| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Research Abstract |
Okadaic acid binding proteins OABP_s 2. 1 and 2. 3, originally isolated from the sponge Halichondria okadai, were stably expressed as recombinant proteins in E. coli. Dissociation constants of OA from recombinant OABP_s 2. 1 and 2. 3 were determined in the binding assays. The okadaic acid binding proteins were deduced to be the sponge proteins but not those from symbiotic species. Furthermore, we quantitated okadaic acid and OABP2. 1 in the sponge collected from various places in Japan, and speculated a possible self-defense system of the sponge.
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