Elucidation of the mechanisms of nociceptor excitation by noxious temperature

• Project/Area Number: 21600004
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 疼痛学
• Research Institution: Nagoya University
• Principal Investigator: KATANOSAKA Kimiaki 名古屋大学, 環境医学研究所, 助教 (50335006)
• Co-Investigator(Kenkyū-buntansha): KATANOSAKA Yuki 岡山大学, 大学院・医歯薬学総合研究科, 助教 (60432639)
• Co-Investigator(Renkei-kenkyūsha): MIZUMURA Kazue 中部大学, 生命健康科学部, 教授 (00109349) ; TAGUCHI Toru 名古屋大学, 環境医学研究所, 助教 (90464156) ; KATANOSAKA Yuki 岡山大学, 大学院・医歯薬学総合研究科, 助教 (60432639)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 温度受容 / 痛覚 / TRPチャネル / 侵害受容器 / 一次知覚ニューロン / 温度感覚 / 熱 / pERK / 痛み / 温度 / 感覚 / 受容体 / 冷痛覚過敏 / TRPV2

Research Abstract

The mechanisms of heat-and cold-nociception in the nociceptive sensory neurons were examined in rat and mice. No clear correlation between temperature sensitivities of the primary sensory neurons in dorsal root ganglion(DRG) and expression of molecular candidates for nociceptive cold and heat receptors, such as TRPA1 and V2channels, was observed in cultured DRG neurons and anesthetized animals. The data suggested the involvement of other mechanisms independent of these candidate molecules. In addition, I have improved a cold pain assay(cold plate test) to clearly observe the cold pain-related behavior in normal rats and cold hyperalgesia in inflamed rats.

Research Products

• [Journal Article] TRPV3 in keratinocytes transmits temperature information to sensory neurons via ATP. (2009)
  • Author(s): Mandani S, Sokabe T, Shibasaki K, Katanosaka K, Mizuno A, Moqrich A, Patapoutian A, Fukumi-Tominaga T, Mizumura K, Tominaga M
  • Journal Title: Pflugers Archiv-European Journal of Physiology Volume: 458(6) Pages: 1093-1102
  • Peer Reviewed
• [Journal Article] Excitation and sensitization of nociceptors by bradykinin : What do we know? (2009)
  • Author(s): Mizumura K, Sugiura T, Katanosaka K, Banik RK, Kozaki Y
  • Journal Title: Experimental Brain Research Volume: 196(1) Pages: 53-65
• [Presentation] Role of TRPV1 in mechanically induced pain (2011)
  • Author(s): Murase S, Ota H, Katanosaka K, KuboA, Queme F, Matsuda T, Taguchi T, Mizumura K
  • Organizer: International Symposium on Mechano-biology(The Fifth Shanghai International Conference on Biophysics and Molecular biology)
  • Place of Presentation: Huashia Hotel(Shanghai, CHINA)
  • Year and Date: 2011-11-05
• [Presentation] Role of TRPV1 in mechanically induced pain (2011)
  • Author(s): Murase S, Ota H, Katanosaka K, Kubo A, Queme F, Matsuda T, Taguchi T, Mizumura K
  • Organizer: International Symposium on Mechanobiology (The Fifth Shanghai International Conference on Biophysics and Molecular biology)
  • Place of Presentation: Huashia Hotel (Shanghai, CHINA)(招待講演)
  • Year and Date: 2011-11-05
• [Presentation] The chemical sensitivities and expression of cold-sensitive ionchannels in sensory neurons in the persistently inflamed rat (2011)
  • Author(s): Katanosaka K, Ota T, Hirose T, MoriK, Mizumura K
  • Organizer: The 8th International Congress on Comparative Physiology and Biochemistry
  • Place of Presentation: Nagoya Congress Center(Nagoya, JAPAN)
• [Presentation] The chemical sensitivities and expression of cold-sensitive ion channels in sensory neurons in the persistently inflamed rat (2011)
  • Author(s): Katanosaka K, Ota T, Hirose T, Mori K, Mizumura K
  • Organizer: The 8th International Congress on Comparative Physiology and Biochemistry
  • Place of Presentation: Nagoya Congress Center (Nagoya, JAPAN)
• [Presentation] 繰り返し寒冷ストレスによる機械痛覚過敏における性差 (2010)
  • Author(s): 那須輝顕,片野坂公明,水村和枝
  • Organizer: 第49回日本生気象学会大会
  • Place of Presentation: 文化女子大学(東京)
  • Year and Date: 2010-11-05
• [Presentation] Sex difference of repeated cold stress(RCS)-induced cutaneous and muscular mechanical hyperalgesia in rats (2010)
  • Author(s): Nasu T, Katanosaka K, Mizumura K
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡市民文化ホール(盛岡)
  • Year and Date: 2010-05-20
• [Presentation] Sex difference of repeated cold stress(RCS)-induced cutaneous and muscular mechanical hyperalgesia in rats. (2010)
  • Author(s): NASU Teruaki, KATANOSAKA, Kimiaki, MIZUMURA Kazue
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡市民文化ホール(盛岡)
  • Year and Date: 2010-05-20
• [Presentation] プロスタグランジンはEP3受容体を介して、内在化したブラジキニンB2受容体のリサイクリングに作用する可能性がある (2010)
  • Author(s): Kozaki Y, Suzuki Y, Katanosaka K, Mori M, Mizumura K
  • Organizer: 第33回日本神経科学会(Neuro 2010)
  • Place of Presentation: 神戸コンベンションセンター(神戸)
• [Presentation] プロスタグランジンEP3受容体の活性化による内在化ブラジキニンB2受容体の細胞膜へのリサイクリングの促進 (2010)
  • Author(s): 小崎康子,鈴木義明,片野坂公明,森雅美,水村和枝
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山コンベンションセンター(岡山)
• [Presentation] Behavioral hyper-sensitivity to noxious cold and functional changes of DRG neurons of the rats suffering from persistent joint inflammation (2009)
  • Author(s): Katanosaka K, Ota T, Hirose T, MoriK, Mizumura K
  • Organizer: The 36th Congress of the International Union of Physiological Sciences(IUPS2009)
  • Place of Presentation: Kyoto International Conference Center(KYOTO, JAPAN)
  • Year and Date: 2009-07-29
• [Presentation] Behavioral hypersensitivity to noxious cold and functional changes of DRG neurons of the rats suffering from persistent joint inflammation (2009)
  • Author(s): Katanosaka K, Ota T, Hirose T, Mori K, Mizumura K
  • Organizer: The 36th Congress of the International Union of Physiological Sciences(IUPS2009)
  • Place of Presentation: KYOTO