Microtomographic analysis of biological tissues

• Project/Area Number: 21611009
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Medical Physics/Radiological Technology
• Research Institution: Tokai University
• Principal Investigator: MIZUTANI Ryuta 東海大学, 工学部, 教授 (70272482)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: 生体組織 / 三次元構造 / マイクロトモグラフィ / 神経回路 / マイクロＣＴ / マイクロトモグラフィー / マイクロCT

Research Abstract

Computed tomography (CT) is a noninvasive technique for visualizing three-dimensional structures. Tomographic slices are reconstructed from a series of projection images acquired by a rotational scan. The application of x-ray microscopic approaches to CT analysis has led to the visualization of the three-dimensional microstructures of biological samples. We determined three-dimensional cellular and subcellular structure of human cerebral cortex stained with high atomic-number probes. The obtained structure indicated that the neuronal networks and capillary vessel architectures were visualized as the three-dimensional distribution of x-ray absorption coefficients. The capillary microstructures were used as a template to fabricate a resin model of capillary blood vessels. These results indicated that x-ray microtomography is a potential method of visualizing human tissue structures.

Research Products

• [Journal Article] microtomographic visualization of Escherichia coli by metalloprotein overexpression. (2013)
  • Author(s): Ryuta Mizutani, Keisuke Taguchi, Masato Ohtsuka, Minoru Kimura, Akihisa Takeuchi, Kentaro Uesugi,Yoshio Suzuki
  • Journal Title: J. Synchrotron Radiation Volume: (in press)
  • Peer Reviewed
• [Journal Article] Structure of a double-stranded DNA (6-4) photoproduct in complex with the 64M-5 antibody Fab (2013)
  • Author(s): Hideshi Yokoyama, Ryuta Mizutani, Yoshinori Satow
  • Journal Title: Acta Crystallographica Volume: D69 Pages: 504-512
  • Peer Reviewed
• [Journal Article] Crystal structure of 6-guanidinohexanoyl trypsin near the optimum pH reveals the acyl-enzyme intermediate to be deacylated (2013)
  • Author(s): Yosuke Masuda, Yasushi Nitanai, Ryuta Mizutani, Shuji Noguchi
  • Journal Title: Proteins Volume: 81 Pages: 526-530
  • Peer Reviewed
• [Journal Article] Structure of the DNA (6-4) photoproduct dTT(6-4)TT in complex with the 64M-2 antibody Fab fragment implies increased antibody-binding affinity by the flanking nucleotides (2013)
  • Author(s): Hideshi Yokoyama, Ryuta Mizutani, Yoshinori Satow, Kousuke Sato, YasuoKomatsu, Eiko Ohtsuka, Osamu Nikaido
  • Journal Title: Acta Crystallographica Volume: D68 Pages: 232-238
  • Peer Reviewed
• [Journal Article] X-ray microtomographic visualization of Escherichia coli by metalloprotein overexpression. (2013)
  • Author(s): Ryuta Mizutani, Keisuke Taguchi, Masato Ohtsuka, Minoru Kimura, Akihisa Takeuchi, Kentaro Uesugi, Yoshio Suzuki
  • Journal Title: J. Synchrotron Radiation Volume: 20
  • Peer Reviewed
