| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
We tried to construct high-power biofuel cells. The optimal orientation of enzymes on the electrode surface, three-dimensional immobilization of enzymes, and immobilization of plural enzymes on an electrode were investigated. We found the best treatment of the electrode for enzyme immobilization. Using a novel thermophilic alcohol dehydrogenase, a high current density was observed at 80℃more than 10 times at room temperature. An extremely high surface coverage of electrostatically active protein, 270 proteins in a fully packed monolayer, was obtained through use of the gold nanoparticle-modified electrode. We also found a novel alcohol dehydrogenase which can catalyze 4-electron oxidation of ethanol.
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