| Project/Area Number |
21602001
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Stem cell biology and medical science
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| Research Institution | Nagaoka University of Technology (2010-2011)
The University of Tokyo (2009) |
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Principal Investigator |
OHNUMA Kiyoshi 長岡技術科学大学, 産学融合トップランナー養成センター, 特任准教授 (50396834)
|
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| Co-Investigator(Kenkyū-buntansha) |
WAKAMOTO Yuichi 東京大学, 大学院・総合文化研究科, 准教授 (30517884)
HEXIG Bayar 東京工業大学, 大学院・生命理工学研究科, 特任助教 (20515168)
MICHIUE Tatsuo 東京大学, 大学院・総合文化研究科, 准教授 (10282724)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 1細胞培養 / ES細胞 / ゆらぎ / 微細加工 / 初期発生 / 一細胞培養 / 揺らぎ / 無血清培養 / E-カドヘリン |
|---|
| Research Abstract |
The mechanism that provides stability during embryonic development remains to be uncovered. To do this, we made a system to observe a single-cell reaction to culture medium stimulation. The system consists of four parts : ES cells, which are a good model for early development, a culture medium without any unknown factors, a specially coated culture dish that enabled us to dissociate cells without aggregation, and time-lapse microscopy. This system enabled us to monitor life-and-death, proliferation, and differentiation of a single ES cell. Single-cell level observation of stimulation response of ES cells will deepen our understanding of developmental stability.
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