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Study on development of in situ tip-enhanced Raman spectroscopy for characterizing the surface and inside of a living stem cells.

Research Project

Project/Area Number 21650104
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Biomedical engineering/Biological material science
Research InstitutionThe University of Electro-Communications (2010-2011)
Hokkaido University (2009)

Principal Investigator

YAMAMOTO Sadaaki  電気通信大学, 燃料電池イノベーション研究センター, 特任教授 (20374720)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,520,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥120,000)
Fiscal Year 2011: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2010: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2009: ¥2,500,000 (Direct Cost: ¥2,500,000)
Keywords細胞培養基材 / 細胞・組織 / 走査プローブ顕微鏡 / 移植・再生医療 / 表面・界面物性 / 計測
Research Abstract

Mesenchymal stem cells from rat adult bone marrow(r-MSCs) were cultured on Honeycomb films and FI-PEARL films without cytkines for cell growth. The anomalous cellular adhesion and division were found in such a way that r-MSCs did not spread at all keeping semispherical original shape along culture and divided directly in the semispherical cell bodies. The proliferative ability on PE-PEARL films was higher than that on a commercially available polystyrene culture plate. These films enabled easy recovery of MSCs by rinsing with water without the conventional trypsin treatment. The present study suggested that both films are promising scaffolds for mesenchymal stem cells which make the bio risk free subculture of MSCs possible. We obtained technical data useful for constructing a sophisticated technique based on tip-enhanced Rama scattering for characterizing the surface and inside of a living mesenchymal stem cell.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (6 results)

All 2010 2009

All Journal Article (1 results) Presentation (4 results) Book (1 results)

  • [Journal Article] 自己組織化ハニカムフィルムに対するラット骨髄間葉系幹細胞の応答2010

    • Author(s)
      森田有香, 山本貞明, 藪浩, 伊藤絵美子, 本望修, 居城邦治, 下村政嗣
    • Journal Title

      表面科学

      Volume: 31 Pages: 392-399

    • NAID

      10026546017

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 自己組織化ハニカムパターン多孔性高分子薄膜による細胞制御”2010

    • Author(s)
      山本貞明
    • Organizer
      09-5 ポリマーフロンティア21
    • Place of Presentation
      東工大百年記念館フェライト会議室
    • Year and Date
      2010-01-22
    • Related Report
      2011 Final Research Report
  • [Presentation] 自己組織化ハニカムパターン多孔性高分子薄膜による細胞制御2010

    • Author(s)
      山本貞明
    • Organizer
      09-5ポリマーフロンティア21
    • Place of Presentation
      東工大百年記念館 フェライト会議室
    • Year and Date
      2010-01-22
    • Related Report
      2009 Annual Research Report
  • [Presentation] Honeycomb-Patterned Polymer Films Enhance the Proliferation of Mesenchymal Stem Cell from Rat Adult Bone Marrow2009

    • Author(s)
      Y. Morita, S. Yamamoto, H. Yabu, E. Ito, K. Ijiro, O. Honmou and M. Shimomrua
    • Organizer
      The 1st FAPS Polymer Congress
    • Place of Presentation
      Nagoya
    • Year and Date
      2009-10-20
    • Related Report
      2011 Final Research Report
  • [Presentation] Honeycomb-patterned topography regulates cell shape and improves proliferative ability of multipotentadult stem cells2009

    • Author(s)
      Y. Morita, S. Yamamoto, H. Yabub, E. Ito, M, Tanaka, K. Ijiro, O. Honmoue, M. Shimomura
    • Organizer
      Praque Czech Republic
    • Place of Presentation
      Praque Czech Republic
    • Year and Date
      2009-07-05
    • Related Report
      2011 Final Research Report
  • [Book] 日本化学会偏現代界面コロイド科学の辞典2010

    • Author(s)
      山本貞明
    • Publisher
      再生医療の細胞培養基板-ハニカムフィルム
    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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