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The development of analgesic peptides by Nav channel E3 targeting

Research Project

Project/Area Number 21600021
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 疼痛学
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

INAGAKI Hidetoshi  独立行政法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (90344126)

Research Collaborator TORIBA Michihisa  財団法人日本蛇族学術研究所, 所長 (40109856)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsナトリウムチャネル / E3領域 / ペプチド誘導体 / 組換え体ペプチド / BIAcore / ペプチド系鎮痛剤
Research Abstract

We previously identified ant bioactive peptides from a species of the Japanese ant Strumigenys kumadori, and termed these peptides SKTX1, 2, and 3.In this study, we constructed a cDNA library encoding various SKTX peptide derivatives on T7 phage. From the library, we screened the SKTX derivatives which specifically bind to rNa_v 1.3 channel using the binding ability to E3 domain of the channel. We selected 5 derivatives and the derivatives were recombinantly expressed by E. coli. We confirmed the binding ability of these derivatives by BIA core and the estimated binding ability of the derivatives were micro molar levels.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (15 results)

All 2012 2011 2010 2009

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (9 results)

  • [Journal Article] Functional characterization of Kunitz-type protease inhibitor Pr-mulgins identified from New Guinean Pseudechis australis2012

    • Author(s)
      Inagaki H, Kimoto H, Yamauchi Y, Toriba M, Kubo T
    • Journal Title

      Toxicon

      Volume: 59(1) Issue: 1 Pages: 74-80

    • DOI

      10.1016/j.toxicon.2011.10.005

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification and functional characterization of Kunitz-type toxins from New Guinean Pseudechis australis2011

    • Author(s)
      Inagaki H, Kimoto H, Yamauchi Y, Toriba M, Kubo T
    • Journal Title

      Peptide Science 2010

      Volume: 66

    • NAID

      10028237260

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regional divergence of phospholipase A(2)-like protein cDNAs between New Guinean and Australian Pseudechis australis2010

    • Author(s)
      Inagaki H, Yamauchi Y, Toriba M, Kubo T
    • Journal Title

      Toxicon

      Volume: 56(4) Issue: 4 Pages: 637-9

    • DOI

      10.1016/j.toxicon.2010.04.018

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced activation of the transient receptor potential channel TRPA1 by ajoene, an allicin derivative2010

    • Author(s)
      Yassaka RT, Inagaki H, Fujino T, Nakatani K, Kubo T
    • Journal Title

      Neurosci Res

      Volume: 66(1) Issue: 1 Pages: 99-105

    • DOI

      10.1016/j.neures.2009.09.1712

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regional divergence of phospholipase A(2)-like protein cDNAs between New Guinean and Australian Pseudechis australis.2010

    • Author(s)
      Inagaki H, Yamauchi Y, Toriba M, Kubo T.
    • Journal Title

      Toxicon.

      Volume: 56(4) Pages: 637-639

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced activation of the transient receptor potential channel TRPA1 by ajoene, an allicin derivative.2010

    • Author(s)
      Yassaka RT, Inagaki H, Fujino T, Nakatani K, Kubo T.
    • Journal Title

      Neuroscience research 66(1)

      Pages: 99-105

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Characterization of Phospholipase A2-like proteins, Kunitz-type protease inhibitors, and three finger toxins from Pseudechis rossignolii2011

    • Author(s)
      Inagaki H, Kimoto H, Yamauchi Y, Toriba M, Kubo T.
    • Organizer
      9th AP-IST meeting
    • Place of Presentation
      ロシアウラジオストック市
    • Year and Date
      2011-09-04
    • Related Report
      2011 Final Research Report
  • [Presentation] Characterization of Phospholipase A2-like proteins, Kunitz-type protease inhibitors, and three finger toxins from Pseudechis rossignolii2011

    • Author(s)
      Inagaki H, Kimoto H, Yamauchi Y, Toriba M, Kubo T
    • Organizer
      9th AP-IST meeting
    • Place of Presentation
      ロシアウラジオストック
    • Year and Date
      2011-09-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] Bioactive peptides from venomous animals2011

    • Author(s)
      Inagaki H.
    • Organizer
      International Symposium 2011 for Healthcare
    • Place of Presentation
      韓国、全州市、又石大学
    • Year and Date
      2011-05-13
    • Related Report
      2011 Final Research Report
  • [Presentation] Bioactive peptides from venomous animals2011

    • Author(s)
      Inagaki H
    • Organizer
      International Symposium 2011 for Healthcare
    • Place of Presentation
      韓国全州市又石大学(招待講演)
    • Year and Date
      2011-05-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] Identification and Functional Characterization of Kunitz-type Toxins from New Guinean Pseudechis australis2010

    • Author(s)
      Inagaki H, Kimoto H, Ueno S, Yamauchi Y, Toriba M, and Kubo T.
    • Organizer
      第5回国際ペプチド・シンポジウム
    • Place of Presentation
      京都
    • Year and Date
      2010-12-05
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Novel Bioactive Peptides, SKTX 1, SKTX 2, and SKTX 3, from a Species of the Japanese Ant Genus Strumigenys2010

    • Author(s)
      Inagaki H, Masuko K, Kubo T.
    • Organizer
      第53回日本蟻類研究会
    • Place of Presentation
      館山
    • Year and Date
      2010-08-29
    • Related Report
      2011 Final Research Report
  • [Presentation] パプアピグミーマルガスネーク由来新規生理活性ペプチドp-mulgin 1, 2, 3の同定2010

    • Author(s)
      稲垣英利、山内瑶子、鳥羽通久、久保泰
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-28
    • Related Report
      2011 Final Research Report
  • [Presentation] パプアピグミーマルガスネーク由来新規生理活性ペプチドp-mulgin 1,2,3の同定2010

    • Author(s)
      稲垣英利、山内瑶子、鳥羽通久, 久保泰
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] Ajoene, An Allicin Derivative, Potentiates The Transient Receptor Potential Channel TRPA1-Induced Responses2009

    • Author(s)
      Yassaka RT, Inagaki H, Fujino T, Nakatani K, Kubo T.
    • Organizer
      第36回国際生理学会
    • Place of Presentation
      京都
    • Year and Date
      2009-07-30
    • Related Report
      2011 Final Research Report 2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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