• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of Hematopoietic stem cell regulation through Polycomb-group complex 1

Research Project

Project/Area Number 21602004
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Stem cell biology and medical science
Research InstitutionBeppu University (2011)
Hiroshima University (2009-2010)

Principal Investigator

OHTSUBO Motoaki  別府大学, 食物栄養科学部, 教授 (10211799)

Co-Investigator(Kenkyū-buntansha) TAKIHARA Yoshihiro  広島大学, 原爆放射線医科学研究所, 教授 (60226967)
YASUNAGA Shin ichiro  広島大学, 原爆放射線医科学研究所, 准教授 (50336111)
OHNO Yoshinori  広島大学, 原爆放射線医科学研究所, 助教 (10548986)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords再生医学 / 発生・分化 / 造血幹細胞 / ポリコーム複合体 / Geminin / DNA複製制御 / DMA複製制御
Research Abstract

We revealed that Polycomb-group(PcG) complex 1 regulates the protein's stability of Geminin either by directly acting as the E3 ubiquitin ligase or indirectly through transcriptional regulation of Hoxa9 and Hoxb4.Our observations will contribute to the development of a method to enhance hematopoietic stem cell activity by manipulating the E3 ubiquitin ligase activity of PcG complex 1 through the modification of Scmh1.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (20 results)

All 2012 2011 2010 2009 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (8 results) Book (2 results) Remarks (4 results)

  • [Journal Article] Overexpression of BMI-1 correlates with drug resistance in B-cell lymphoma cells through the stabilization of surviving expression2012

    • Author(s)
      Bhattacharyya, J., Mihara, K., Ohtsubo, M., Yasunaga, S., Takei, Y., Yanagihara, K., Sakai, A., Hishi, M., Takihara, Y., Kimura, A.
    • Journal Title

      Cancer Science

      Volume: 103(1) Pages: 34-41

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Decreased expression in nuclear factor-kB essential modulator due to a novel splice-site mutation causes X-linked ectodermal dysplasia with immunodeficiency2011

    • Author(s)
      Karakawa, S., Okada, S., Tsumura, M., Mizoguchi, Y., Ohno, N., Yasunaga, S., Ohtsubo, M., Kawai, T., Nishikomori, R., Sakaguchi, T., Takihara, Y., Kobayashi, M.
    • Journal Title

      Journal of Clinical Immunology

      Volume: 31(5) Pages: 762-772

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular pathogenesis of a novel mutation, G108D, in short-chain acyl-CoA dehydrogenase identified in subjects with short-chain acyl-CoA dehydrogenase deficiency2010

    • Author(s)
      Shirao, K., Okada, S., Tajima, G., Tsumura, M., Hara, K., Yasunaga, S., Ohtsubo, M., Hata, I., Sakura, N., Shigematsu, Y., Takihara, Y., Kobayashi, M.
    • Journal Title

      Human Genetics

      Volume: 127(6) Pages: 619-628

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hoxb4 transduction down-regulates Geminin protein, providing hematopoietic stem and progenitor cells with proliferation potential2010

    • Author(s)
      Ohno, Y., Yasunaga, S., Ohtsubo, M., Mori, S., Tsumura, M., Okada, S., Ohta, T., Ohtani, K., Kobayashi, M., Takihara, Y.
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 107 Pages: 21529-21534

    • NAID

      40019027599

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hoxb4 transduction down-regulates Geminin protein, providing hematopoietic stem and progenitor cells with proliferation potential.2010

    • Author(s)
      Ohno, Y., Yasunaga, S., Ohtsubo, M., Mori, S., Tsumura, M., Okada, S., Ohta, T., Ohtani, K., Kobayashi, M., Takihara, Y.
    • Journal Title

      Proc.Natl.Acad.Sci.USA

      Volume: 107 Pages: 21529-21534

    • NAID

      40019027599

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular pathogenesis of a novel mutation, G108D, in short-chain acyl-CoA dehydrogenase identified in subjects with short-chain acyl-CoA dehydrogenase deficiency.2010

    • Author(s)
      Shirao, K., Okada, S., Tajima, G., Tsumura, M., Hara, K., Yasunaga, S., Ohtsubo, M., Hata, I., Sakura, N., Shigematsu, Y., Takihara, Y., Kobayashi, M.
    • Journal Title

      Hum.Genet.

