Clarification of dorsal root evoked synaptic responses in lamina I neurons

• Project/Area Number: 21600005
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 疼痛学
• Research Institution: Kumamoto Health Science University
• Principal Investigator: YOSHIMURA Megumu 熊本保健科学大学, 大学院・保健科学研究科, 教授 (10140641)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: slow EPSP / サブスタンスP / 後角第I層 / 投射ニューロン / 脊髄第I層 / 緩徐シナプス応答 / C線維 / ペプチド / 痛覚伝達 / 脊髄後角 / 痛覚過敏 / 脊髄スライス / slow EPSC / 痛覚 / 脊髄スライス標本 / 無髄のC線維

Research Abstract

Intracellular recordings were made from lamina I neurons which were identified by antidromic action potentials evoked by focal stimulation near the central canal. Stimulation of the dorsal root elicited a fast EPSP in the majority of neurons and repetitive stimulation evoked a slow EPSP in 20% of lamina I neurons. The latter neurons were depolarized by application of substance P and antagonized by a NK1 receptor antagonist, suggesting that the lamina I neurons receive substance P containing C afferent fibers which could modulate the nociceptive transmission in the lamina I.

Research Products

• [Journal Article] Analgesic effect of calcitonin on neuropathic pain interacting with peripheral calcitonin receptors (2012)
  • Author(s): Akitoshi Ito, Mineko Takeda, Takeshi Yoshimura, Takayuki Komatsu, Takeshi Ohno, Hiroshi Kuriyama, Akio Matsuda and Megumu Yoshimura
  • Journal Title: Molecular Pain
• [Journal Article] Identification of 5-HT receptor subtypes enhancing inhibitory transmission in the rat spinal dorsal horn in vitro (2012)
  • Author(s): Du-Jie Xie, Daisuke Uta, Peng-Yu Feng, Masahito Wakita, Min-Chul Shin, Hidemasa Furue and Megumu Yoshimura
  • Journal Title: Molecular Pain
• [Journal Article] GABAA receptor-mediated presynaptic inhibition on glutamatergic transmission (2011)
  • Author(s): Yamamoto S, Yoshimura M, Shin M-C, Wakita M, Nonaka K, Akaike N.
  • Journal Title: Brain Research Bulletin Volume: 84 Pages: 22-30
• [Journal Article] Bone cancer pain induces a unique central sensitization through synaptic changes in a wide area of the spinal cord (2010)
  • Author(s): Yanagisawa Y, Furue H, Kawamata T, Uta D, Yamamoto J, Furuse S, Katfuchi T, Imoto K, Iwamoto Y, Yoshimura M.
  • Journal Title: Molecular Pain Volume: 6 Pages: 38-38
• [Journal Article] Responsiveness of C neurons in rat dorsal root ganglion to 5-hydroxytryptamine-induced pruritic stimuli in vivo (2010)
  • Author(s): Hachisuka H, Furue H, Furue M, Yoshimura M
  • Journal Title: Journal of Neurophysiology Volume: 104(1) Pages: 271-279
• [Journal Article] TRPA1-expressing primary afferents synapse with morphologically identifie113d subtypes of substantia gelatinosa neurons in the adult rat spinal cord (2010)
  • Author(s): Uta D, Furue H, Pickering AE, Rashid MDH, Mizuguchi-Takase H, Katafuchi T, Yoshimura M
  • Journal Title: European Journal of Neuroscience Volume: 31(11) Pages: 1960-1973
• [Journal Article] Enhancement of GABAergic tonic currents by midazolam and noradrenaline in rat substantia gelantinosa neurons in vitro (2010)
  • Author(s): Maeda A, Katafuchi T, Oba Y, Shiokawa H, Yoshimura M.
  • Journal Title: Anesthesiology Volume: 113(2) Pages: 429-437
• [Journal Article] TRPA1-expressing primary afferents synapse with morphologically identifird subtypes f substantia gelatinosa neurons in the adult rat spinal cord. (2010)
  • Author(s): Uta D, Furue H, Pickering AE, Rashid MDH, Mizuguchi-Takase H, Katafuchi T, Yoshimura M.
