Mechanisms of drug efflux by two-component control system in oral Streptococci

• Project/Area Number: 21592653
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Social dentistry
• Research Institution: Kyushu University
• Principal Investigator: SHIOTA Susumu 九州大学, 歯学研究院, 助教 (00150467)
• Co-Investigator(Renkei-kenkyūsha): YAMASHITA Yoshihisa (20192403)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 細菌 / シグナル伝達 / 発現制御

Research Abstract

Streptococcus mutans, a major etiological agent of dental caries, is resistant to bacitracin. We studied the mechanisms of two-component signal transduction system(TCS) that controlled the expression of the bacitracin resistant operon. As a result, under the bacitracin stimulation the response regulator of TCS specifically bind to a putative promoter region of ABC transpoter genes, then accelerate the expression of these genes. The mutant that substituted asparagine for aspartic acid at position 54 could not bind to a putative promoter and also shows the susceptibility to bacitracin.

Research Products

• [Journal Article] Characterization of MbrCinvolved in bacitracin resistance in Streptococcus mutans (2011)
  • Author(s): Norio Kitagawa, Susumu Shiota, Yukie Shibata, Toru Takeshitaand Yoshihisa Yamashita
  • Journal Title: FEMS Microbiol Lett Volume: 388 Pages: 61-67
  • Peer Reviewed
• [Journal Article] Characterizaition of MbrC involved in bacitracin resistance in Streptococcus mutans (2011)
  • Author(s): Norio Kitagawa, Susumu Shiota, Yukie Shibata, Toru Takeshita, Yoshihisa Yamashita
  • Journal Title: FEMS Microbiology Letters Volume: 318 Pages: 61-67
  • Peer Reviewed
• [Presentation] MbrCの5 4番目のアスパラギン酸がStreptococcus mutansのバシトラシン耐性に関与する (2010)
  • Author(s): 北河憲夫
  • Organizer: 歯科基礎医学会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-21
• [Presentation] MbrCの54番目のアスパラギン酸がStreptococcus mutansのバシトラシン耐性に関与する (2010)
  • Author(s): 北河憲雄、塩田進、柴田幸江、竹下徹、山下善久
  • Organizer: 第52回歯科基礎医学会学術大会・総会
  • Place of Presentation: タワーホール船堀(東京)
  • Year and Date: 2010-09-21
• [Presentation] Streptococcus mutansの二成分制御系によるバシトラシン耐性機構 (2009)
  • Author(s): 北河憲夫
  • Organizer: 歯科基礎医学会
  • Place of Presentation: 新潟
  • Year and Date: 2009-09-10
• [Presentation] Streptococcus mutans の二成分制御系によるバシトラシン耐性機構 (2009)
  • Author(s): 北河憲雄
  • Organizer: 歯科基礎医学会
  • Place of Presentation: 新潟市
  • Year and Date: 2009-09-10