Development of a method for analysis of oral microbiota applicable to a large-scale population
| Project/Area Number |
21592652
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Social dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 予防歯科学 / 細菌叢 / 16S rRNA / T-RFLP |
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| Research Abstract |
There are several hundreds species of oral bacteria in human oral cavities. We constructed a system for effective calculation using Terminal Restriction Fragment Length Polymorphism, T-RFLP, in order to calculate compositions of oral microbial species. We succeeded in estimating compositions of bacterial species by a Monte Carlo algorithm. This program estimates proportional compositions by minimizing distances between peak values and the relative abundance of each group, containing several species, estimated from peak areas of capil-lary electrophoresis for T-RFLP analysis. In addition, databases of 16S rRNA sequences from oral and intestine microbiota to calculate bacterial composition by T-RFLP analyses.
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Report
(4 results)
Research Products
(17 results)