• [Journal Article] Structure of a double-stranded DNA (6–4) photoproduct in complex with the 64M-5 antibody Fab. (2013)
  • Author(s): Hideshi Yokoyama, Ryuta Mizutani, Yoshinori Satow
  • Journal Title: Acta Crystallographica D Volume: 69 Issue: 4 Pages: 504-512
  • DOI: 10.1107/s0907444912050007
  • Peer Reviewed
• [Journal Article] Crystal structure of 6-guanidinohexanoyl trypsin near the optimum pH reveals the acyl-enzyme intermediate to be deacylated. (2013)
  • Author(s): Yosuke Masuda, Yasushi Nitanai, Ryuta Mizutani, Shuji Noguchi
  • Journal Title: Proteins Volume: 81 Issue: 3 Pages: 526-530
  • DOI: 10.1002/prot.24206
  • Peer Reviewed
• [Journal Article] X-ray microtomography in biology (2012)
  • Author(s): Ryuta Mizutani, Yoshio Suzuki
  • Journal Title: Micron Volume: 43(2-3) Pages: 104-115
  • Peer Reviewed
• [Journal Article] Structure of the DNA (6-4) photoproduct dTT(6-4)TT in complex with the 64M-2 antibody Fab fragment implies increased antibody-binding affinity by the flanking nucleotides (2012)
  • Author(s): Hideshi Yokoyama
  • Journal Title: Acta Crystallogr.D.Biol.Crystallogr. Volume: 68 Issue: 3 Pages: 232-238
  • DOI: 10.1107/s0907444912000327
  • Peer Reviewed
• [Journal Article] Building human brain network in 3D coefficient map determined by X-ray microtomography (2011)
  • Author(s): Ryuta Mizutani, Akihisa Takeuchi, Kentaro Uesugi, Susumu Takekoshi, Naoya Nakamura, Yoshio Suzuki
  • Journal Title: AIP Conf. Proc Volume: 1365 Pages: 403-406
  • Peer Reviewed
• [Journal Article] Building human brain network in 3D coefficient map determined by X-ray microtomography (2011)
  • Author(s): Ryuta Mizutani, Akihisa Takeuchi, Kentaro Uesugi, Susumu Takekoshi, Naoya Nakamura, Yoshio Suzuki
  • Journal Title: AIP Conference Proceedings Volume: 1365 Pages: 403-406
  • DOI: 10.1063/1.3625388
  • Peer Reviewed
• [Journal Article] Building human brain network in 3D coefficient map determined by X-ray microtomography (2011)
  • Author(s): Ryuta Mizutani, Akihisa Takeuchi, Kentaro Uesugi, Susumu Takekoshi, Naoya Nakamura, Yoshio Suzuki
  • Journal Title: AIP Conf.Ser Volume: (in press)
  • Peer Reviewed
• [Journal Article] Microtomographic analysis of neuronal circuits of human brain (2010)
  • Author(s): Ryuta Mizutani, Akihisa Takeuchi, Kentaro Uesugi, Susumu Takekoshi, R. Yoshiyuki Osamura, Yoshio Suzuki
  • Journal Title: Cerebral Cortex Volume: 20(7) Pages: 1739-1748
  • Peer Reviewed
• [Journal Article] Estimation of presampling modulation transfer function in synchrotron radiation microtomography (2010)
  • Author(s): Ryuta Mizutani, Keisuke Taguchi, Akihisa Takeuchi, Kentaro Uesugi, Yoshio Suzuki
  • Journal Title: Nuclear Inst. and Methods in Physics Research A Volume: 621(1-3) Pages: 615-619
  • Peer Reviewed
• [Journal Article] Submicrometer tomographic resolution examined using a micro-fabricated test object (2010)
  • Author(s): Ryuta Mizutani, Akihisa Takeuchi,R. Yoshiyuki Osamura, Susumu Takekoshi, Kentaro Uesugi, Yoshio Suzuki
