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Establishment of artificial synthesis of Nano bacteria and dental calculus

Research Project

Project/Area Number 21592670
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Social dentistry
Research InstitutionTsurumi University

Principal Investigator

NOMURA Yoshiaki  鶴見大学, 歯学部, 准教授 (90350587)

Co-Investigator(Kenkyū-buntansha) HANADA Nobuhiro  鶴見大学, 歯学部, 教授 (70180916)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords石灰化 / ナノ粒子 / 歯学 / 社会医学
Research Abstract

We tried to purify the proteins that react to the monoclonal antibody for the Nano bacteria from the bovine serum. These proteins strongly bind to the hydroxyapatite. Among these proteins, one of them binds to hydroxyapatite by cationic reactions and others are bind to hydroxyapatite by affinity reactions for the calcium. We success in the purification of the protein that bind to hydroxyapatite by cationic reactions. By the gel filtration chromatography, other proteins that bind to the hydroxyapatite by affinity reactions for the calcium were collected in fixed fractions, however, we could not single out these proteins. By the mass analysis, these proteins included the components of the common fatty acid complexes that related to the life style diseases.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (18 results)

All 2012 2011 2010 2009

All Journal Article (11 results) (of which Peer Reviewed: 6 results) Presentation (7 results)

  • [Journal Article] Streptococcus troglodytae sp. nov., from the Chimpanzee Oral Cavity2012

    • Author(s)
      Okamoto M, Imai S, Miyanohara M, Saito W, Momoi Y, Abo T, Nomura Y, Ikawa T, Ogawa T, Miyabe-Nishiwaki T, Kaneko A, Watanabe A, Watanabe S, Hayashi M, Tomonaga M, Hanada N
    • Journal Title

      Int J Syst Evol Microbiol

    • Related Report
      2011 Final Research Report
  • [Journal Article] Human periodontal ligament fibroblasts are the optimal cell source for induced pluripotent stem cells2012

    • Author(s)
      Nomura Y, Ishikawa M, Yashiro Y, Sanggarnjanavanich S, Yamaguchi T, Arai C, Noda K, Takano Y, Nakamura Y, Hanada N
    • Journal Title

      Histochem Cell Biol

    • Related Report
      2011 Final Research Report
  • [Journal Article] Salivary biomarkers for predicting the progression of chronic periodontitis2012

    • Author(s)
      Nomura Y, Shimada Y, Hanada N, Numabe Y, Kamoi K, Sato T, Gomi K, Arai T, Inagaki K, Fukuda M, Noguchi T, Yoshie H
    • Journal Title

      Arch Oral Biol

      Volume: 57(4) Pages: 413-20

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Journal Article] Streptococcus troglodytae sp.nov., from the Chimpanzee Oral Cavity2012

    • Author(s)
      Okamoto M, Imai S, Miyanohara M, Saito W, Momoi Y, Abo T, Nomura Y, Ikawa T, Ogawa T, Miyabe-Nishiwaki T, Kaneko A, Watanabe A, Watanabe S, Hayashi M, Tomonaga M, Hanada N
    • Journal Title

      Int J Syst Evol Microbiol

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Human periodontal ligament fibroblasts are the optimal cell source for induced pluripotent stem cells2012

    • Author(s)
      Nomura Y, Ishikawa M, Yashiro Y, Sanggarnjanavanich S, Yamaguchi T, Arai C, Noda K, Takano Y, Nakamura Y, Hanada N
    • Journal Title

      Histochem Cell Biol.2012

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mechanism of active eruption of molars in adolescent rats2011

    • Author(s)
      Oikawa T, Nomura Y, Arai C, Noda K, Hanada N, Nakamura Y
    • Journal Title

      Eur J Orthod

      Volume: 33(3) Pages: 221-7

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Journal Article] HSPA1A is upregulated in periodontal ligament at early stage of tooth movement in rats2010

    • Author(s)
      Arai C, Nomura Y, Ishikawa M, Noda K, Choi JW, Yashiro Y, Hanada N, Nakamura Y
    • Journal Title

      Histochem Cell Biol

      Volume: 134(4) Pages: 337-43

    • Related Report
      2011 Final Research Report
  • [Journal Article] HSPA1A is upregulated in periodontal ligament at early stage of tooth movement in rats.2010

    • Author(s)
      Arai C, Nomura Y, Ishikawa M, Noda K, Choi JW, Yashiro Y, Hanada N, Nakamura Y.
    • Journal Title

      Histochem Cell Biol.