  • Journal Title: European Journal of Neuroscience. (In press)
  • Peer Reviewed
• [Journal Article] Enhancement of GABAergic tonic currents by midazolam and noradrenaline in rat substantia gelantinosa neurons in vitro (2010)
  • Author(s): Maeda A, Katafuchi T, Oba Y, Shiokawa H, Yoshimura M.
  • Journal Title: Anesthesiology (In press)
  • Peer Reviewed
• [Journal Article] Organization of intra-and translaminar neuronal connectivity in the superficial spinal dorsal horn (2009)
  • Author(s): Kato G, Kawasaki Y, Koga K, Uta D, Kosugi M, Yasaka T, Yoshimura M, Ji RR, Strassman AM.
  • Journal Title: J Neurosci Volume: 29(16) Pages: 5088-5099
• [Journal Article] Cytokine-induced suppression of medial preoptic neurons. Mechanisms and neuroimmunomodulatory effects (2009)
  • Author(s): Katafuchi T, Duan S, Take S, Yoshimura M
  • Journal Title: Neuroimmunomodulation : Ann. N. Y. Acad. Sci Volume: 1153 Pages: 76-81
• [Journal Article] Actions of propofol on substantia gelatinosa neurones in rat spinal cord revealed by in vitro and in vivo patch-clamp recordings (2009)
  • Author(s): Takazawa T, Furue H, Nishikawa K, Uta D, Takeshima K, Goto F, Yoshimura M
  • Journal Title: European Journal of Neuroscience Volume: 29(3) Pages: 518-28
• [Journal Article] Responses to 5-HT morphologically identified neurons in the rat substantia gelatinosa in vitro (2009)
  • Author(s): Abe K, Kato G, Katafuchi T, Tamae A, Furue H and Yoshimura M
  • Journal Title: Neuroscience Volume: 159(1) Pages: 316-324
• [Journal Article] Organization of intra- and translaminar neuronal connectivity in the superficial spinal dorsal horn (2009)
  • Author(s): Kato G, Kawasaki Y, Koga K, Uta D, Kosugi M, Yasaka T, Yoshimura M, Ji RR, Strassman AM.
  • Journal Title: Journal of Neuroscience 29(16) Pages: 5088-5099
  • Peer Reviewed
• [Journal Article] Actions of propofol on substantia gelatinosa neurones in rat spinal cord revealed by in vitro and in vivo patch-clamp recordings (2009)
  • Author(s): Takazawa T, Furue H, Nishikawa K, Uta D, Takeshima K, Goto F, Yoshimura M.
  • Journal Title: European Journal of Neuroscience 29(3) Pages: 518-528
  • Peer Reviewed
• [Journal Article] Responses to 5-HT morphologically identified neurons in the rat substantia gelatinosa in vitro (2009)
  • Author(s): Abe K, Kato G, Katafuchi T, Tamae A, Furue H, Yoshimura M.
