Identification of novel nerve injury-induced proteins and their roles in ion channel modulation and pain transduction

• Project/Area Number: 21600002
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 疼痛学
• Research Institution: Kanazawa University
• Principal Investigator: YOKOYAMA Shigeru 金沢大学, 医学系, 准教授 (00210633)
• Co-Investigator(Kenkyū-buntansha): YOSHIKAWA Hiroaki 金沢大学, 保健管理センター, 教授 (10272981)
• Co-Investigator(Renkei-kenkyūsha): HIGASHIDA Haruhiro 金沢大学, 医学系, 教授 (30093066)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 神経損傷 / イオンチャネル / 痛み

Research Abstract

We explored genes whose protein products were assumed to be released into the extracellular space after axotomy of rat sciatic nerve, and identified glycoprotein non-metastatic melanoma B(Gpnmb) as a candidate. Gpnmb expression was detected in infiltrating macrophages in the nerve ends. Also, Gpnmb was expressed in microglia in the layers I and II of the spinal dorsal horn of normal rats. These results suggest that Gpnmb might be involved in the modulation of pain transduction.

Research Products

• [Journal Article] Expression and immunolocalization of Gpnmb, aglioma-associated glycoprotein, innormal and inflamed central nervous systems of rats (2012)
  • Author(s): Huang, J.-J., Ma, W.-J., and Yokoyama, S
  • Journal Title: Brain and Behavior 2 Volume: 2 Issue: 2 Pages: 85-96
  • DOI: 10.1002/brb3.39
  • Peer Reviewed
• [Journal Article] Interleukin-6 upregulates the expression of PMP22 in cultured rat Schwann cells via a JAK2-dependent pathway (2010)
  • Author(s): Ito, T., Ikeda, K., Tomita, K., and Yokoyama, S
  • Journal Title: Neurosci. Lett Volume: 472 Issue: 2 Pages: 104-108
  • DOI: 10.1016/j.neulet.2010.01.061
  • NAID: 120001939297
  • Peer Reviewed
• [Journal Article] Intracellular calcium elevation induced by extracellular cyclic-ADP-ribose or oxytocin is temperature sensitive in rodent NG108-15 neuronal cells with or without expression of human oxytocin receptors. (2010)
  • Author(s): Amina, S., et al.
  • Journal Title: J.Neuroendocrinol. Volume: 22 Pages: 460-466
  • Peer Reviewed
• [Journal Article] Two genetic variants in CD38 in subjects with autism spectrum disorders and controls. (2010)
  • Author(s): Munesue, T., et al.
  • Journal Title: Neurosci.Res. Volume: 67 Pages: 181-191
  • Peer Reviewed
• [Journal Article] Interleukin-6 upregulates the expression of PMP22 in cultured rat Schwann cells via a JAK2-dependent pathway. (2010)
  • Author(s): Ito T., et al.
  • Journal Title: Neurosci.Lett. 472 Pages: 104-108
  • Peer Reviewed
• [Journal Article] Intracellular calcium elevation induced by extracellular cyclic-ADP-ribose or oxytocin is temperature-sensitive in rodent NG108-15 neuronal cells with or with out expression of human oxytocin receptors. (2010)
  • Author(s): Amina, S., et al.
  • Journal Title: J.Neuroendocrinol.
  • Peer Reviewed
• [Presentation] Expression and Immunolocalization of Gpnmb, a Glioma-Associated Glycoprotein, in Normal and Inflamed Central Nervous Systems of Adult Rats (2012)
  • Author(s): Huang, J.-J., and Yokoyama, S
  • Organizer: 第2回金沢大学子どものこころサミット
  • Place of Presentation: 金沢大学附属病院宝ホール石川県金沢市
  • Year and Date: 2012-03-17
• [Presentation] Expression and Immunolocalization of Gpnmb, a Glioma-Associated Glycoprotein, in Normal and Inflamed Central Nervous Systems of Adult Rats (2012)
  • Author(s): Huang, J.-J., Yokoyama, S.
  • Organizer: 第2回金沢大学子どものこころサミット
  • Place of Presentation: 金沢大学附属病院宝ホール、石川県金沢市
  • Year and Date: 2012-03-17
• [Presentation] Cellular distribution of glycoprotein non-metastatic melanoma B in the rat central nervous system (2011)
