Specification and verification of periodontal pathogens relate to various vascular diseases

• Project/Area Number: 21592621
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Osaka Dental University (2011); Tokyo Medical and Dental University (2009-2010)
• Principal Investigator: UMEDA Makoto 大阪歯科大学, 歯学部, 教授 (90193937)
• Co-Investigator(Kenkyū-buntansha): INOUE Yoshinori 東京医科歯科大学, 大学院・医歯学総合研究科, 講師 (70280964)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 血管系疾患 / 歯周病原細菌 / モノクローナル抗体 / 免疫染色 / リンパ節 / バージャー病 / P. gingivalis / T. forsythia / 免疫二重染色 / 病的歯肉組織 / 動脈硬化 / リアルタイムPCR / アテローム性動脈硬化症 / P.gingivalis / T.forsythia

Research Abstract

The monoclonal antibodies effective for the detection of P. gingivalis and T. forsythia localized in tissue specimens were prepared. Periodontal pathogen was detected in diseased gingival tissue and atherosclerosis sample by immunohistochemistry. Oral and vascular samples of Buerger disease patients in Sri Lanka were detected periodontal pathogens by polymerase chain reaction method. The existence of periodontal pathogens in formalin-fixed and paraffin embedded lymph nodes proximal to oral cavity was examined from patients with histories of head and neck cancer. These bacteria invaded in gingival tissues might translocate peripheral arteries via lymph nodes.

