Analysis of glial-progenitor-neural networks and role of nitric oxide to maintain neuropathic pain

• Project/Area Number: 21600015
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 疼痛学
• Research Institution: Kansai Medical University
• Principal Investigator: MATSUMURA Shinji 関西医科大学, 医学部, 講師 (70276393)
• Co-Investigator(Kenkyū-buntansha): ITO Seiji 関西医科大学, 医学部, 教授 (80201325)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 神経障害性疼痛 / 脊髄 / 神経可塑生 / 前駆細胞 / グリア / プリン作動性 / マウス / 神経可塑性 / 5-bromodeoxyuridine / 神経因性疼痛 / プリン作動生

Research Abstract

Nestin is a class VI intermediate filament expressed in NPCs. To study whether neurons and glia proliferate in situ or migrate from specific regions harboring neural progenitor cells, we applied L5 spinal nerve transection to nestin-promoter GFPtransgenic mice to narrow the target to neural progenitor cells. It was suggested that proliferation of neural progenitor cells and their differentiation into neurons and astrocytes occur in the dorsal horn in response to peripheral nerve injury and that they may contribute to the maintenance of neuropathic pain at the late phase after peripheral nerve injury.

Research Products

• [Journal Article] 痛みの可塑性と慢性化 (2011)
  • Author(s): 伊藤誠二、下條正仁、松村伸治
  • Journal Title: 脊椎脊髄ジャーナル特集痛みとしびれのサイエンス-基礎と臨床 Volume: 第24巻第5号 Pages: 341-347
• [Journal Article] A novel role of prostaglandin E2 in neuropathic pain : blockade of microglial migration in the spinal cord (2011)
  • Author(s): Kunori S, Matsumura S, Okuda-Ashitaka E, Katano T, Audoly LP, Urade Y, Ito S
  • Journal Title: Glia Volume: 59 Issue: 2 Pages: 208-218
  • DOI: 10.1002/glia.21090
  • Peer Reviewed
• [Journal Article] 痛みの可塑性と慢性化 (2011)
  • Author(s): 伊藤誠二、下條正仁、松村伸治
  • Journal Title: 脊椎脊髄ジャーナル Volume: 24 Pages: 341-347
• [Journal Article] A novel role of prostaglandin E2 in neuropathic pain : blockade of microglial migration in the spinal cord (2010)
  • Author(s): Kunori, S., Matsumura, S., Okuda-Ashitaka, E., Katano, T., Audoly, L., Urade, Y. and Ito, S
  • Journal Title: Glia Volume: 59 Pages: 208-218
  • Peer Reviewed
• [Journal Article] Impairment of CaMKII activation and attenuation of neuropathic pain in mice lacking NR2B phosphorylated at Tyr1472 (2010)
  • Author(s): Matsumura, S., Kunori, S., Mabuchi, T., Katano, T., Nakazawa, T., Abe, T., Watanabe, M., Yamamoto, T., Okuda-Ashitaka, E. and Ito, S
  • Journal Title: Eur. J. Neurosci Volume: 32 Pages: 798-810
  • Peer Reviewed
• [Journal Article] Characterization of nestin expression in the spinal cord of GFP transgenic mice after peripheral nerve injury (2010)
  • Author(s): Matsumura, S., Takagi, K., Okuda-Ashitaka, E., Lu, J., Naritsuka, H., Yamaguchi, M. and Ito, S
  • Journal Title: Neuroscience Volume: 170 Pages: 942-953
  • Peer Reviewed
• [Journal Article] Characterization of nestin expression in the spinal cord of GFP transgenic mice after peripheral nerve injury. (2010)
  • Author(s): Matsumura S, Takagi K, Okuda-Ashitaka E, Lu J, Naritsuka H, Yamaguchi M, Ito S.
  • Journal Title: Neuroscience Volume: 170 Pages: 942-953
  • Peer Reviewed
• [Journal Article] Impairment of CaMKII activation and attenuation of neuropathic pain in mice lacking NR2B phosphorylated at Tyr1472. (2010)
  • Author(s): Matsumura S, Kunori S, Mabuchi T, Katano T, Nakazawa T, Abe T, Watanabe M, Yamamoto T, Okuda-Ashitaka E, Ito S.
  • Journal Title: European Journal of Neuroscience Volume: 32 Pages: 798-810