  • Journal Title: Micron Volume: 41(1) Pages: 90-95
  • Peer Reviewed
• [Journal Article] Estimation of presampling modulation transfer function in synchrotron radiation microtomography (2010)
  • Author(s): Ryuta Mizutani, Keisuke Taguchi, Akihisa Takeuchi, Kentaro Uesugi, Yoshio Suzuki
  • Journal Title: Nuclear Inst.and Methods in Physics Research A Volume: 621 Pages: 615-619
  • Peer Reviewed
• [Journal Article] Submicrometer tomographic resolution examined using a micro-fabricated test object (2010)
  • Author(s): R.Mizutani, A.Takeuchi, R.Y.Osamura, S.Takekoshi, K.Uesugi, Y.Suzuki
  • Journal Title: Micron 41 Pages: 90-95
  • Peer Reviewed
• [Journal Article] Microtomographic analysis of neuronal circuits of human brain (2010)
  • Author(s): R.Mizutani, A.Takeuchi, K.Uesugi, S.Takekoshi, R.Y.Osamura, Y.Suzuki
  • Journal Title: Cerebral Cortex (in press)
  • Peer Reviewed
• [Journal Article] Three-dimensional cellular and subcellular structures of human brain tissue determined by microtomography (2009)
  • Author(s): Ryuta Mizutani, Akihisa Takeuchi, Susumu Takekoshi, R. Yoshiyuki Osamura, Kentaro Uesugi, Yoshio Suzuki
  • Journal Title: Journal of Physics: Conference Series Volume: 186 Pages: 12108-12108
  • Peer Reviewed
• [Journal Article] Three-dimensional cellular and subcellular structures of human brain tissue determined by microtomography (2009)
  • Author(s): R.Mizutani, A.Takeuchi, S.Takekoshi, R.Y.Osamura, K.Uesugi, Y.Suzuki
  • Journal Title: Journal of Physics : Conf. Ser. 186 Pages: 12108-12108
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): R.Mizutani, A.Takeuchi, K.Uesugi, S.Takekoshi, R.Y.Osamura, Y.Suzuki
  • Journal Title: Three-dimensional microstructural analysis of human brain tissue by using synchrotron radiation microtomographs. In Handbook on White Matter(Nova Science Publishers)(eds. Westland, T.B. & Calton, R.N.) Pages: 247-277
• [Presentation] X線マイクロトモグラフィ法によるヒト大脳皮質の三次元構造解析 (2012)
  • Author(s): 雑賀里乃,伊藤安希,竹腰進,中村直哉,新井誠,大島健一,糸川昌成,竹内晃久,上杉健太朗,鈴木芳生,水谷隆太
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-12-15
• [Presentation] X線マイクロトモグラフィ法によるヒト大脳皮質の三次元構造解析 (2012)
  • Author(s): 水谷隆太
  • Organizer: SPring-8シンポジウム2012
  • Place of Presentation: 大阪大学コンベンションセンター
  • Year and Date: 2012-08-25
• [Presentation] Ｘ線マイクロトモグラフィ法によるヒト大脳皮質の三次元構造解析 (2012)
  • Author(s): 1. 雑賀里乃，伊藤安希，竹腰進，中村直哉，新井誠，大島健一，糸川昌成，竹内晃久，上杉健太朗，鈴木芳生，水谷隆太
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場
• [Presentation] Ｘ線マイクロトモグラフィ法によるヒト大脳皮質の三次元構造解析 (2012)
  • Author(s): 水谷隆太
  • Organizer: SPring-8シンポジウム2012
  • Place of Presentation: 大阪大学コンベンションセンター
• [Presentation] フェリチンの過剰発現による生物試料の重元素標識化 (2011)
  • Author(s): 田口恵介,水谷隆太
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2011-09-23
• [Presentation] ヒトヘルペスウイルス8関連疾患におけるウイルスmicro RNAの発現 (2010)
  • Author(s): 坂本康太,福本瞳,佐藤由子,水谷隆太,佐多徹太郎,片野晴隆
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島県郷土文化会館
  • Year and Date: 2010-11-07
• [Presentation] Microtomographic Analysis of Human Brain Circuits (2010)
