Epigenetic regulation of embryonic and somatic stem cells by Polycomb

• Project/Area Number: 21602006
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Stem cell biology and medical science
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: ENDOH Mitsuhiro 独立行政法人理化学研究所, 免疫器官形成研究グループ, 研究員 (40391883)
• Co-Investigator(Renkei-kenkyūsha): KOSEKI Haruhiko 独立行政法人理化学研究所, 免疫器官形成研究グループ, グループリーダー (40225446)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ES細胞 / 幹細胞 / エピジェネティクス / クロマチン / 転写 / 発生 / 分化 / 胚性幹細胞 / ポリコーム / ヒストン修飾 / 初期発生 / 細胞分化 / ユビキチン化ヒストンH2A / 転写抑制 / 転写制御

Research Abstract

Analysis using Ring1A/B conditional knockout embryonic stem(ES) cells revealed that Ring1A/B contribute to maintain ES cell identity by repressing developmental regulators. We also reported that Ring1A/B-mediated mono-ubiquitination of histone H2A is an essential step for transcriptional repression of the target genes. Furthermore, we found that histone ubiquitination activity of Ring1A/B is also required for the maintenance of epiblast stem cells derived from a 6.5 dpc embryo.

Research Products

• [Journal Article] Bernstein BE and Koseki H Histone H2A mono-ubiquitination is a crucial step to mediate PRC1 dependent repression of developmental genes to maintain ES cell identity (2012)
  • Author(s): Endoh M, Endo TA, Endoh T, Isono K, Sharif J, Ohara O, Toyoda T, Ito T, Eskeland R, Bickmore WA, Vidal M
  • Journal Title: PLoS Genet Volume: (in press)
  • Peer Reviewed
• [Journal Article] RYBP represses endogenous retroviruses, preimplantation-and germline-specific genes in mouse embryonic stem cells (2012)
  • Author(s): Hisada K, Sanchez C, Endo T, Endoh M, Roman-Trufero M, Sharif J, Koseki H, Vidal M
  • Journal Title: Mol Cell Biol Volume: 32 Pages: 1139-1149
  • Peer Reviewed
• [Journal Article] RYBP Represses Endogenous Retroviruses and Preimplantation- and Germ Line-Specific Genes in Mouse Embryonic Stem Cells (2012)
  • Author(s): Hisada K
  • Journal Title: Mol Cell Biol Volume: 32 Issue: 6 Pages: 1139-1149
  • DOI: 10.1128/mcb.06441-11
  • Peer Reviewed
• [Journal Article] The Hbo1-Brd1/Brpf2 complex is responsible for global acetylation of H3K14 and required for fetal liver erythropoiesis (2011)
  • Author(s): Mishima Y, Miyagi S, Saraya A, Negishi M, Endoh M, Endo TA, Toyoda T, Shinga J, Katsumoto T, Chiba T, Yamaguchi N, Kitabayashi I, Koseki H, Iwama A
  • Journal Title: Blood Volume: 118 Pages: 2443-2453
  • Peer Reviewed
• [Journal Article] Genome-wide analysis of target genes regulated by HoxB4 in hematopoietic stem and progenitor cells developing from embryonic stem cells (2011)
  • Author(s): Oshima M, Endoh M, Endo TA, Toyoda T, Nakajima-Takagi Y, Sugiyama F, Koseki H, Kyba M, Iwama A, Osawa M
  • Journal Title: Blood Volume: 117
  • Peer Reviewed
• [Journal Article] Mammalian polycomb-like Pcl2/Mtf2 is a novel regulatory component of PRC2 that can differentially modulate polycomb activity both at the Hox gene cluster and at Cdkn2a genes (2011)
  • Author(s): Li X, Isono K, Yamada D, Endo TA, Endoh M, Shinga J, Mizutani-Koseki Y, Otte AP, Casanova M, Kitamura H, Kamijo T, Sharif J, Ohara O, Toyada T, Bernstein BE, Brockdorff N, Koseki H