  • Journal Title: Neuroscience 159(1) Pages: 316-324
  • Peer Reviewed
• [Presentation] 脊髄後角第I層投射ニューロンの後根誘起slow EPSPの発生機序 (2012)
  • Author(s): 吉村惠
  • Organizer: 新潟大学大学院セミナー
  • Place of Presentation: 新潟大学医学部(新潟)
  • Year and Date: 2012-03-09
• [Presentation] Sensitization of TRPV1 channel expressed at the primary C afferent terminals by inflammation in the rat spinal dorsal horn (2011)
  • Author(s): Yoshimura M, Xie D, Uta D, Furue H
  • Organizer: 4th Asiian Pain Symposium
  • Place of Presentation: Shanghai, China
• [Presentation] (2011)
  • Author(s): 吉村惠
  • Organizer: 九州痲酔学会特別講演
  • Place of Presentation: 福岡,日本
• [Presentation] In vivo analysis of 5-HT-induced pruritic responses in rat DRG C neurons (2010)
  • Author(s): Yoshimura M, J Hachisuka J, Furue H, Furue M
  • Organizer: 日本神経科学大会シンポジウム
  • Place of Presentation: 神戸、日本
• [Presentation] 慢性炎症性疼痛発生におけるBDNFの関与と脊髄内感覚回路の可塑性 (2010)
  • Author(s): 吉村惠
  • Organizer: 情報技術協会シンポジウム
  • Place of Presentation: 東京、日本
• [Presentation] 痛みを考えるー生理学的研究からみた慢性疼痛の発生機序ー (2010)
  • Author(s): 吉村惠
  • Organizer: 日本カイロプラスティック学会特別講演
  • Place of Presentation: 福岡、日本
• [Presentation] Functional role of the TRPV1 receptors expressed at the terminal of prmary C afferent in the spinal dorsal hor (2010)
  • Author(s): Yoshimura M, Xie D, Furue H, Hachisuka J, Uta D
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜、日本、シンポジウム
• [Presentation] Synaptic connection in the superficial dorsal horn revealed by laser photostimulation (2010)
  • Author(s): Yoshimura M, Uta D, Furue H, Hachisuka Y
  • Organizer: 9^<th> Japan. Korea Joint Symposium
  • Place of Presentation: Kagoshima
• [Presentation] The plastic changes of spinal excitatory synaptic transmission in a murine model of cancer-induced bone pain (2010)
  • Author(s): Yanagisawa Y, Furue H, Kawamata T, Uta D, Imoto K, Iwamoto Y, Yoshimura M
  • Organizer: Society for Neuroscience
  • Place of Presentation: San Diego
• [Presentation] Effects of acupucture on the inhibitory transmission in the spinal cord (2009)
  • Author(s): Yoshimura M
  • Organizer: The 36^<th> Congress of the International Union of Physiological Sciences
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2009-07-29
• [Presentation] Responsiveness of C neurons in rat DRG and spinal dorsal horn neruons to 5-HT induced itch stimulation in vivo (2009)
  • Author(s): Yoshimura M
  • Organizer: 4^<th> International Conference of Neurons and Brain Diseases
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2009-07-22
• [Presentation] Enhancement of excitatory synaptic transmission in the substantia gelatinosa of the spinal cord in a mouse model of bone cancer pain (2009)
  • Author(s): Yanagisawa Y, Furue H, Kawamata T, Uta D, Iwamoto Y, Imoto K, Yoshimura M
  • Organizer: Society for Neuroscience
  • Place of Presentation: Chicago
• [Presentation] Actions of TRPA1 and TRPV1 agonists on synaptic transmission in substantia gelatinosa neurons of the adult rat spinal cord (2009)
  • Author(s): Uta D, Furue H, Imoto K, Yoshimura M.
  • Organizer: Society for Neuroscience
  • Place of Presentation: Chicago
• [Presentation] Actions of endothelin-1 on nociceptive transmission in substantia gelatinosa of the adult mouse spinal cord. (2009)
  • Author(s): Nishikawa Y, Uta D, Sasaki A, Andouh T, Furue H, Yoshimura M, Kuraishi Y
  • Organizer: Society for Neuroscience
  • Place of Presentation: Chicago
• [Presentation] Effects of acupucture on the inhibitory transmission in the spinal cord (2009)
  • Author(s): Yoshimura M
  • Organizer: The 36^<th> Congress of the International Union of Physiological Sciences
  • Place of Presentation: Kyoto(Symposium)
• [Presentation] Comprehensive approaches to pain-from molecular to organism- (2009)
  • Author(s): Yoshimura M. Mizumura K.
  • Organizer: The 36^<th> Congress of the International Union of Physiological Sciences
  • Place of Presentation: Kyoto(Chair)
• [Presentation] Sensory Neural network formation with RNA binding protein musashi2 deficient mice (2009)
  • Author(s): Shibata S, Sakakibara S-I, Furue H, Yoshimura M, Takagi T, Ohkuma R, Kuwako K-I, Okano H, Okano H
  • Organizer: The 36^<th> Congress of the International Union of Physiological Sciences
  • Place of Presentation: Kyoto
• [Presentation] Modulation of nociceptive synaptic transmission in the adult rat spinal dorsal horn via activation of TPA1 and TRPV1 (2009)
  • Author(s): Uta D, Furue H, Yoshimura M
  • Organizer: The 36^<th> Congress of the International Union of Physiological Sciences.