      Volume: 134(4) Pages: 337-343

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 歯周病患者の特に喫煙と糖尿病に関連した健康意識調査と健康状態に関するアンケート調査2010

    • Author(s)
      長岐祐子, 漆崎絵美, 高野聡美, 三辺正人, 漆原譲治, 野村義明
    • Journal Title

      日本歯周病学会会誌

      Volume: 52巻1号 Pages: 73-82

    • NAID

      10027096206

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Asymmetry of the lips of orthognathic surgery patients.2009

    • Author(s)
      Yamashita Y, Nakamura Y, Shimada T, Nomura Y, Hirashita A.
    • Journal Title

      Am J Orthod Dentofacial Orthop 136(4)

      Pages: 559-563

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Morphological changes in the rat periodontal ligament and its vascularity after experimental tooth movement using superelastic forces.2009

    • Author(s)
      Noda K, Nakamura Y, Kogure K, Nomura Y.
    • Journal Title

      Eur J Orthod. 31(1)

      Pages: 37-45

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] iPS細胞の安全性に関する検討第2報核型異常について2012

    • Author(s)
      野村義明, 石川美佐緒, 新井千博, 八城祐一, 野田晃司, 山口貴央, 阿保備子, 角田衣理加, 山田秀則, 今井奨, 中村芳樹, 花田信弘
    • Organizer
      第61回日本口腔衛生学会総会
    • Place of Presentation
      横須賀市
    • Related Report
      2011 Final Research Report
  • [Presentation] iPS細胞の安全性に関する検討2011

    • Author(s)
      野村義明, 石川美佐緒, 新井千博, 八城祐一, 野田晃司, 山口貴央, 阿保備子, 角田衣理加, 山田秀則, 今井奨, 中村芳樹, 花田信弘
    • Organizer
      第60回日本口腔衛生学会総会
    • Place of Presentation
      松戸市
    • Related Report
      2011 Final Research Report
  • [Presentation] チンパンジー口腔由来のレンサ球菌の性状に関する研究2011

    • Author(s)
      宮之原真由, 今井奨, 齋藤渉, 山口貴央, 阿保備子, 野村義明, 桃井保子, 花田信弘
    • Organizer
      第60回日本口腔衛生学会総会
    • Place of Presentation
      松戸市
    • Related Report
      2011 Final Research Report
  • [Presentation] 唾液検査を用いた歯周病検診受診者の意識と行動の調査2011

    • Author(s)
      花田信弘, 矢吹義秀, 小林憲司, 古藤真実, 福澤洋一, 谷村秀樹, 中曽根隆一, 野村義明
    • Organizer
      第60回日本口腔衛生学会総会
    • Place of Presentation
      松戸市
    • Related Report
      2011 Final Research Report
  • [Presentation] ヒト歯根膜線維芽細胞からのiPS細胞の樹立2011

    • Author(s)
      石川美佐緒, 野村義明, 八城祐一, 新井千博, 山口貴央, 村田貴俊, 野田晃司, 石川雄一, 花田信弘, 中村芳樹
    • Organizer
      第53回歯科基礎医学会総会
    • Place of Presentation
      岐阜市
    • Related Report
      2011 Final Research Report
  • [Presentation] ムタナーゼとデキストラナーゼ両遺伝子の連結によるバイオフィルムの分解2011

    • Author(s)
      角田衣理加, 野村義明, 村田貴俊, 今井奨, 津守秀明, 花田信弘
    • Organizer
      第53回歯科基礎医学会総会
    • Place of Presentation
      岐阜市
    • Related Report
      2011 Final Research Report
  • [Presentation] 細胞への硫化水素暴露により発現が上昇するPHLDA12011

    • Author(s)
      村田貴俊, 角田衣理加, 野村義明, 今井奨, 花田信弘
    • Organizer
      第53回歯科基礎医学会総会
    • Place of Presentation
      岐阜市
    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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