Development of specific gene activation method using polyamide-transcription factor conjugates
| Project/Area Number |
21603008
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemical biology
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| Research Institution | Kyoto University |
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Principal Investigator |
ENDO Masayuki 京都大学, 物質細胞統合システム拠点, 准教授 (70335389)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | エピジェネティック制御 / DNAナノ構造 / ヌクレオソーム / 原子間力顕微鏡 / ポリアミド / ヒストン / DNA修復酵素 / 1分子イメージング |
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| Research Abstract |
We tried to investigate the regulation of gene expression using synthetic pyrrole-imidazole polyamide. For the investigation of the mechanism of epigenetic regulation, we developed the imaging method for nucleosome and related enzymes. For direct visualization of the nucleosome, we constructed the single molecule observation system using the designed DNA nanostructures. We successfully visualized the sequence-selectivity of functionalized polyamide in the designed DNA nanostructure. We also constructed the single-molecule observation system for the behavior of enzymes using high-speed atomic force microscopy. Using this system, we successfully captured the nucleosome to the DNA nanoscaffold, and observed its behavior at the single-molecule level.
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Report
(4 results)
Research Products
(69 results)