  • Peer Reviewed
• [Journal Article] A novel role of prostaglandin E_2 in neuropathic pain : blockade of microglial migration in the spinal cord. (2010)
  • Author(s): Kunori S, Matsumura S, Okuda-Ashitaka E, Katano T, Audoly LP, Urade Y, Ito S.
  • Journal Title: Glia Volume: 59 Pages: 208-218
  • Peer Reviewed
• [Journal Article] Translocation of neuronal nitric oxide synthase to the plasma membrane by ATP is mediated by P_2X and P_2Y receptors (2009)
  • Author(s): Ohnishi, T., Matsumura, S. and Ito, S
  • Journal Title: Mol. Pain Volume: 5
  • Peer Reviewed
• [Journal Article] Involvement of prostaglandin F_<2α> receptor in ATP-induced mechanical allodynia (2009)
  • Author(s): Kunori, S., Matsumura, S., Mabuchi, T., Tastumi, S., Sugimoto, Y., Minami, T. and Ito, S
  • Journal Title: Neuroscience Volume: 163 Pages: 362-371
  • Peer Reviewed
• [Journal Article] Translocation of neuronal nitric oxide synthase to the plasma membrane by ATP is mediated by P2X and P2Y receptors (2009)
  • Author(s): Ohnishi T, Matsumura S, Ito S
  • Journal Title: Molecular Pain 5
  • Peer Reviewed
• [Journal Article] Involvement of prostaglandin F 2 alpha receptor in ATP-induced mechanical allodynia (2009)
  • Author(s): Kunori S, Matsumura S, Mabuchi T, Tatsumi S, Sugimoto Y, Minami T, Ito S
  • Journal Title: Neuroscience 163 Pages: 362-371
  • Peer Reviewed
• [Journal Article] Effects of neurotropic factors on nerve regeneration monitored by in vivo imaging in thyl-YFP transgenic mice (2009)
  • Author(s): Unezaki, S., Yoshii, S., Mabuchi, T., Saito, A., Ito, S
  • Journal Title: Journal Neuroscience Methods 178 Pages: 308-315
  • Peer Reviewed
• [Presentation] Blockade of microglial migration in the spinal cord by prostaglandin E2 via EP2 (2011)
  • Author(s): Ito, S., Kunori, S., Matsumura, S., Katano, T., Urade, Y. and Okuda-Ashitaka, E
  • Organizer: The 41th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: Washington
• [Presentation] Attenuation of neuropathic pain by impaired Ca^<2+> signaling in mutantmice lacking the Tyr-1472 phosphorylation site of the NR2B (2010)
  • Author(s): Matsumura S, Kunori S, Nakazawa T, Abe T, Yamamoto T, Katano T, Watanabe M, Okuda-Ashitaka E, Ito S
  • Organizer: Society for Neuroscience
  • Place of Presentation: Sandiego,米国
  • Year and Date: 2010-11-15
• [Presentation] Attenuation of neuropathic pain by impaired Ca2+signaling in mutant mice lacking the Tyr-1472 phosphorylation site of the NR2B (2010)
  • Author(s): Matsumura, S., Kunori, S., Nakazawa, T., Abe, T., Yamamoto, T., Katano, T., Watanabe, M., Okuda-Ashitaka, E. and Ito, S
  • Organizer: The 40th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: San Diego
• [Presentation] Involvement of prostaglandin F2_α receptor in ATP-induced mechanical allodynia (2009)
  • Author(s): Ito S, Kunori S, Matsumura S, Mabuchi T, Tatsumi S, Sugimoto Y, Minami T
  • Organizer: 北米神経科学会
  • Place of Presentation: アメリカ国シカゴ
  • Year and Date: 2009-10-18
• [Presentation] Involvement of prostaglandin F2α receptor in ATP-induced mechanical allodynia (2009)
  • Author(s): Ito, S., Kunori, S., Mabuchi, T., Tastumi, S., Sugimoto, Y., Matsumura, S. and Minami, T
  • Organizer: The 39th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: Chicago
• [Patent(Industrial Property Rights)] 埋植用鎮痛製剤 (2011)
  • Industrial Property Rights Holder: 荒木吉朗、海堀昌樹、松村伸治、伊藤誠二、權雅憲
  • Filing Date: 2011