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Elucidation of cellobiose metabolic pathway in Trichoderma reesei

Research Project

Project/Area Number 21612002
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomass energy
Research InstitutionNagaoka University of Technology

Principal Investigator

OKADA Hirofumi  長岡技術科学大学, 工学部, 准教授 (70233343)

Co-Investigator(Renkei-kenkyūsha) OGASAWARA Wataru  長岡技術科学大学, 工学部, 准教授 (40292172)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords遺伝子 / 応用微生物 / 菌類 / 酵素 / バイオマス / β-グルコシダーゼ / セルロース / セロビオース
Research Abstract

Trichoderma reesei, which is well known to produce large amount of cellulases, expresses threeβ-glucosidases(BGLs), one of which is secreted Cel3A and the other are intracellular Cel1A and Cel3C, when grown on cellulose. To elucidate the physiological roles of these BGLs, we investigated the enzymatic properties of the recombinant enzymes produced by heterologous hosts. Furthermore, we constructed the deficient mutants of the corresponding BGL genes and characterized the amount of biomass and the cellulase and BGL activities of the mutants when grown on cellulose or cellobiose. The results demonstrated that Cel3A and Cel3C preferred pNP-Glc to cellobiose as the substrate, whereas Cel1A had higher activity for cellobiose than that for pNP-Glc. The intracellular and extracellular BGL activities of the mutants reflected the substrate specificity of the respective enzymes. We assumed that Cel1A plays the key role in cellobiose metabolism and the BGLs would not directly involve in cellulase induction. When T. reesei is grown on cellobiose, the other BGL(s) besides Cel3A seems to be expressed and secreted.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (10 results)

All 2012 2011 2010 Other

All Presentation (6 results) Remarks (4 results)

  • [Presentation] Trichoderma reesei 3種β-グルコシダーゼの生理的役割2012

    • Author(s)
      鈴木尊久
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2012-03-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] Trichoderma reesei由来3種β-グルコシダーゼの生理的役割2011

    • Author(s)
      鈴木尊久
    • Organizer
      セルラーゼ研究会
    • Place of Presentation
      花王霞ヶ浦研究所
    • Year and Date
      2011-10-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] Trichoderma reesei由来β-グルコシダーゼ(Cel3C)の酵母での発現2010

    • Author(s)
      吉川晃一
    • Organizer
      セルラーゼ研究会第24回大会
    • Place of Presentation
      花王霞ヶ浦研究所
    • Year and Date
      2010-07-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] Trichoderma reeseiにおけるセロビオース代謝に関する研究2010

    • Author(s)
      小松光子
    • Organizer
      日本農芸化学会2010年度(平成22年度)大会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] Trichoderma reesei由来3種β-グルコシダーゼの生理的役割の解明

    • Author(s)
      鈴木尊久,長戸拓也,齋藤勇司,小松光子,落合伸廣,中澤光,小笠原渉,岡田宏文
    • Organizer
      2011年セルラーゼ研究会第25回大会
    • Related Report
      2011 Final Research Report
  • [Presentation] Trichoderma reesei 3種β-グルコシダーゼの生理的役割

    • Author(s)
      鈴木尊久,長戸拓也,齋藤勇司,小松光子,落合伸廣,中澤光,小笠原渉,岡田宏文
    • Organizer
      日本農芸化学会2012年度大会
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://bio.nagaokaut.ac.jp/~okada/

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://bio.nagaokaut.ac.jp/~okada

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://bio.nagaokaut.ac.jp/~okada

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://bio.nagaokaut.ac.jp/~morikawa

    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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