL-12- and IL-18-mediated Nitric Oxide Induces Apoptosis in TNF-α-induced Osteoclastogenesis of Bone Marrow Cells (2009)
  • Author(s): Kitaura, H., et.al
  • Organizer: The 31st Annual Meeting of the American Society for Bone and Mineral Research.
  • Place of Presentation: 米国 デンバー
  • Year and Date: 2009-09-15
• [Presentation] IL-18 inhibits TNF-a-induced osteoclastogenesis possibly via a T cell-independent mechanism in synergy with IL-12 in vitro and in vivo (2009)
  • Author(s): Morita Y, Kitaura H, Kohara H, Fujimura Y, Yoshimatsu M, Eguchi T, Yoshida N
  • Organizer: The 6th Japan-Korea conference on cellular signaling for young scientists
  • Place of Presentation: Nagasaki, Japan
• [Presentation] IFN-γ. mediated nitric oxide inhibits proliferation of M-CSF. dependent bone marrow macrophages (2009)
  • Author(s): Kohara H, Kitaura H, Fujimura Y, Yoshimatsu M, Morita Y, Eguchi T, Takano-Yamamoto T, Yoshida N
  • Organizer: The 31st Annual Meeting of the American Society for Bone and Mineral Research.Denver
  • Place of Presentation: U. S. A
• [Presentation] IL-18 inhibits TNF-α-induced osteoclastogenesis in synergy with IL-12 in vivo (2009)
  • Author(s): Morita Y, Kitaura H, Fujimura Y, Yoshimatsu M, Kohara H, Eguchi T, Takano-Yamamoto T, Yoshida N
  • Organizer: The 31st Annual Meeting of the American Society for Bone and Mineral Research.Denver
  • Place of Presentation: U. S. A
• [Presentation] Takano-Yamamoto T, Yoshida N. Effect of Bisphosphonates on Orthodontic Tooth Movement in Mice (2009)
  • Author(s): Fujimura Y, Kitaura H, Yoshimatsu M, Kohara H, Morita Y, Eguchi T
  • Organizer: The 31st Annual Meeting of the American Society for Bone and Mineral Research.Denver
  • Place of Presentation: U. S. A
• [Presentation] IL-12 inhibits TNF-α mediated osteoclastogenesis via a T cell-independent mechanism in vivo (2009)
  • Author(s): Yoshimatsu M, Kitaura H, Fujimura Y, Kohara H, Morita Y, Eguchi T, Takano-Yamamoto T, Yoshida N
  • Organizer: The 31st Annual Meeting of the American Society for Bone and Mineral Research.Denver
  • Place of Presentation: U. S. A
• [Presentation] IL-12-and IL-18-mediated Nitric Oxide Induces Apoptosis in TNF-α-induced Osteoclastogenesis of Bone Marrow Cells (2009)
  • Author(s): Kitaura H, Fujimura Y, Yoshimatsu M, Kohara H, Morita Y, Eguchi T, Yoshida N, Takano-Yamamoto T
  • Organizer: The 31st Annual Meeting of the American Society for Bone and Mineral Research.Denver
  • Place of Presentation: U. S. A