Single molecule analyses around the synaptic membrane formed on glass

• Project/Area Number: 21650073
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: Kyoto University
• Principal Investigator: HIRANO Tomoo 京都大学, 大学院・理学研究科, 教授 (50181178)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,470,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥700,000 (Direct Cost: ¥700,000); Fiscal Year 2009: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: シナプス / グルタミン酸受容体 / 全反射顕微鏡 / 長期増強 / 細胞培養 / 培養 / ニューレキシン / 一分子 / ダルタミン酸受容体

Research Abstract

Information transmission between neurons takes place at synapses. Here, we have succeeded to form postsynaptic membrane directly on the glass and to observe the movement of receptor molecules with high spatial and temporal resolution using total internal reflection fluorescent microscopy. We have demonstrated that different subtypes of glutamate receptor accumulate at the postsynaptic membrane through distinct routes during long-term potentiation, which is the neuronal activity dependent enhancement of synaptic transmission and has been regarded as a basis of learning and memory.

Research Products

• [Journal Article] Visualization of subunit-specific delivery of glutamate receptors to postsynaptic membrane during hippocampal long-term potentiation (2012)
  • Author(s): Tanaka H & Hirano T
  • Journal Title: Cell Reports 1 Volume: (On line掲載中) Issue: 4 Pages: 291-298
  • DOI: 10.1016/j.celrep.2012.02.004
  • NAID: 120003988278
  • Peer Reviewed
• [Journal Article] Different correlations among physiological and morphological properties at single glutamatergic synapses in the rat hippocampus and the cerebellum. (2011)
  • Author(s): Miyawaki, H., Hirano, T.
  • Journal Title: Synapse Volume: 65 Pages: 412-423
  • NAID: 120005593571
  • Peer Reviewed
• [Journal Article] Different correlations among physiological and morphological properties at single glutamatergic synapses in the rat hippocampus and the cerebellum (2010)
  • Author(s): Miyawaki H & Hirano T
  • Journal Title: Synapse 65 Volume: 65 Issue: 5 Pages: 412-423
  • DOI: 10.1002/syn.20860
  • NAID: 120005593571
  • Year and Date: 2010-10-20
  • Peer Reviewed
• [Journal Article] Ontogeny-recapitulating generation and tissue integration of ES cell-derived Purkinje cells (2010)
  • Author(s): Muguruma K, Nishiyama A, Ono, Y, Miyawaki H, Mizuhara E, Hori S, Kakizuka A, Obata K, Yanagawa Y, Hirano T & Sasai, Y
  • Journal Title: Nature Neurosci Volume: 13 Issue: 10 Pages: 1171-1180
  • DOI: 10.1038/nn.2638
  • Year and Date: 2010-09-12
  • Peer Reviewed
• [Journal Article] Flap loop of GluD2 binds to Cbln1 and induces presynaptic differentiation (2010)
  • Author(s): Kuroyanagi T & Hirano T
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 398 Issue: 3 Pages: 537-541
  • DOI: 10.1016/j.bbrc.2010.06.114
  • NAID: 120002511328
  • Peer Reviewed
• [Journal Article] Pre-and postsynaptic neuronal activity supports the axon development of callosal projection neurons during different postnatal periods in the mouse cerebral cortex (2010)
  • Author(s): Mizuno, H., Hirano, T. and Tagawa, Y.
  • Journal Title: Eur. J. Neurosci Volume: 31 Issue: 3 Pages: 410-424
  • DOI: 10.1111/j.1460-9568.2009.07070.x
  • Peer Reviewed
• [Journal Article] Flap loop of GluD2 binds to Cbln1 and induces presynaptic differentiation. (2010)
  • Author(s): Kuroyanagi, T., Hirano, T.
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 398 Pages: 537-541
  • NAID: 120002511328
  • Peer Reviewed
• [Journal Article] Ontogeny-recapitulating generation and tissue integration of ES cell-derived Purkinje cells. (2010)
  • Author(s): Muguruma, K., Nishiyama, A., Ono, Y., Miyawaki, H., Mizuhara, E., Hori, S., Kakizuka, A., Obata, K., Yanagawa, Y., Hirano, T., Sasai, Y
  • Journal Title: Nature Neurosci. Volume: 13 Pages: 1171-1180
  • Peer Reviewed