      Volume: 127(6) Pages: 619-628

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Role for an Scmh1-mediated molecular network governing Geminin expression in hematopoiesis2012

    • Author(s)
      Ohtsubo, M., Yasunaga, Y., Ohno, Y., Kawashima, A., Tetsuguchi, H., Saeki, K., Nakashima, Y., Takihara, Y.
    • Organizer
      International Symposium 50^<th> Anniversary of RIRBM, Hiroshima University
    • Place of Presentation
      広島国際会議場
    • Year and Date
      2012-02-21
    • Related Report
      2011 Final Research Report
  • [Presentation] Hole for an Scmh1-mediated molecular network governing Geminin expression in hematopoiesis2012

    • Author(s)
      Ohtsubo, M., Yasunaga, Y., Ohno, Y., Kawashima, A., Tetsuguchi, H., Saeki, K, Nakashima, Y., Takihara, Y.
    • Organizer
      International Symposium 50^<th> Anniversary of RIRBM, Hiroshima University
    • Place of Presentation
      広島国際会議場
    • Year and Date
      2012-02-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] A new strategy for maintaining HSC activity through the modulation of Geminin expression2011

    • Author(s)
      Yasunaga, Y., Ohno, Y., Ohtsubo, M., Furutani, T., Kawashima, A., Tetsuguchi, H., Saeki, K., Nakashima, Y., Takihara, Y.
    • Organizer
      日本血液学会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2011-10-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] Study of a 5-FU-induced stabilization signal for Scmh1, a component of Polycomb-group complex 1 E3 ligase2009

    • Author(s)
      Ohtsubo, M., Yasunaga, S., Ohno, Y., Tsumura, M., Kageyama, Y., Mori, S., Takihara, Y.
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-10
    • Related Report
      2011 Final Research Report
  • [Presentation] Study of a 5-FU-induced stabilization signal for Scmhl, a component of Polycomb-group complex 1 E3 ligase2009

    • Author(s)
      大坪素秋
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] HSCの活性維持に必須なポリコーム複合体1の構成因子Scmh1の機能ドメインの解析2009

    • Author(s)
      大坪素秋
    • Organizer
      第71回日本血液学会学術集会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2009-10-23
    • Related Report
      2011 Final Research Report
  • [Presentation] HSCの活性維持に必須なポリコーム複合体1の構成因子 Scmhl の機能ドメインの解析2009

    • Author(s)
      大坪素秋
    • Organizer
      第71回日本血液学会学術集会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2009-10-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] Role of Scmh1 in the regulation of Polycomb-group complex 1, which acts as the E3 ubiquitin ligase for histone H2A and Geminin2009

    • Author(s)
      Ohtsubo, M., Yasunaga, S., Tsumura, M., Ohno, Y., Kageyama, Y., Kimura, S., Tokimoto, R., Takihara, Y.
    • Organizer
      The 7th Stem Cell Research Symposium
    • Place of Presentation
      Tokyo
    • Year and Date
      2009-05-16
    • Related Report
      2011 Final Research Report
  • [Book] 血液フロンティア・がん幹細胞研究の新たな展開・幹細胞における細胞周期制御2012

    • Author(s)
      瀧原義宏,安永晋一郎,大野芳典,大坪素秋
    • Publisher
      医薬ジャーナル社
    • Related Report
      2011 Final Research Report
  • [Book] 血液フロンティア・がん幹細胞研究の新たな展開・幹細胞における細胞周期制御2012

    • Author(s)
      瀧原義宏、安永晋一郎、大野芳典、大坪素秋
    • Total Pages
      11
    • Publisher
      医薬ジャーナル社
    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://home.hiroshima-u.ac.jp/dscb/index.html

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://home.hiroshima-u.ac.jp/dscb/index.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://home.hiroshima-u.ac.jp/dscb/index.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://home.hiroshima-u.ac.jp/dscb/index.html

    • Related Report
      2009 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi