Establishment of artificial synthesis of Nano bacteria and dental calculus

• Project/Area Number: 21592670
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Social dentistry
• Research Institution: Tsurumi University
• Principal Investigator: NOMURA Yoshiaki 鶴見大学, 歯学部, 准教授 (90350587)
• Co-Investigator(Kenkyū-buntansha): HANADA Nobuhiro 鶴見大学, 歯学部, 教授 (70180916)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 石灰化 / ナノ粒子 / 歯学 / 社会医学

Research Abstract

We tried to purify the proteins that react to the monoclonal antibody for the Nano bacteria from the bovine serum. These proteins strongly bind to the hydroxyapatite. Among these proteins, one of them binds to hydroxyapatite by cationic reactions and others are bind to hydroxyapatite by affinity reactions for the calcium. We success in the purification of the protein that bind to hydroxyapatite by cationic reactions. By the gel filtration chromatography, other proteins that bind to the hydroxyapatite by affinity reactions for the calcium were collected in fixed fractions, however, we could not single out these proteins. By the mass analysis, these proteins included the components of the common fatty acid complexes that related to the life style diseases.

Research Products

• [Journal Article] Streptococcus troglodytae sp. nov., from the Chimpanzee Oral Cavity (2012)
  • Author(s): Okamoto M, Imai S, Miyanohara M, Saito W, Momoi Y, Abo T, Nomura Y, Ikawa T, Ogawa T, Miyabe-Nishiwaki T, Kaneko A, Watanabe A, Watanabe S, Hayashi M, Tomonaga M, Hanada N
  • Journal Title: Int J Syst Evol Microbiol
• [Journal Article] Human periodontal ligament fibroblasts are the optimal cell source for induced pluripotent stem cells (2012)
  • Author(s): Nomura Y, Ishikawa M, Yashiro Y, Sanggarnjanavanich S, Yamaguchi T, Arai C, Noda K, Takano Y, Nakamura Y, Hanada N
  • Journal Title: Histochem Cell Biol
• [Journal Article] Salivary biomarkers for predicting the progression of chronic periodontitis (2012)
  • Author(s): Nomura Y, Shimada Y, Hanada N, Numabe Y, Kamoi K, Sato T, Gomi K, Arai T, Inagaki K, Fukuda M, Noguchi T, Yoshie H
  • Journal Title: Arch Oral Biol Volume: 57(4) Pages: 413-20
• [Journal Article] Streptococcus troglodytae sp.nov., from the Chimpanzee Oral Cavity (2012)
  • Author(s): Okamoto M, Imai S, Miyanohara M, Saito W, Momoi Y, Abo T, Nomura Y, Ikawa T, Ogawa T, Miyabe-Nishiwaki T, Kaneko A, Watanabe A, Watanabe S, Hayashi M, Tomonaga M, Hanada N
  • Journal Title: Int J Syst Evol Microbiol
  • Peer Reviewed
• [Journal Article] Human periodontal ligament fibroblasts are the optimal cell source for induced pluripotent stem cells (2012)
  • Author(s): Nomura Y, Ishikawa M, Yashiro Y, Sanggarnjanavanich S, Yamaguchi T, Arai C, Noda K, Takano Y, Nakamura Y, Hanada N
  • Journal Title: Histochem Cell Biol.2012
