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Establishment of an effective three dimensional culture model for screening of molecular targeted drug for periodontitis

Research Project

Project/Area Number 21592637
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Periodontal dentistry
Research InstitutionOhu University (2010-2012)
Nihon University (2009)

Principal Investigator

OHSHIMA Mitsuhiro  奥羽大学, 薬学部, 教授 (30194145)

Co-Investigator(Kenkyū-buntansha) YAMAGUCHI Yoko  日本大学, 歯学部, 助手 (00239922)
Co-Investigator(Renkei-kenkyūsha) ABIKO Yoshimitsu (KATO Mitsuyasu)  日本大学, 松戸歯学部, 教授 (70050086)
Project Period (FY) 2009 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords歯周免疫機能学 / 生体外歯周炎モデル / 三次元培養 / コラーゲン分解阻害 / 歯周炎原因遺伝子 / 分子標的治療薬 / コラーゲン分解阻害剤 / 歯周炎 / コラーゲン分解
Research Abstract

“In vitro periodontitis model” using collagen gel three-dimensional culture was established combining primary cultured gingival epithelial cells and gingival fibroblasts. Transcriptome analyses revealed several candidate genes for periodontitis. Furthermore, inhibition of activity of FLT-1, one of the products of candidate genes for periodontitis, using small molecule kinase inhibitor successfully controlled collagen gel degradation, suggested a possibility to use this kinase inhibitor as a molecular-targeted drug for periodontitis.

Report

(5 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (47 results)

All 2013 2012 2011 2010 2009 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (21 results) Remarks (2 results) Patent(Industrial Property Rights) (14 results)

  • [Journal Article] 歯周炎薬物治療のパラダイムシフト(総説)2013

    • Author(s)
      大島光宏、山口洋子
    • Journal Title

      日薬理誌

      Volume: 141 Pages: 314-320

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] High-frequency low-level diode laser irradiation promotes proliferation and migration of primary cultured human gingival epithelial cells2013

    • Author(s)
      Kenichiro Ejiri, Akira Aoki, Yoko Yamaguchi, Mitsuhiro Ohshima, Yuichi Izumi
    • Journal Title

      Lasers Med Sci 2013

      Volume: (印刷中)

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] An integrated expression profiling reveals target genes of TGF-β and TNF-α possibly mediated by microRNAs in lung cancer cells2013

    • Author(s)
      Akira Saito, Hiroshi I Iizuka, Masafumi Horie, Mitsuhiro Ohshima, Yasuyuki Morishita, Yoshimitsu Abiko, Takahide Nagase
    • Journal Title

      PLoS ONE 8

      Volume: 8 Issue: 1 Pages: 158-163

    • DOI

      10.1016/j.bbrc.2012.05.104

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 歯周炎薬物治療のパラダイムシフト(総説)2013

    • Author(s)
      大島光宏
    • Journal Title

      日本薬理学雑誌

      Volume: 141

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High-frequency low-level diode laser irradiation promotes proliferation and migration of primary cultured human gingival epithelial cells2013

    • Author(s)
      Kenichiro Ejiri
    • Journal Title

      Lasers Med Sci

      Volume: 未定 Issue: 4 Pages: 1339-1347

    • DOI

      10.1007/s10103-013-1292-7

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of human lung cancer-associated fibroblasts in three dimensional in vitro co-culture model2012

    • Author(s)
      Masafumi Horie, Akira Saito, Mitsuhiro Ohshima, Yasuyuki Morishita, Jun Nakajima, Tadashi Kohyama, Takahide Nagase
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 423 Issue: 2 Pages: 158-163

    • DOI

      10.1371/journal.pone.0056587

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Insulin-like growth factor-binding protein-2 and -3 in gingival crevicular fluid2010

    • Author(s)
      Takenouchi Y, Ohshima M, Yamaguchi Y, Nishida T, Senda N, Idesawa M, Otsuka K, Ito K
    • Journal Title