Research Products

• [Journal Article] Clinical Study of Phlebitis Migrans and Incompetence of the Leg's Superficial Vein in Buerger Disease (2012)
  • Author(s): Iwai T, Sato S, Kume H, Inoue Y, Umeda M, Kagayama T, Hirokawa M
  • Journal Title: Annals of Vascular Diseases Volume: 5 Issue: 1 Pages: 45-51
  • DOI: 10.3400/avd.oa.11.00044
  • NAID: 130004695416
  • ISSN: 1881-641X, 1881-6428
  • Peer Reviewed
• [Journal Article] The periodontal anaerobe Porphyromonas gingivalis induced platelet activation and increased aggregation in whole blood by rat model (2011)
  • Author(s): Yu KM, Inoue Y, Umeda M, Terasaki H, Chen ZY, Iwai T
  • Journal Title: Tromb Res Volume: 127巻 Issue: 5 Pages: 418-425
  • DOI: 10.1016/j.thromres.2010.12.004
  • Peer Reviewed
• [Journal Article] Whole-blood platelet aggregation by Porphyromonas gingivalis in patients with peripheral arterial disease (2011)
  • Author(s): Notani H, Inoue Y, Sugano N, Jibiki M, Umeda M, Izumi Y
  • Journal Title: J Med Dent Sci Volume: 58巻 Pages: 7-14
  • NAID: 130006943751
  • URL: http://www.tmd.ac.jp/english/labs/toshozy
  • Peer Reviewed
• [Journal Article] Bactericidal effects of a high-power red light-emitting diode on two periodontopathic bacteria in antimicrobial photodynamic therapy in vitro (2011)
  • Author(s): Umeda M, Tsuno A, Okagami Y, Tsuchida F, Izumi Y, Ishikawa I
  • Journal Title: J Investig Clin Dent Volume: 2巻 Issue: 4 Pages: 268-274
  • DOI: 10.1111/j.2041-1626.2011.00071.x
  • Peer Reviewed
• [Journal Article] バージャー病患者の逍遙性静脈炎、表在静脈弁不全についての考察と新知見 (2011)
  • Author(s): 岩井武尚、佐藤彰治、久米博子、井上芳徳、梅田誠、加賀山知子、広川雅之
  • Journal Title: 静脈学 Volume: 22巻、No.1 Pages: 25-31
  • URL: http://www.js-phlebology.org/journal/index
  • Peer Reviewed
• [Journal Article] バージャー病患者の適遥性静脈炎、表在静脈弁不全についての考察と新知見 (2011)
  • Author(s): 岩井武尚, 佐藤彰治, 久米博子, 井上芳徳, 梅田誠, 加賀山知子, 広川雅之
  • Journal Title: 静脈学 Volume: 22 Pages: 25-31
  • Peer Reviewed
• [Journal Article] Association between periodontitis and anti-cardiolipin antibodies in Buerger disease (2009)
  • Author(s): Chen YW, Nagasawa T, Wara-Aswapati N, Ushida Y, Wang D, Takeuchi Y, Kobayashi H, Umeda M, Inoue Y, Iwai T, Ishikawa I, Izumi Y
  • Journal Title: J Clin Periodontol Volume: 36巻 Pages: 830-835
  • Peer Reviewed
• [Journal Article] Periodontitis and cardiovascular diseases : The link and relevant mechanisms (2009)
  • Author(s): Izumi Y, Nagasawa T, Umeda M, Kobayashi H, Takeuchi Y, Yashiro R, Hormdee D, Suda T, Ushida Y, WaraAswapati N
  • Journal Title: Japanese Dental Science Review Volume: 45巻 Issue: 2 Pages: 98-108
  • DOI: 10.1016/j.jdsr.2009.06.001
  • NAID: 10025820929
  • Peer Reviewed
• [Journal Article] Association between periodontitis and anti-cardiolipin antibodies in Buerger disease (2009)
  • Author(s): Chen YW, Nagasawa T, Wara-Aswapati, et.al.
  • Journal Title: Journal of Clinical Periodontology 36 Pages: 830-835
  • Peer Reviewed
• [Journal Article] Periodontitis and cardiovascular diseases : The link and relevant mechanisms (2009)
  • Author(s): IzumiY, Nagasawa T, Umeda M, et.al.
  • Journal Title: Japanese Dental Science Review 45 Pages: 98-108
  • NAID: 10025820929
  • Peer Reviewed
• [Presentation] 新規に開発したモノクローナル抗体を用いた歯肉組織中の歯周病原細菌の局在の解明 (2011)
  • Author(s): RaJakaruna GA, 梅田誠、内田佳介、古川あすか、和泉雄一、江石義信
  • Organizer: 第59回国際歯科研究学会日本部会(JADR)
  • Place of Presentation: 広島
  • Year and Date: 2011-10-08
• [Presentation] 新規に開発したモノクローナル抗体を用いた病的歯肉組織中の歯周病原細菌の検出と局在 (2011)
  • Author(s): RaJakaruna GA, 内田佳介、梅田誠、古川あすか、和泉雄一、江石義信
  • Organizer: 第54回秋季日本歯周病学会学術大会
  • Place of Presentation: 下関
  • Year and Date: 2011-09-24
• [Presentation] Detection of periodontal pathogens in gingival tissues using novel MAb (2010)
  • Author(s): Rajakaruna GA, Uchida K, Umeda M, Furukawa A, Suzuki Y, Lokman N, Izumi Y, Eishi Y
  • Organizer: 第58回国際歯科研究学会日本部会(JADR)総会・学術大会
  • Place of Presentation: 北九州市
  • Year and Date: 2010-11-20
• [Presentation] Detection of Periodontal Pathogens in Gingival Tissues using Novel MAb (2010)
  • Author(s): Rajakaruna GA, Uchida K, Umeda M, et.al.
  • Organizer: 第58回国際歯科研究学会日本部会(JADR)総会・学術大会
  • Place of Presentation: 北九州市
  • Year and Date: 2010-11-20
• [Book] ザ・ペリオドントロジー (2009)
  • Author(s): 梅田誠、和泉雄一
  • Publisher: 永末書店
• [Book] ザ・ペリオドントロジー (2009)
  • Author(s): 和泉雄一、沼部幸博、山本松男、木下淳博 編
  • Total Pages: 294
  • Publisher: 永末書店