  • Place of Presentation: Kyoto
• [Presentation] Selective loss of presynaptic 5-HT inhibitory actions on spinal mechanical noxious synaptic transmission in ovariectomized rats in vivo (2009)
  • Author(s): Furue H, Ito A, Yoshimura M
  • Organizer: The 36^<th> Congress of the International Union of Physiological Sciences
  • Place of Presentation: Kyoto
• [Presentation] Plastic changes in spinal nociceptive transmission of bone cancer pain model mice (2009)
  • Author(s): Yanagisawa Y, Furue H, Kawamata T, Namiki A, Iwamoto Y, Yoshimura M
  • Organizer: The 36^<th> Congress of the International Union of Physiological Sciences
  • Place of Presentation: Kyoto
• [Presentation] Neuronal circuitries and receptors involved in spinal cord pain processing (2009)
  • Author(s): Andrew J. Todd, Yoshimura M
  • Organizer: Nagoya Pain 2009
  • Place of Presentation: Nagoya
• [Presentation] Responsiveness of C neurons in rat DRG and spinal dorsal horn neruons to 5-HT induced itch stimulation in vivo (2009)
  • Author(s): Yoshimura M
  • Organizer: 4^<th> International Conference of Neurons and Brain Diseases
  • Place of Presentation: Toronto(Symposium)
• [Presentation] Sensory Neural network formation with RNA binding protein musashi2 deficient mice. (2009)
  • Author(s): Shibata S, Sakakibara S-I, Furue H, Yoshimura M, Takagi T, Ohkuma R, Kuwako K-I, Okano H, Okano H.
  • Organizer: The 36^<th> Congress of the International Union of Physiological Sciences
  • Place of Presentation: Kyoto, Japan
• [Presentation] Modulation of nociceptive synaptic transmission in the adult rat spinal dorsal horn via activation of TPA1 and TRPV1 (2009)
  • Author(s): Uta D, Furue H, Yoshimura M.
  • Organizer: The 36^<th> Congress of the International Union of Physiological Sciences
  • Place of Presentation: Kyoto, Japan
• [Presentation] Selective loss of presynaptic 5-HT inhibitory actions on spinal mechanical noxious synaptic transmission in ovariectomized rats in vivo (2009)
  • Author(s): Furue H, Ito A, Yoshimura M
  • Organizer: The 36^<th> Congress of the International Union of Physiological Sciences
  • Place of Presentation: Kyoto, Japan
• [Presentation] Enhancement of excitatory synaptic transmission in the substantia gelatinosa of the spinal cord in a mouse model of bone cancer pain (2009)
  • Author(s): Yanagisawa Y, Furue H, Kawamata T, Uta D, Iwamoto Y, Imoto K, Yoshimura M
  • Organizer: Society for Neuroscience
  • Place of Presentation: Chicago, USA
• [Presentation] Actions of TRPA1 and TRPV1 agonists on synaptic transmission in substantia gelatinosa neurons of the adult rat spinal cord (2009)
  • Author(s): Uta D, Furue H, Imoto K, Yoshimura M
  • Organizer: Society for Neuroscience
  • Place of Presentation: Chicago, USA
• [Presentation] Actions of endothelin-1 on nociceptive transmission in substantia gelatinosa of the adult mouse spinal cord (2009)
  • Author(s): Nishikawa Y, Uta D, Sasaki A, Andouh T, Furue H, Yoshimura M, Kuraishi Y
  • Organizer: Society for Neuroscience
  • Place of Presentation: Chicago, USA
• [Book] 標準生理学、体性感覚 (2009)
  • Author(s): 吉村惠(分担)
  • Publisher: 医学書院