  • Author(s): Ryuta Mizutani, Akihisa Takeuchi, Kentaro Uesugi, Susumu Takekoshi, R. Yoshiyuki Osamura and Yoshio Suzuki
  • Organizer: 10thInternational Conference on X-Ray Microscopy
  • Place of Presentation: Chicago
  • Year and Date: 2010-08-19
• [Presentation] Microtomographic Analysis of Human Brain Circuits (2010)
  • Author(s): Ryuta Mizutani, Akihisa Takeuchi, Kentaro Uesugi, Susumu Takekoshi, R.Yoshiyuki Osamura, Yoshio Suzuki
  • Organizer: 10th International Conference on X-Ray Microscopy
  • Place of Presentation: Chicago
  • Year and Date: 2010-08-19
• [Presentation] X線マイクロCT法による脳組織の三次元構造解析 (2010)
  • Author(s): 水谷隆太,竹内晃久,上杉健太朗,竹腰進,長村義之,鈴木芳生
  • Organizer: 日本顕微鏡学会第66回学術講演会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2010-05-25
• [Presentation] X線マイクロCT法による脳組織の三次元構造解析 (2010)
  • Author(s): 水谷隆太, 竹内晃久, 上杉健太朗, 竹腰進, 長村義之, 鈴木芳生
  • Organizer: 日本顕微鏡学会第66回学術講演会
  • Place of Presentation: 名古屋国際会議場(名古屋市)
  • Year and Date: 2010-05-25
• [Presentation] 三次元テストパターンを用いたマイクロトモグラフィー装置の分解能評価 (2010)
  • Author(s): 田口恵介,水谷隆太
  • Organizer: 日本顕微鏡学会第66回学術講演会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] 放射光X線マイクロCT法による生体軟組織の三次元構造解析 (2009)
  • Author(s): 水谷隆太
  • Organizer: 第10回X線結像光学シンポジウム
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2009-11-07
• [Presentation] 放射光X線マイクロCT法による生体軟組織の三次元構造解析 (2009)
  • Author(s): 水谷隆太
  • Organizer: 第10回X線結像光学シンポジウム
  • Place of Presentation: つくば国際会議場(つくば市)
  • Year and Date: 2009-11-07
• [Presentation] KSHVのワクチン開発に関する基礎研究-KSHV粘膜ワクチン開発の可能性 (2009)
  • Author(s): 坂本康太,浅沼秀樹,中村智之,菅野隆行,水谷隆太,佐多徹太郎,片野晴隆
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 都市センターホテル
  • Year and Date: 2009-10-25
• [Book] Revealing Human Brain Circuits by Microtomography (2010)
  • Author(s): Ryuta Mizutani
  • Publisher: SPring-8 Research Frontiers 2009
• [Book] Unveiling 3D Biological Structures by X-ray Microtomography (2010)
  • Author(s): Ryuta Mizutani, Akihisa Takeuchi, Kentaro Uesugi, Susumu Takekoshi, R. Yoshiyuki Osamura and Yoshio Suzuki
  • Publisher: In Microscopy: Science, Technology, Applications and Education
• [Book] Unveiling 3D Biological Structures by X-ray Microtomography.In Microscopy : Science, Technology, Applications and Education (2010)
  • Author(s): Ryuta Mizutani, Akihisa Takeuchi, Kentaro Uesugi, Susumu Takekoshi, R.Yoshiyuki Osamura, Yoshio Suzuki
  • Publisher: Formatex Research Centre
• [Book] Three-dimensional microstructural analysis of human brain tissue by using synchrotron radiation microtomographs (2009)
  • Author(s): Ryuta Mizutani, Akihisa Takeuchi, Kentaro Uesugi, Susumu Takekoshi, R. Yoshiyuki Osamura and Yoshio Suzuki
  • Publisher: In Handbook on White Matter
• [Remarks]
  • URL: http://www.el.u-tokai.ac.jp/ryuta/
• [Remarks] Mizutani Laboratory
  • URL: http://www.el.u-tokai.ac.jp/ryuta/
• [Remarks]
  • URL: http://www.el.u-tokai.ac.jp/ryuta/
• [Remarks]
  • URL: http://www.el.u-tokai.ac.jp/ryuta/
• [Remarks]
  • URL: http://www.el.u-tokai.ac.jp/ryuta/