  • Journal Title: Mol Cell Biol Volume: 31 Pages: 351-364
  • Peer Reviewed
• [Journal Article] Epigenetic memory meets G2/M : to remember or to forget (2011)
  • Author(s): Sharif J, Endoh M, Koseki H
  • Journal Title: Dev Cell Volume: 20 Pages: 5-6
• [Journal Article] Genome-wide analysis of target genes regulated by HoxB4 in hematopoietic stem and progenitor cells developing from embryonic stem cells (2011)
  • Author(s): Oshima
  • Journal Title: Blood Volume: 117 Issue: 15 Pages: e142-e150
  • DOI: 10.1182/blood-2010-12-323212
  • Peer Reviewed
• [Journal Article] Epigenetic memory meets G2/M : to remember or to forget? (2011)
  • Author(s): Sharif J
  • Journal Title: Dev Cell Volume: 20 Pages: 5-6
• [Journal Article] Mammalian Polycomb-Like Pcl2/Mtf2 Is a Novel Regulatory Component of PRC2 That Can Differentially Modulate Polycomb Activity both at the Hox Gene Cluster and at Cdkn2a Genes (2011)
  • Author(s): Xiangzhi Li
  • Journal Title: Molecular and Cellular Biology Volume: 31 Pages: 351-364
  • Peer Reviewed
• [Journal Article] Genome-wide analysis of target genes regulated by HoxB4 in hematopoietic stem and progenitor cells developing from embryonic stem cells (2011)
  • Author(s): Motohiko Oshima
  • Journal Title: Blood Volume: (In press)
  • Peer Reviewed
• [Journal Article] Epigenetic Memory Meets G2/M : To Remember or To Forget? (2011)
  • Author(s): Jafar Sharif
  • Journal Title: Developmental Cell Volume: 20 Pages: 5-6
• [Journal Article] ヒストンH2Aのユビキチン化による遺伝子発現の制御 (2009)
  • Author(s): 遠藤充浩、古関明彦
  • Journal Title: 生体の科学 Volume: 60巻 Pages: 533-540
• [Journal Article] ヒストンH2Aのユビキチン化による遺伝子発現の制御 (2009)
  • Author(s): 遠藤充浩
  • Journal Title: 生体の科学 60 Pages: 533-540
• [Presentation] ポリコーム群Ring1A/BによるヒストンH2Aユビキチン化のES細胞における局在と役割 (2012)
  • Author(s): 遠藤充浩、遠藤高帆、磯野協一、古関明彦
  • Organizer: 第6回エピジェネティクス研究会
  • Place of Presentation: 東京一ツ橋学術総合センター
• [Presentation] H2A ubiquitination is an essential step for Polycomb repression of differentiation genes in ES cells (2011)
  • Author(s): 遠藤充浩
  • Organizer: 日本エピジェネティクス研究会第5回年会
  • Place of Presentation: KKRホテル熊本(熊本市)
  • Year and Date: 2011-05-19
• [Presentation] H2A ubiquitination is an essential step to mediate PRC1 Polycomb silencing of differentiation genes in ES cells (2011)
  • Author(s): Endoh M and Koseki H
  • Organizer: 第9回幹細胞シンポジウム
  • Place of Presentation: 泉ガーデンギャラリー、東京
  • Year and Date: 2011-05-14
• [Presentation] H2A ubiquitination is an essential step to mediate PRC1 Polycomb silencing of differentiation genes in ES cells (2011)
  • Author(s): 遠藤充浩
  • Organizer: 第9回幹細胞シンポジウム
  • Place of Presentation: 泉ガーデンギャラリー(港区)
  • Year and Date: 2011-05-13
• [Presentation] ユビキチン化ヒストンH2AのES細胞における局在と役割 (2011)
  • Author(s): 遠藤充浩、遠藤高帆、磯野協一、古関明彦
  • Organizer: 第5回エピジェネティクス研究会
  • Place of Presentation: KKRホテル熊本
• [Presentation] クロマチン制御因子ポリコーム群による哺乳類初期胚における幹細胞制御 (2010)
  • Author(s): 遠藤充浩
  • Organizer: 京大放生研セミナー
  • Place of Presentation: 京都大学放射線生物研究センター
  • Year and Date: 2010-06-04