      J Periodontal Res

      Volume: 45 Issue: 6 Pages: 803-808

    • DOI

      10.1111/j.1600-0765.2010.01309.x

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] TGF-b signaling in gingival fibroblast-epithelial interaction2010

    • Author(s)
      M. Ohshima, Y. Yamaguchi, M. Matsumoto, P. Micke, T. Takenouchi, N. Nishida, M. Kato, K. Komiyama, Y. Abiko, K. Ito, K. Otsuka, K. Kappert
    • Journal Title

      J Dent Res

      Volume: 89 Issue: 11 Pages: 1315-1321

    • DOI

      10.1177/0022034510378423

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] TGF-β signaling in gingival-epithelial interaction2010

    • Author(s)
      M.Ohshima, Y.Yamaguchi, ほか
    • Journal Title

      Journal of Dental Research

      Volume: 89 Pages: 1315-1321

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Insulin-like growth factor-binding protein-2 and -3 in gingival crevicular fluid2010

    • Author(s)
      Y.Takenouchi, M.Ohshima, ほか
    • Journal Title

      Journal of Periodontal Research

      Volume: 45 Pages: 803-808

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Super-pulsed low-level diode Laser stimulates cultured human gingival epithelial cells2013

    • Author(s)
      Kenichiro Ejiri, Akira Aoki, Yoko Yamaguchi,Mitsuhiro Ohshima, Yuichi Izumi
    • Organizer
      91^<st>IADR
    • Place of Presentation
      Seattle, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] Super-pulsed Low-level Diode Laser Stimulates Cultured Human Gingival Epithelial Cells2013

    • Author(s)
      Kenichiro Ejiri
    • Organizer
      91st General Session & Exhibition of the IADR
    • Place of Presentation
      Seattle, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] 低出力半導体レーザー照射が初代培養ヒト歯肉上皮細胞に及ぼす効果2012

    • Author(s)
      江尻健一郎、青木 章、山口洋子、大島光宏、和泉雄一
    • Organizer
      第24回日本レーザー歯学会総会・学術大会
    • Place of Presentation
      神戸
    • Related Report
      2012 Final Research Report
  • [Presentation] FLT-1は歯周炎治療の標的分子となり得るか2012

    • Author(s)
      大島光宏、山口洋子、安孫子宜光
    • Organizer
      第54回歯科基礎医学会学術大会・総会
    • Place of Presentation
      郡山
    • Related Report
      2012 Final Research Report
  • [Presentation]2012

    • Author(s)
      大島光宏、山口洋子、張 月琳、佐久間淳
    • Organizer
      2012年度日本機会学会年次大会
    • Place of Presentation
      金沢
    • Related Report
      2012 Final Research Report
  • [Presentation] 低出力半導体レーザー照射が培養ヒト歯肉上皮細胞に及ぼす効果2012

    • Author(s)
      江尻健一郎、青木 章、山口洋子、大島光宏、和泉雄一
    • Organizer
      第136回日本歯科保存学会春季学術大会
    • Place of Presentation
      沖縄
    • Related Report
      2012 Final Research Report
  • [Presentation] FLT-1 represents a potential therapiutic target in human periodontitis2011

    • Author(s)
      Ohshima M, Yamaguchi Y, Abiko Y
    • Organizer
      Gordon Research Conference in Periodontal Diseases
    • Place of Presentation
      Davidson College, Davidson, NC
    • Year and Date
      2011-07-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] 受容体チロシンキナーゼ阻害剤による非外科的顎〓胞治療法の開発2011

    • Author(s)
      大島光宏、山口洋子、石井輝彦、大木秀郎
    • Organizer
      第83回日本生化学会大会
    • Place of Presentation
      京都
    • Related Report
      2012 Final Research Report
  • [Presentation] FLT-1 represents a potential therapeutic target in human periodontitis2011

    • Author(s)
      Mitsuhiro Ohshima, Yoko Yamaguchi, Yoshimitsu Abiko
    • Organizer
      GRC in Periodontal Diseases
    • Place of Presentation
      Davidson College, Davidson, NC, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] Screening of finished herbal products using in vitro periodontitis model2010

    • Author(s)
      Y.Yamaguchi, K.Toriizuka, M.Ohshima
    • Organizer
      BMB2010(第33回日本分子生物学会年会、第83回日本生化学会大会合同大会)
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] 生薬による生体外歯周炎モデルのコラーゲン分解阻害2010

    • Author(s)
      山口洋子, 鳥居塚和生, 大島光宏
    • Organizer
      第49回日本薬学会東北支部大会
    • Place of Presentation
      奥羽大学(郡山)
    • Year and Date
      2010-10-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] 三次元培養法を用いた新規歯周炎治療薬のスクリーニング2010

    • Author(s)
      山口洋子, 大島光宏
    • Organizer
      第42回日本結合組織学会、第57回マトリックス研究会合同学術集会
    • Place of Presentation
      秋田拠点センター・アルヴェ
    • Year and Date
      2010-08-19
    • Related Report
      2010 Annual Research Report
  • [Presentation] 生体外歯周炎モデルによる生薬のスクリーニン グ2010

    • Author(s)
      山口洋子、鳥居塚和生、大島光宏
    • Organizer
      第33回日本分子生物学会年会、第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Related Report
      2012 Final Research Report
  • [Presentation] 生薬による生体外歯周炎モデルのコラーゲン分解阻害2010

    • Author(s)
      山口洋子、鳥居塚和生、大島光宏
    • Organizer
      第49回日本薬学会東北支部大会
    • Place of Presentation
      郡山
    • Related Report
      2012 Final Research Report
  • [Presentation] 三次元培養法を用いた新規歯周炎治療薬のスクリーニング2010

    • Author(s)
      山口洋子、大島光宏
    • Organizer
      第42回日本結合組織学会・第57回マトリックス研究会合同学術集会
    • Place of Presentation
      秋田
    • Related Report
      2012 Final Research Report
  • [Presentation] A novel drug screening system for periodontitis using 3D culture2010

    • Author(s)
      Ohshima M, Yamaguchi Y, Abiko Y
    • Organizer
      GRC in Periodontal Diseases
    • Place of Presentation
      Colby-Sawyer College, New London, NH, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] A novel drug screening system for periodontitis using 3D culture2009

    • Author(s)
      Ohshima M
    • Organizer
      歯周病ゴードン会議
    • Place of Presentation
      Colby-Sawyer College, New London, NH,(USA)
    • Year and Date
      2009-08-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] 低出力半導体レーザー照射が初代培養ヒト歯肉上皮細胞に及ぼす影響

    • Author(s)
      江尻健一郎
    • Organizer
      第24回日本レーザー歯学会総会学術大会
    • Place of Presentation
      神戸
    • Related Report
      2012 Annual Research Report
  • [Presentation] FLT-1は歯周炎治療の標的分子となり得るか

    • Author(s)
      大島光宏
    • Organizer
      第54回歯科基礎医学会学術大会・総会
    • Place of Presentation
      郡山
    • Related Report
      2012 Annual Research Report
  • [Presentation] 歯周炎治療薬候補のスクリーニングにおける押し込み試験の有用性

    • Author(s)
      大島光宏
    • Organizer
      2012年度日本機械学会年次大会
    • Place of Presentation
      金沢
    • Related Report
      2012 Annual Research Report
  • [Presentation] 低出力半導体レーザー照射が培養ヒト歯肉上皮細胞に及ぼす効果

    • Author(s)
      江尻健一郎
    • Organizer
      日本歯科保存学会2012年度春季学術大会
    • Place of Presentation
      沖縄
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.ohu-u.ac.jp/newinfo/2010/20100924.html

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.ohu-u.ac.jp/newinfo/2010/20100924.html

    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 歯周炎治療薬2012

    • Inventor(s)
      山口洋子、大島光宏
    • Industrial Property Rights Holder
      日本大学
    • Industrial Property Number
      2012-086327
    • Filing Date
      2012-04-05
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 歯周炎治療薬2012

    • Inventor(s)
      山口洋子,大島光宏
    • Industrial Property Rights Holder
      日本大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2012-086327
    • Filing Date
      2012-04-05
    • Related Report
      2012 Annual Research Report
  • [Patent(Industrial Property Rights)] 歯周炎治療薬2012

    • Inventor(s)
      大島光宏、山口洋子
    • Industrial Property Rights Holder
      日本大学
    • Industrial Property Number
      2012-086327
    • Filing Date
      2012-04-05
    • Related Report
      2011 Annual Research Report
  • [Patent(Industrial Property Rights)] 口腔癌細胞浸潤阻害剤2011

    • Inventor(s)
      山口洋子、大島光宏、鳥居塚和生
    • Industrial Property Rights Holder
      日本大学、昭和大学
    • Industrial Property Number
      2011-220423
    • Filing Date
      2011-10-04
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 口腔癌細胞浸潤阻害剤2011

    • Inventor(s)
      大島光宏、山口洋子、鳥居塚和生
    • Industrial Property Rights Holder
      日本大学
    • Industrial Property Number
      2011-220423
    • Filing Date
      2011-10-04
    • Related Report
      2011 Annual Research Report
  • [Patent(Industrial Property Rights)] 顎嚢胞治療剤2010

    • Inventor(s)
      山口洋子、大島光宏
    • Industrial Property Rights Holder
      日本大学
    • Industrial Property Number
      2010-152872
    • Filing Date
      2010-07-05
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] コラーゲン分解阻害剤2010

    • Inventor(s)
      大島光宏、山口洋子、鳥居塚和生
    • Industrial Property Rights Holder
      日本大学、昭和大学
    • Industrial Property Number
      2010-132206
    • Filing Date
      2010-06-09
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 歯周炎治療薬2010

    • Inventor(s)
      大島光宏、山口洋子
    • Industrial Property Rights Holder
      日本大学
    • Industrial Property Number
      2010-024339
    • Filing Date
      2010-02-05
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] コラーゲン分解阻害剤2010

    • Inventor(s)
      大島光宏, 山口洋子, 鳥居塚和生
    • Industrial Property Rights Holder
      日本大学昭和大学
    • Industrial Property Number
      2010-132206
    • Filing Date
      2010-06-09
    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 歯周炎治療薬2010

    • Inventor(s)
      大島光宏, 山口洋子
    • Industrial Property Rights Holder
      日本大学
    • Industrial Property Number
      2010-024339
    • Filing Date
      2010-02-05
    • Related Report
      2009 Annual Research Report
  • [Patent(Industrial Property Rights)] 歯肉上皮細胞の培養方法2009

    • Inventor(s)
      山口洋子、大島光宏
    • Industrial Property Rights Holder
      日本大学
    • Industrial Property Number
      2009-231339
    • Filing Date
      2009-10-05
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 生体外創傷治癒試験用用具または創傷作製方法2009

    • Inventor(s)
      山口洋子、大島光宏
    • Industrial Property Rights Holder
      日本大学
    • Industrial Property Number
      2009-212903
    • Filing Date
      2009-09-15
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 生体外創傷治癒試験用用具または創傷作製方法2009

    • Inventor(s)
      山口洋子, 大島光宏
    • Industrial Property Rights Holder
      日本大学
    • Industrial Property Number
      2009-212903
    • Filing Date
      2009-09-15
    • Related Report
      2009 Annual Research Report
  • [Patent(Industrial Property Rights)] 歯肉上皮細胞の培養方法2009

    • Inventor(s)
      山口洋子, 大島光宏
    • Industrial Property Rights Holder
      日本大学
    • Industrial Property Number
      2009-231339
    • Filing Date
      2009-10-05
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2019-07-29  

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