  • Peer Reviewed
• [Journal Article] Mechanism of active eruption of molars in adolescent rats (2011)
  • Author(s): Oikawa T, Nomura Y, Arai C, Noda K, Hanada N, Nakamura Y
  • Journal Title: Eur J Orthod Volume: 33(3) Pages: 221-7
• [Journal Article] HSPA1A is upregulated in periodontal ligament at early stage of tooth movement in rats (2010)
  • Author(s): Arai C, Nomura Y, Ishikawa M, Noda K, Choi JW, Yashiro Y, Hanada N, Nakamura Y
  • Journal Title: Histochem Cell Biol Volume: 134(4) Pages: 337-43
• [Journal Article] HSPA1A is upregulated in periodontal ligament at early stage of tooth movement in rats. (2010)
  • Author(s): Arai C, Nomura Y, Ishikawa M, Noda K, Choi JW, Yashiro Y, Hanada N, Nakamura Y.
  • Journal Title: Histochem Cell Biol. Volume: 134(4) Pages: 337-343
  • Peer Reviewed
• [Journal Article] 歯周病患者の特に喫煙と糖尿病に関連した健康意識調査と健康状態に関するアンケート調査 (2010)
  • Author(s): 長岐祐子, 漆崎絵美, 高野聡美, 三辺正人, 漆原譲治, 野村義明
  • Journal Title: 日本歯周病学会会誌 Volume: 52巻1号 Pages: 73-82
  • NAID: 10027096206
  • Peer Reviewed
• [Journal Article] Asymmetry of the lips of orthognathic surgery patients. (2009)
  • Author(s): Yamashita Y, Nakamura Y, Shimada T, Nomura Y, Hirashita A.
  • Journal Title: Am J Orthod Dentofacial Orthop 136(4) Pages: 559-563
  • Peer Reviewed
• [Journal Article] Morphological changes in the rat periodontal ligament and its vascularity after experimental tooth movement using superelastic forces. (2009)
  • Author(s): Noda K, Nakamura Y, Kogure K, Nomura Y.
  • Journal Title: Eur J Orthod. 31(1) Pages: 37-45
  • Peer Reviewed
• [Presentation] iPS細胞の安全性に関する検討第2報核型異常について (2012)
  • Author(s): 野村義明, 石川美佐緒, 新井千博, 八城祐一, 野田晃司, 山口貴央, 阿保備子, 角田衣理加, 山田秀則, 今井奨, 中村芳樹, 花田信弘
  • Organizer: 第61回日本口腔衛生学会総会
  • Place of Presentation: 横須賀市
• [Presentation] iPS細胞の安全性に関する検討 (2011)
  • Author(s): 野村義明, 石川美佐緒, 新井千博, 八城祐一, 野田晃司, 山口貴央, 阿保備子, 角田衣理加, 山田秀則, 今井奨, 中村芳樹, 花田信弘
  • Organizer: 第60回日本口腔衛生学会総会
  • Place of Presentation: 松戸市
• [Presentation] チンパンジー口腔由来のレンサ球菌の性状に関する研究 (2011)
  • Author(s): 宮之原真由, 今井奨, 齋藤渉, 山口貴央, 阿保備子, 野村義明, 桃井保子, 花田信弘
  • Organizer: 第60回日本口腔衛生学会総会
  • Place of Presentation: 松戸市
• [Presentation] 唾液検査を用いた歯周病検診受診者の意識と行動の調査 (2011)
  • Author(s): 花田信弘, 矢吹義秀, 小林憲司, 古藤真実, 福澤洋一, 谷村秀樹, 中曽根隆一, 野村義明
  • Organizer: 第60回日本口腔衛生学会総会
  • Place of Presentation: 松戸市
• [Presentation] ヒト歯根膜線維芽細胞からのiPS細胞の樹立 (2011)
  • Author(s): 石川美佐緒, 野村義明, 八城祐一, 新井千博, 山口貴央, 村田貴俊, 野田晃司, 石川雄一, 花田信弘, 中村芳樹
  • Organizer: 第53回歯科基礎医学会総会
  • Place of Presentation: 岐阜市
• [Presentation] ムタナーゼとデキストラナーゼ両遺伝子の連結によるバイオフィルムの分解 (2011)
  • Author(s): 角田衣理加, 野村義明, 村田貴俊, 今井奨, 津守秀明, 花田信弘
  • Organizer: 第53回歯科基礎医学会総会
  • Place of Presentation: 岐阜市
• [Presentation] 細胞への硫化水素暴露により発現が上昇するPHLDA1 (2011)
  • Author(s): 村田貴俊, 角田衣理加, 野村義明, 今井奨, 花田信弘
  • Organizer: 第53回歯科基礎医学会総会
  • Place of Presentation: 岐阜市