• [Presentation] クロマチン制御因子ポリコーム群による哺乳類初期胚における幹細胞制御 (2010)
  • Author(s): 遠藤充浩
  • Organizer: 京都大学放射線生物学研究所セミナー
  • Place of Presentation: 京都大学放射線生物学研究所、京都市
  • Year and Date: 2010-06-04
• [Presentation] ポリコーム群は転写因子の抑制を介して栄養芽幹細胞の分化を促進する (2010)
  • Author(s): 遠藤充浩、遠藤高帆、古関明彦
  • Organizer: 第43回日本発生生物学会
  • Place of Presentation: 京都国際会議場
• [Presentation] ポリコーム群とコアな転写因子のクロストークによる栄養芽幹細胞の分化制御 (2010)
  • Author(s): 遠藤充浩、遠藤高帆、古関明彦
  • Organizer: 第4回日本エピジェネティクス研究会
  • Place of Presentation: 米子市文化ホール
• [Presentation] Polycomb temporally regulates trophoblast development by promoting stem cell differentiation (2010)
  • Author(s): Endoh M, Endo TA, and Koseki H
  • Organizer: 第8回幹細胞シンポジウム
  • Place of Presentation: 淡路夢舞台国際会議場
• [Presentation] Polycomb temporally regulates trophoblast development by promoting stem cell differentiation (2010)
  • Author(s): 遠藤充浩
  • Organizer: 第8回幹細胞シンポジウム
  • Place of Presentation: 淡路夢舞台国際会議場、淡路市
• [Presentation] ポリコーム群とコアな転写因子のクロストークによる栄養芽幹細胞の分化制御 (2010)
  • Author(s): 遠藤充浩
  • Organizer: 第4回エピジェネティクス研究会
  • Place of Presentation: 米子文化ホール、米子市
• [Presentation] ポリコーム群は転写因子の抑制を介して栄養芽幹細胞の分化を促進する (2010)
  • Author(s): 遠藤充浩
  • Organizer: 第43回日本発生生物学会
  • Place of Presentation: 京都国際会議場、京都市
• [Presentation] PRC2-dependent and -independent deposition of Ring1B-mediated uH2A at developmental gene promoters in ES cells (2010)
  • Author(s): 遠藤充浩
  • Organizer: Cold Spring Harbor Laboratory Meeting, Mouse Development, Genetics & Genomics.
  • Place of Presentation: Cold Spring Harbor Laboratory, New York
• [Presentation] PRC1 suppress ES cell differentiation programs through PRC2-dependent and PRC2-independent mechanisms (2009)
  • Author(s): 遠藤充浩
  • Organizer: Cold Spring Harbor Laboratory(CSHL)Meeting, Mouse Genetics & Genomics : Development & Disease
  • Place of Presentation: Sanger institute(イギリス、ケンブリッジ)
  • Year and Date: 2009-09-03
• [Presentation] ポリコーム群によるヒストン修飾を介したES細胞の未分化性維持 (2009)
  • Author(s): 遠藤充浩
  • Organizer: 第7回幹細胞シンポジウム
  • Place of Presentation: 泉ガーデンセンター(東京港区)
  • Year and Date: 2009-05-16
• [Presentation] ポリコーム群Ring1A/Bによるヒストン修飾を介した幹細胞制御 (2009)
  • Author(s): 遠藤充浩、遠藤高帆、古関明彦
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際会議場
• [Presentation] PRC1 suppresses ES cell differentiation programs through PRC2-dependent and PRC2-independent mechanisms (2009)
  • Author(s): 遠藤充浩、遠藤高帆、古関明彦
  • Organizer: 第19回日本数理生物学会年会
  • Place of Presentation: 東京大学駒場キャンパス
• [Presentation] PRC1 suppresses ES cell differentiation programs through PRC2-dependent and PRC2-independent mechanisms (2009)
  • Author(s): Endoh M, Endo TA, Endoh T, Vidal M, and Koseki H
  • Organizer: Cold Spring Harbor Laboratory Meeting
  • Place of Presentation: Cambridge, UK
• [Presentation] Roles of Polycomb group proteins Ring1A/B in mouse ES cells and TS cells (2009)
  • Author(s): 遠藤充浩、古関明彦
  • Organizer: 第7回幹細胞シンポジウム
  • Place of Presentation: 学術総合センター
• [Presentation] ポリコーム群Ring1A/Bによるヒストン修飾を介した幹細胞制御 (2009)
  • Author(s): 遠藤充浩、遠藤高帆、古関明彦
  • Organizer: 第3回日本エピジェネティクス研究会年会
  • Place of Presentation: 東京一ツ橋学術総合センター
• [Book] ヒストンH2Aのユビキチン化による遺伝子発現の制御生体の科学60巻 (2009)
  • Author(s): 遠藤充浩、古関明彦