Construction of mutanase chimer ic enzyme by domain shuffling and degradation of biofilm by the chimeric enzyme
| Project/Area Number |
21592669
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Social dentistry
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| Research Institution | Tsurumi University |
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Principal Investigator |
IMAI Susumu 鶴見大学, 歯学部, 講師 (80072958)
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| Co-Investigator(Kenkyū-buntansha) |
HANADA Nobuhiro 鶴見大学, 歯学部, 教授 (70180916)
NOMURA Yoshiaki 鶴見大学, 歯学部, 准教授 (90350587)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | バイオフィルム / ムタナーゼ / デキストラナーゼ / キメラ酵素 / ドメインシャッフリング |
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| Research Abstract |
Cariogenic mutans streptococci produce water-insoluble glucan involving in biofilm formation from sucrose as a substrate. The purpose of this study was to construct chimeric enzyme composed of dextranase and mutanase that degrade α-1, 6- and α-1, 3-glucosidic bond, respectively. Dextranase gene and mutanase gene were cloned in plasmid and the chimeric gene was successfully constructed in the expression vector and the resulting enzyme hydrolyzed effectively dextran and mutan simultaneously. It is desired to improve the chimeric enzyme to highly functional one in the future.
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Report
(4 results)
Research Products
(37 results)
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[Presentation] Oral status of chimpanzees in primate research institute Kyoto University.2010
Author(s)
Momoi Y., Saito W., Ogawa T., Ikawa T., Nomura Y., Imai S., Hanada N., Miyabe, Y., et al
Organizer
Japanese Association for Dental research 58^<th> annual meeting
Place of Presentation
Kitakyushu, Japan
Year and Date
2010-11-20
Related Report
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[Presentation] Oral status of chimp anzees in primate research institute Kyoto University2010
Author(s)
Momoi Y., Saito W., Ogawa T., Ikaw a T., Nomura Y., Imai S., Hanada N., Miyabe, Y., et al
Organizer
Japanese Association for Dental research, 58^<th> annual meeting
Place of Presentation
Kitakyushu, Japan
Related Report
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