Study on novel tissue regenerative therapy using bone marrow-derived mesenchymal stem cells and FGF/Phosphophoryn/Collagen complex

• Project/Area Number: 21592630
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Matsumoto Dental University (2011); Health Sciences University of Hokkaido (2009-2010)
• Principal Investigator: FUJII Takeo 松本歯科大学, 歯学部・附属病院, 臨床教授 (30173389)
• Co-Investigator(Kenkyū-buntansha): SAITO Takashi 北海道医療大学, 歯学部, 教授 (40265070) ; ABIKO Yoshihiro 北海道医療大学, 歯学部, 教授 (90260819) ; SAITO Masato 北海道医療大学, 歯学部, 教授 (50337036)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 歯周外科学 / 再生治療 / 三次元培養系 / 歯髄間葉系幹細胞 / ホスホホリン / FGF / 再生医療 / 歯周組織再生 / 骨髄間葉系幹細胞

Research Abstract

With the goal to establish new tissue regenerative therapy for extensive regeneration of destroyed periodontal tissues accompanying vertical or horizontal bone defects due to severe periodontitis, The aim of this study was to evaluate the kinetics of bone marrow-derived mesenchymal stem cells cultured in a FGF. collagen complex as a regenerative scaffold in vitro. Bone marrow-derived mesenchymal stem cells showed good growth in the collagen gel culture. The effect of FGF in promoting cell growth is exhibited at the early stage of culture, and the ALP activity has already passed the peak when assayed at 14 days of culture at FGF concentrations of 10 ng/ml. The ALP activity showed in the presence of phosphophoryn in a dose-dependent manner at 14 days of culture. A marked difference in calcium content was not observed. The present study sheds light on the development of an optimal biomaterial that forms the basis of calcified hard tissue formation, suggesting the feasibility of applying the 3-D collagen gel cell culture system for the construction of a scaffold that controls the amount of regenerating tissue.

Research Products

• [Journal Article] 歯周炎罹患歯に対するFGF-2投与の長期的効果および安全性の検討 (2012)
  • Author(s): 北村正博、古市保志、藤井健男、川浪雅光、国松和司、島内英俊、山田了、小方頼昌、和泉雄一、伊藤公一、中川種昭、新井高、山崎和久、吉江弘正、野口俊英、渋谷俊昭、高柴正悟、栗原英見、永田俊彦、横田誠、前田勝正、廣藤卓雄、坂上竜資、原宣興、野口和行、小笠原健文、村上伸也
  • Journal Title: 日歯周誌 Volume: 54 Pages: 38-45
  • NAID: 10030555232
  • Peer Reviewed
• [Journal Article] Murakami FGF-2 stimulates periodontal regeneration : results of multicenter randomized clinical trial (2011)
  • Author(s): Kitamura M, Akamatsu M, Machigashira M, Hara Y, Sakagami R, Hirofuji T, Hamachi T, Maeda K, Yokota M, Kido J, Nagata T, Kurihara H, Takashiba S, Sibutani T, Fukuda M, Noguchi T, Yamazaki K, Yoshie H, Ioroi K, Arai T, Nakagawa T, Ito K, Oda S, Izumi Y, Ogata Y, Yamada S, Shimauchi H, Kunimatsu K, K awanami M, Fujii T, Furuichi Y, Furuuchi T, Sasano T, Imai E, Omae M, Yamada S, Watanuki M
  • Journal Title: J Dent Res Volume: 90(1) Pages: 35-40
  • Peer Reviewed
• [Journal Article] FGF-2 stimulates periodontal regeneration : results of a multi-center randomized clinical trial (2011)
  • Author(s): Kitamura M, Akamatsu M, Machigashira M, 他35名
  • Journal Title: J Dent Res Volume: 90 Issue: 1 Pages: 35-40
  • DOI: 10.1177/0022034510384616
  • Peer Reviewed
• [Journal Article] FGF-2 stimulates periodontal regeneration : results of multicenter randomized clinical trial. (2011)
  • Author(s): Kitamura M, Akamatsu M,Fujii, T, et al.
  • Journal Title: J Dent Res. Volume: 90(1) Pages: 35-40
  • Peer Reviewed
• [Journal Article] Periodontal Tissue Regeneration by the Application of FGF-2 in Periodontitis Patients. (2009)
  • Author(s): T.Fujii, Y.Terada, M.Akamatsu, M.Watanuki.
  • Journal Title: J Clin Periodontol 36 Pages: 212-217
  • Peer Reviewed
• [Presentation] Study on novel tissue regenerative therapy using bone marrow-derived mesenchymal stem cells and FGF/collagen complex (2011)
  • Author(s): T. Fujii, T. Saito, A. Yamaguchi, K. Shimizu, T. Taira
  • Organizer: The 3^<rd> Joint Meeting of ECTS & IBMS
  • Place of Presentation: Athens, Greece
  • Year and Date: 2011-05-07
• [Presentation] Periodontal Tissue Regeneration by the Application of FGF-2 in Patients (2010)
  • Author(s): T. Fujii, Y. Terada, M. Akamatsu, M. Watanuki
  • Organizer: 37^<th> European Symposium on Calcified Tissues
  • Place of Presentation: Glasgow, Scotland
  • Year and Date: 2010-06-21
• [Presentation] Periodontal Tissue Regeneration by the Application of FGF-2 in Periodontitis Patients (2010)
  • Author(s): T.Fujii, Y.Terada, M.Akamatsu, M.Watanuki
  • Organizer: 37^<th> European Symposium on Calcified Tissues
  • Place of Presentation: Glasgow, Scotland
  • Year and Date: 2010-06-21
• [Presentation] Periodontal Tissue Regeneration by the Application of FGF-2 in Periodontitis Patients (2009)
  • Author(s): T. Fujii, Y. Terada, Y. Konishi, H. Tsuji, J. Sato
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 2009-06-04
• [Presentation] Periodontal Tissue Regeneration by the Application of FGF-2 in Periodontitis Patients. (2009)
  • Author(s): T.Fujii, Y.Terada, Y.Konishi, H.Tsuji, J.Sato, et al.
  • Organizer: Europerio 6
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 2009-06-04