• [Journal Article] Pre- and postsynaptic neuronal activity supports the axon development of callosal projection neurons during different postnatal periods in the mouse cerebral cortex (2010)
  • Author(s): Mizuno, H., Hirano, T., Tagawa, Y.
  • Journal Title: Eur.J.Neurosci. 31 Pages: 410-424
  • Peer Reviewed
• [Journal Article] Control of cortical axon elongation by a GABA-driven Ca2+ calmodulin-dependent protein kinase cascade (2009)
  • Author(s): Ageta-Ishihara N, Takemoto-Kimura S, Nonaka M, Adachi-Morishima A, Suzuki K, Kamijo S, Fujii H, Mano T, Blaeser F, Chatila T, Mizuno H, Hirano T, Tagawa Y, Okuno H & Bito H
  • Journal Title: J. Neurosci Volume: 29 Pages: 13720-13729
  • Peer Reviewed
• [Journal Article] Prediction and validation of a mechanism to control the threshold for inhibitory synaptic plasticity (2009)
  • Author(s): Kitagawa Y, Hirano T & Kawaguchi S
  • Journal Title: Mol. Systems. Biol Volume: 5 Issue: 1 Pages: 1-16
  • DOI: 10.1038/msb.2009.39
  • Peer Reviewed
• [Journal Article] Prediction and validation of a mechanism to control the threshold for inhibitory synaptic plasticity (2009)
  • Author(s): Kitagawa, Y., Hirano, T., Kawaguchi, S.
  • Journal Title: Mol.Syst.Biol. 5 Pages: 1-16
  • Peer Reviewed
• [Journal Article] Control of cortical axon elongation by a GABA-driven Ca^<2+> calmodulin-dependent protein kinase cascade (2009)
  • Author(s): Ageta-Ishihara, N., et al.
  • Journal Title: J.Neurosci. 29 Pages: 13720-13729
  • Peer Reviewed
• [Presentation] Subunit-specific translocation of AMPA receptors to post-synaptic membrane during hippocampal LTP. Neuroscience 201 (2011)
  • Author(s): Tanaka H & Hirano T
  • Organizer: Washington Convention Center
  • Place of Presentation: 米国
  • Year and Date: 2011-11-16
• [Presentation] Subunit-specific translocation of AMPA receptors to post-synaptic membrane during hippocampal LTP (2011)
  • Author(s): Tanaka H, Hirano T
  • Organizer: Neuroscience 2011
  • Place of Presentation: Washington Convention Center(米国)
  • Year and Date: 2011-11-16
• [Presentation] Subunit-specific translocation of AMPA receptors during hippocampal LTP (2011)
  • Author(s): Tanaka H & Hirano T
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-09-17
• [Presentation] Subunit-specific translocation of AMPA receptors during hippocampal LTP (2011)
  • Author(s): Tanaka H, Hirano T
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-09-17
• [Presentation] Real-time imaging of glutamate receptor movement around postsynaptic membrane (2010)
  • Author(s): Tanaka H & Hirano T
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセン
  • Year and Date: 2010-09-03
• [Presentation] Real-time imaging of glutamate receptor movement around postsynaptic membrane. (2010)
  • Author(s): Tanaka H., Hirano, T.
  • Organizer: Neuro 2010
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2010-09-03
• [Presentation] Roles of glutamate receptor delta family in synapse formation. Symposium"Novel aspects of the function of ionotropic & metabotropic glutamate receptors (2009)
  • Author(s): Hirano T
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-09-16
• [Presentation] Roles of glutamate receptor δ family in synapse formation (2009)
  • Author(s): Hirano, T.
  • Organizer: Symposium "Novel aspects of the function of ionotropic & metabotropic glutamate receptors" in the 32nd annual meeting of the Japan Neuroscience Society
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-09-16
• [Remarks] 研究室のホームページ
  • URL: http://www.neurosci.biophys.kyoto-u.ac.jp/main.html
• [Remarks] 本研究成果については、京都大学でプレスリリースを行った。プレスリリースに関しての情報は下記ホームページに掲載された。
  • URL: http://www.kyoto-u.ac.jp/ja/news_data/h/h1/news6/2011/120323_1.htm
• [Remarks]
  • URL: http://www.kyoto-u.ac.jp/ja/news_data/h/h1/news6/2011/120323_1.htm
• [Remarks]
  • URL: http://www.neurosci.biophys.kyoto-u.ac.jp/main.html
• [Remarks]
  • URL: http://www.neurosci.biophys.kyoto-u.ac.jp/main.html