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Investigation of stretch-induced intracellular signal transduction in human periodontal ligament fibroblasts exposed to periodontopathic factors.

Research Project

Project/Area Number 21592617
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthodontic/Pediatric dentistry
Research InstitutionKagoshima University (2010-2011)
Asahi University (2009)

Principal Investigator

YAGI Takakazu  鹿児島大学, 医学部・歯学部附属病院, 講師 (10346166)

Co-Investigator(Kenkyū-buntansha) MIYAWAKI Shouichi  鹿児島大学, 大学院・医歯学総合研究科, 教授 (80295807)
MATSUGUCHI Tetsuya  鹿児島大学, 大学院・医歯学総合研究科, 教授 (10303629)
INTO Takeshi  朝日大学, 歯学部, 講師 (10360918)
Co-Investigator(Renkei-kenkyūsha) KITAI Noriyuki  朝日大学, 歯学部, 教授 (20271025)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsメカニカルストレス / ヒト歯根膜細胞線維芽細胞 / 細胞内シグナル伝達機構 / 歯根膜細胞 / 炎症性シグナリングカスケード / 歯根膜線維芽細胞 / シグナリングカスケード
Research Abstract

In this study, we investigated the mechanism of intracellular signaling cascade with mechanical stress using the human periodontal ligament fibroblast(HPLF). And we investigated the cellular response of the active center of lipopolysaccharide(LPS) endotoxin which is a virulence factor of periodontal disease. Moreover, we researched the effect of mechanical stress against these cell receptors. As a result, we confirmed that HPLFs were expressed on the inflammatory cytokines via the MAPK signaling cascade in the uni-axial stretch. Next, we found that the expression of inflammatory cytokines through the MAPK signaling cascade was enhanced by the mechanical stress after stimulating by the virulence factors of periodontal disease.
Therefore, it is suggested that the cell receptor against the center of virulence of LPS might be affected by the mechanical deformation. Further study is required in the future.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (10 results)

All 2012 2011 2010

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (2 results) Book (2 results)

  • [Journal Article] Patient with oculo-facio-cardio-dental syndrome treated with surgical orthodontics2012

    • Author(s)
      Sakaguchi, K., Yagi, T., Nagata, J., Kubota, T., Sugihara, K. and Miyawaki, S., et al
    • Journal Title

      Am J Orthod Dentofacial Orthop

      Volume: 141(4 Suppl) Issue: 4 Pages: S159-S170

    • DOI

      10.1016/j.ajodo.2011.10.020

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Patient with oculo-fecio-cardio-dental syndrome treated with surgical orthodontics2012

    • Author(s)
      Sakaguchi K, Takakazu Y, Nagata J, Kubota T, Sugihara K, Miyawaki S
    • Journal Title

      Am J Orthod Dentofacial Orthop

      Volume: 141 Pages: 159-170

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Relationship between occlusal curvatures and bite force in humans2012

    • Author(s)
      Sakaguchi K, Uehara S, Yagi T, Miyawaki S
    • Journal Title

      Orthod Waves

      Volume: 71(in press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 咬合高径の維持に関する神経機構の研究2011

    • Author(s)
      八木孝和, 宮脇正一
    • Journal Title

      鹿歯紀要

      Volume: 31 Pages: 81-91

    • NAID

      120005231986

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bioactive mechanism of Porphyromonas gingivalis lipid A2010

    • Author(s)
      Ogawa, T. and Yagi, T.
    • Journal Title

      Periodontology 2000

      Volume: 54(1) Issue: 1 Pages: 71-7

    • DOI

      10.1111/j.1600-0757.2009.00343.x

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Bioactive mechanism of Porphyromonas gingivalis lipid A.2010

    • Author(s)
      Ogawa T, Yagi T
    • Journal Title

      Periodontology 2000

      Volume: 54 Pages: 71-77

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 歯周病原性因子存在下における培養ヒト歯根膜線維芽細胞への物理的刺激による細胞内シグナル伝達系の動態検討2011

    • Author(s)
      八木孝和, 前田綾, 植田紘貴, 菅真由, 宮脇正一
    • Organizer
      日本矯正歯科学会
    • Place of Presentation
      古屋市
    • Related Report
      2011 Final Research Report
  • [Presentation] 歯周病原性因子存在下における培養ヒト歯根膜線維芽細胞への物理的刺激による細胞内シグナル伝達系の動態検討Investigation of stretch-induced intracellular signal transduction in human periodontal ligament fibroblasts exposed to periodontopathic factors2011

    • Author(s)
      八木孝和、前田綾、植田紘貴、菅真有、宮脇正一
    • Organizer
      日本矯正歯科学会
    • Place of Presentation
      名古屋市
    • Related Report
      2011 Annual Research Report
  • [Book] 矯正治療の視点(歯周-矯正治療STOP & GO :伊藤公一,保田好隆編著)2012

    • Author(s)
      八木孝和, 友成博, 小山勲男, 宮脇正一
    • Publisher
      クインテッセンス出版
    • Related Report
      2011 Final Research Report
  • [Book] 矯正治療の視点(p50-66)(歯周-矯正治療STOP & GO:伊藤公一,保田好隆編著)2012

    • Author(s)
      八木孝和, 友成博, 小山勲男, 宮脇正一
    • Total Pages
      211
    • Publisher
      クインテッセンス出版
    • Related Report
      2011 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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