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Development of PET imaging system to visualize adult neurogenesis

Research Project

Project/Area Number 21602003
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Stem cell biology and medical science
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

UEKI Takatoshi  浜松医科大学, 医学部, 准教授 (60317328)

Co-Investigator(Kenkyū-buntansha) FURUKAWA Hiromu  浜松医科大学, 医学部, 助教 (20209167)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords神経幹細胞 / 神経新生
Research Abstract

An accumulating body of evidence point to the involvement of dysfunction of adult neurogenesis in the pathophysiology of neurological and psychiatric disorders such like Parkinson's disease, major depression and schizophrenia. Recently neural stem cell is recognized as a therapeutic target of diagnosis and treatment for those diseases. In the present study, therefore, at first animal system to visualize and evaluate adult neurogenesis was established, where lentivirally infected neural stem cell expresses exogenously neutral amino acid transporter LAT4 and uptake PET tracer[ 18F] FMT. In the adult rat brain transplanted or endogenous neural stem cells were infected with lentivirus and their behavioral dynamics was analyzed in vivo under PET. In addition the effects of drug candidate chemical compounds, which act as a mitogen in vitro for neural stem cell, on adult neurogenesis were quantitatively evaluated by PET. At second, in this study the established imaging technology was applied to disease model mice of major depression or schizophrenia, in which neural stem cells in the SVZ and SGZ were infected with lentivirus to express LAT4, and subsequently the mice were subjected to in vivo analysis of motility of neural stem cells in the diseased brain and study of the onset process of adult neurogenesis dysfunction. Antidepressant or psychoactive drug were administered to those animals, and the involvement of neural stem cell in the adult brain in the pathophysiology of psychiatric disorders was investigated.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (10 results)

All 2011 2010 2009

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (5 results)

  • [Journal Article] Elevated transcription factor specificity protein 1 in autistic brains alters the expression of autism candidate genes2011

    • Author(s)
      Thanseem I, Anitha A, Nakamura K, Suda S, Iwata K, Matsuzaki H, Ohtsubo M, Ueki T, Katayama T, Iwata Y, Suzuki K, Minoshima S, Mori N
    • Journal Title

      Biol Psychiatry

      Volume: 71 Pages: 410-418

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of brain amyloid deposition and reduced glucose metabolism on the default mode of brain function in normal aging2011

    • Author(s)
      Kikuchi M, Hirosawa T, Yokokura M, Yagi S, Mori N, Yoshikawa E, Yoshihara Y, Sugihara G, Takebayashi K, Iwata Y, Suzuki K, Nakamura K, Ueki T, Minabe Y, Ouchi Y
    • Journal Title

      J Neurosci

      Volume: 31 Pages: 11193-11199

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The identification of novel protein, brain-derived integrating factor-1(BDIF1), which interacts with astrocytic gap junctional protein2011

    • Author(s)
      Ito T, Ueki T, Furukawa H, Sato K
    • Journal Title

      Neurosci Res

      Volume: 70 Pages: 330-333

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] In vivo changes in microglial activation and amyloid deposits in brain regions with hypometabolism in Alzheimer's disease2011

    • Author(s)
      Yokokura M, Mori N, Yagi S, Yoshikawa E, Kikuchi M, Yoshihara Y, Wakuda T, Sugihara G, Takebayashi K, Suda S, Iwata Y, Ueki T, Tsuchiya KJ, Suzuki K, Nakamura K, Ouchi Y
    • Journal Title

      Eur J Nucl Med Mol Imaging

      Volume: 38 Pages: 343-351

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Altered brain serotonin transporter and associated glucose metabolism in Alzheimer disease2009

    • Author(s)
      Ouchi Y, Yoshikawa E, Futatsubashi M, Yagi S, Ueki T, Nakamura K
    • Journal Title

      J Nucl Med

      Volume: 50 Pages: 1260-1266

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Presentation] NG2細胞におけるCD44プロセシングの画像化技術の創出と、その多発性硬化症の幹細胞療法への応用2011

    • Author(s)
      植木孝俊
    • Organizer
      第16回グリア研究会
    • Place of Presentation
      名古屋
    • Year and Date
      2011-10-22
    • Related Report
      2011 Final Research Report
  • [Presentation] NG2細胞におけるCD44プロセシングの画像化技術の創出と、その多発性硬化症のための幹細胞療法への応用2011

    • Author(s)
      植木孝俊
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      横浜
    • Year and Date
      2011-09-15
    • Related Report
      2011 Final Research Report
  • [Presentation] Development of molecular imaging system to visualize the processing of CD44 in NG2 cell, and its contribution to understanding of the role of CD44 in the CNS2010

    • Author(s)
      Takatoshi Ueki
    • Organizer
      The 29th Naito conference on glia world
    • Place of Presentation
      Hayama
    • Year and Date
      2010-10-06
    • Related Report
      2011 Final Research Report
  • [Presentation] Development of functional molecular probe and its application for diagnosis of Alzheimer's disease2010

    • Author(s)
      植木孝俊
    • Organizer
      第53回日本神経化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-09-03
    • Related Report
      2011 Final Research Report
  • [Presentation] γセクレターゼ酵素活性依存性分子機能プローブの創出と、そのアルツハイマー病診断への応用2010

    • Author(s)
      植木孝俊
    • Organizer
      第33回神経科学大会
    • Place of Presentation
      神戸コンベンションセンター(兵庫県)
    • Year and Date
      2010-09-03
    • Related Report
      2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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