  • Author(s): Yokoyama, S., et al
  • Organizer: The 41th Annual Meeting of Scociety for Neuroscience
  • Place of Presentation: Walter E.Washington Convention Center, Washington DC, U.S.A.
  • Year and Date: 2011-11-15
• [Presentation] Expression of Gpnmb, a tumormetastasis-associated glycoprotein, in the rat central nervous system (2011)
  • Author(s): Huang, J.-J., Ma, W.-J., and Yokoyama, S
  • Organizer: 第54回日本神経化学会大会
  • Place of Presentation: ホテル瑠璃光、石川県加賀市
  • Year and Date: 2011-09-27
• [Presentation] CD38 SNPs and autism. (2011)
  • Author(s): Yokoyama, S.
  • Organizer: Meeting for the World-class Research Hub in East Asia on cADPR and CD38(Winter Conference in Kanazawa2011)
  • Place of Presentation: ホテル瑠璃光、石川県却賀市(招待講演)
  • Year and Date: 2011-02-24
• [Presentation] Cellular distribution of glycoprotein non-metastatic melanoma B in the ratcentral nervous system (2011)
  • Author(s): Yokoyama, S., and Huang, J.-J
  • Organizer: The 41th Annual Meeting of Scociety for Neuroscience
  • Place of Presentation: Walter E. Washington Convention Center, Washington DC, MD, U. S. A.
• [Presentation] Expression of glycoprotein non-metastatic melanoma B in the rat central nervous system. (2010)
  • Author(s): Huang, J.-J., et al.
  • Organizer: The 40th Annual Meeting of Society for Neuroscience
  • Place of Presentation: San Diego Convention Center, San Diego, CA, U.S.A.
  • Year and Date: 2010-11-15
• [Presentation] Two single nucleotide polymorphism in CD38 : A relation to high-functioning autism or autism. (2010)
  • Author(s): Yokoyama, S., et al.
  • Organizer: A Brain Research meeting : The Emerging Neuroscience of Autism Spectrum Disorders : Etiologic Insights ; Treatment Opportunities
  • Place of Presentation: Westin Hotel, San Diego, CA, U.S.A.
  • Year and Date: 2010-11-11
• [Presentation] Improved social communication after nasal oxytocin administration in a Japanese autistic patient with severe mental retardation. (2010)
  • Author(s): Higashida, H., et al.
  • Organizer: A Brain Research meeting : The Emerging Neuroscienceof Autism Spectrum Disorders : Etiologic Insights ; Treatment Opportunities
  • Place of Presentation: Westin Hotel, San Diego, CA, U.S.A.
  • Year and Date: 2010-11-11
• [Presentation] 達物質受容体・イオンチャネルの疼痛発生への関与 (2010)
  • Author(s): 横山茂
  • Organizer: 第23回がん診療拠点病院研修会「緩和ケア講習会」
  • Place of Presentation: 金沢医科大学石川県河北郡内灘町
  • Year and Date: 2010-06-07
• [Presentation] 神経伝達物質受容体・イオンチャネルの疹痛発生への関与 (2010)
  • Author(s): 禎山茂
  • Organizer: 第23回がん診搬点病院研修会「緩和ケア講習会」
  • Place of Presentation: 金沢医科大学、石川県河北郡内灘町(招待講演)
  • Year and Date: 2010-06-07
• [Presentation] Expression of glycoproteinnon-metastatic melanoma B in the ratcentral nervous system (2010)
  • Author(s): Huang, J.-J., Ma, W.-J., and Yokoyama, S
  • Organizer: The 40th Annual Meeting of Scociety for Neuroscience
  • Place of Presentation: San Diego Convention Center, San Diego, CA, U. S. A.
• [Presentation] Expression of Gpnmb, a tumor metastasis-associated glycoprotein, in the rat central nervous system (2009)
  • Author(s): Huang, J.-J., Ma, W.-J., Higashida, H., and Yokoyama, S
  • Organizer: 第56回中部日本生理学会
  • Place of Presentation: 石川県立音楽堂交流ホール石川県金沢市
  • Year and Date: 2009-12-04
• [Presentation] CD157と脳機能 (2009)
  • Author(s): 東田陽博, 他
  • Organizer: 第56回中部日本生理学会
  • Place of Presentation: 石川県立音楽堂交流ホール(石川県)
  • Year and Date: 2009-12-04
• [Presentation] Expression of gpnmb, a tumor metastasis-associated glycoprotein, in the rat central nervous system. (2009)
  • Author(s): Huang, J.-J., et al.
  • Organizer: 第56回中部日本生理学会
  • Place of Presentation: 石川県立音楽堂交流ホール(石川県)
