| Project/Area Number |
21592617
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Kagoshima University (2010-2011)
Asahi University (2009) |
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Principal Investigator |
YAGI Takakazu 鹿児島大学, 医学部・歯学部附属病院, 講師 (10346166)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAWAKI Shouichi 鹿児島大学, 大学院・医歯学総合研究科, 教授 (80295807)
MATSUGUCHI Tetsuya 鹿児島大学, 大学院・医歯学総合研究科, 教授 (10303629)
INTO Takeshi 朝日大学, 歯学部, 講師 (10360918)
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| Co-Investigator(Renkei-kenkyūsha) |
KITAI Noriyuki 朝日大学, 歯学部, 教授 (20271025)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | メカニカルストレス / ヒト歯根膜細胞線維芽細胞 / 細胞内シグナル伝達機構 / 歯根膜細胞 / 炎症性シグナリングカスケード / 歯根膜線維芽細胞 / シグナリングカスケード |
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| Research Abstract |
In this study, we investigated the mechanism of intracellular signaling cascade with mechanical stress using the human periodontal ligament fibroblast(HPLF). And we investigated the cellular response of the active center of lipopolysaccharide(LPS) endotoxin which is a virulence factor of periodontal disease. Moreover, we researched the effect of mechanical stress against these cell receptors. As a result, we confirmed that HPLFs were expressed on the inflammatory cytokines via the MAPK signaling cascade in the uni-axial stretch. Next, we found that the expression of inflammatory cytokines through the MAPK signaling cascade was enhanced by the mechanical stress after stimulating by the virulence factors of periodontal disease.
Therefore, it is suggested that the cell receptor against the center of virulence of LPS might be affected by the mechanical deformation. Further study is required in the future.
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