Engineering of a protein that enhances cellulose saccharification
| Project/Area Number |
21612009
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomass energy
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| Research Institution | 公益財団法人岩手生物工学研究センター (2011)
Iwate Biotechnology Research Center (2009-2010) |
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Principal Investigator |
TAKEDA Takumi 公益財団法人岩手生物工学研究センター, 生物資源研究部, 主任研究員 (80423036)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | セルロース / 糖化 / エクスパンシン / タンパク質生産 / グルカナーゼ / 植物細胞壁 / 加水分解酵素 / リコンビナントタンパク質 / カタツムリ |
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| Research Abstract |
A protein(S-protein) that increased the cell wall extensibility was partially purifiedfrom Roman snail. The internal peptide sequences were determined and its cDNA wascloned. Recombinant protein produced by Aspergillus oryzae showed no hydrolyticactivity but to enhanceβ-gluca hydrolysis that was catalyzed by cell wall-degradingenzymes. The results suggest that the S-Protein, expansin-like protein, loosen theinteraction betweenβ-glucans, which was more susceptible to cell wall-degradingenzymes.
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Report
(4 results)
Research Products
(17 results)