  • Year and Date: 2009-12-04
• [Presentation] 自閉症スペクトラム症例におけるCD38遺伝子の多型解析 (2009)
  • Author(s): 横山茂
  • Organizer: 金沢大学創基150周年記念「講演会・シンポジウム」シリーズ第2回「社会認識」学際脳科学シンポジウム
  • Place of Presentation: 金沢大学医学部記念館(石川県)
  • Year and Date: 2009-11-06
• [Presentation] Expression of gpnmb, a tumor metastasis-associated glycoprotein, in the rat central nervous system. (2009)
  • Author(s): Huang, J.-J., et al.
  • Organizer: 金沢大学創基150周年記念「講演会・シンポジム」シリーズ第2回「社会認識」学際脳科学シンポジウム
  • Place of Presentation: 金沢大学医学部記念館(石川県)
  • Year and Date: 2009-11-05
• [Presentation] Identification and characterization of a novel agrin variant with an alternative carboxyl terminus. (2009)
  • Author(s): Yoshikawa, K., et al.
  • Organizer: The 39th Annual Meeting of Scociety for Neuroscience
  • Place of Presentation: The McCormick Place Convention Center(Chicago, U.S.A.)
  • Year and Date: 2009-10-21
• [Presentation] Oxytocin levels in adult and infant CD38 knockout mice critically relate to social behavior. (2009)
  • Author(s): 東田陽博, et al.
  • Organizer: 19th Neuropharmacology Conference
  • Place of Presentation: Hyatt Regacy McCormick Place(Chicago, U.S.A.)
  • Year and Date: 2009-10-14
• [Presentation] Expression and characterization of human oxytocin receptor in HEK293 cells. (2009)
  • Author(s): Ma, W.-H., et al.
  • Organizer: The 8th World Congress on Neurohypophysial Hormones(WCNH2009)
  • Place of Presentation: Kitakyushu International Conference Center(福岡県)
  • Year and Date: 2009-09-05
• [Presentation] Collagen type VI : apotential nerve regenerating factor (2009)
  • Author(s): 横山茂
  • Organizer: カザン国立大学物理学部と金沢大学医薬保健研究域との共同研究に関する包括的情報交換会
  • Place of Presentation: 金沢大学医薬保健研究域医学類B棟1階小会議室石川県金沢市
  • Year and Date: 2009-09-01
• [Presentation] Collagen type VI : a potential nerve regenerating factor. (2009)
  • Author(s): 横山茂
  • Organizer: カザン国立大学物理学部と金沢大学医薬保健研究域との共同研究に関する包括的情報交換会
  • Place of Presentation: 金沢大学(石川県)
  • Year and Date: 2009-09-01
• [Presentation] Expression of Gpnmb, a tumor metastasis-associated glycoprotein, in the rat central nervous system (2009)
  • Author(s): Huang, J.-J., Ma, W.-J., Higashida, H., and Yokoyama, S
  • Organizer: 金沢大学創基150周年記念「講演会・シンポジウム」シリーズ第2回「社会認識」学際脳科学シンポジウム
  • Place of Presentation: 金沢大学医学部記念館石川県金沢市
• [Presentation] Identification and characterization of a novel agrin variant with an alternative carboxyl terminus (2009)
  • Author(s): Yoshikawa, K., Yoshikawa, H., and Yokoyama, S
  • Organizer: The 39th Annual Meeting of Scociety for Neuroscience
  • Place of Presentation: The McCormick Place Convention Center, Chicago, U. S. A.
• [Book] Injuries and Regeneration (2011)
  • Author(s): Yokoyama, S. Fonseca, D. J., and Martins, J. L.
  • Publisher: Nova Science Publishers Inc.
• [Book] The Sciatic Nerve : Blocks, Injuries and Regeneration (2011)
  • Author(s): Yokoyama, S.
  • Publisher: Nova Science Publishers Inc
• [Patent(Industrial Property Rights)] 心筋障害の検査方法 (2010)
  • Inventor(s): 吉川弘明, 横山茂, 他
  • Industrial Property Rights Holder: 金沢大学
  • Industrial Property Number: 2010-036741
  • Filing Date: 2010-02-23
• [Patent(Industrial Property Rights)] アセチルコリン受容体クラスター形成阻害剤 (2010)
  • Inventor(s): 吉川弘明, 横山茂, 他
  • Industrial Property Rights Holder: 金沢大学
  • Industrial Property Number: 2010-036740
  • Filing Date: 2010-02-23
• [Patent(Industrial Property Rights)] アセチルコリン受容体クラスター形成阻害活性を有するアグリンを特異的に認識する抗体並びに該抗体を含むアセチルコリン受容体クラスター形成能促進剤及び該抗体を充填したアセチルコリン受容体クラスター形成能を阻害するアグリン除去カラム (2010)
  • Inventor(s): 吉川弘明, 横山茂, 丸田高広
  • Industrial Property Rights Holder: 金沢大学
  • Industrial Property Number: 2010-036739
  • Filing Date: 2010-02-23